ID   BCSE_KLEP7              Reviewed;         517 AA.
AC   A6TFD9;
DT   25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2017, sequence version 2.
DT   25-MAY-2022, entry version 55.
DE   RecName: Full=Cyclic di-GMP binding protein BcsE {ECO:0000303|PubMed:24942809};
DE   AltName: Full=Cellulose biosynthesis protein BcsE;
GN   Name=bcsE; ORFNames=KPN_03886;
OS   Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=272620;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700721 / MGH 78578;
RG   The Klebsiella pneumonia Genome Sequencing Project;
RA   McClelland M., Sanderson E.K., Spieth J., Clifton W.S., Latreille P.,
RA   Sabo A., Pepin K., Bhonagiri V., Porwollik S., Ali J., Wilson R.K.;
RL   Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, C-DI-GMP-BINDING, AND MUTAGENESIS OF ARG-411 AND ASP-414.
RC   STRAIN=ATCC 700721;
RX   PubMed=24942809; DOI=10.1111/mmi.12672;
RA   Fang X., Ahmad I., Blanka A., Schottkowski M., Cimdins A., Galperin M.Y.,
RA   Roemling U., Gomelsky M.;
RT   "GIL, a new c-di-GMP-binding protein domain involved in regulation of
RT   cellulose synthesis in enterobacteria.";
RL   Mol. Microbiol. 93:439-452(2014).
CC   -!- FUNCTION: Required for cellulose biosynthesis (By similarity). Binds
CC       bis-(3'-5') cyclic diguanylic acid (c-di-GMP) (PubMed:24942809).
CC       {ECO:0000250|UniProtKB:Q8ZLB5, ECO:0000269|PubMed:24942809}.
CC   -!- SIMILARITY: Belongs to the BcsE family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABR79273.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; CP000647; ABR79273.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_002921516.1; NC_009648.1.
DR   AlphaFoldDB; A6TFD9; -.
DR   SMR; A6TFD9; -.
DR   STRING; 272620.KPN_03886; -.
DR   EnsemblBacteria; ABR79273; ABR79273; KPN_03886.
DR   KEGG; kpn:KPN_03886; -.
DR   HOGENOM; CLU_039389_2_0_6; -.
DR   Proteomes; UP000000265; Chromosome.
DR   GO; GO:0035438; F:cyclic-di-GMP binding; IEA:InterPro.
DR   GO; GO:0030244; P:cellulose biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR017745; BcsE.
DR   Pfam; PF10995; CBP_GIL; 1.
DR   TIGRFAMs; TIGR03369; cellulose_bcsE; 1.
PE   1: Evidence at protein level;
KW   Cellulose biosynthesis; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..517
FT                   /note="Cyclic di-GMP binding protein BcsE"
FT                   /id="PRO_0000441758"
FT   MUTAGEN         411
FT                   /note="R->D: No longer binds c-di-GMP."
FT                   /evidence="ECO:0000269|PubMed:24942809"
FT   MUTAGEN         414
FT                   /note="D->R: No longer binds c-di-GMP."
FT                   /evidence="ECO:0000269|PubMed:24942809"
SQ   SEQUENCE   517 AA;  58614 MW;  1D5ECC45689F1C2B CRC64;
     MDNVFTLGIS SLWDEVCHMP VGGVWWLNVD RYADAVSLFN QTLAAQAKNS HVAALVMGNK
     PKDIISLDHT HGPDNIALFT LPNRPQALEE IHRDLVCSLE PGNYLFILLC AENAWQNINN
     EKLCAWVEKT SRWAQYHRCA FLAINSAQDI DRQLTPLLRE YRSLSGLASI RYQGDRHIFD
     IAWWGSDKGI SAQQQLMVQH DDAGWRLAQD AETSVQPRSD EKAILSHVRV LEGAPPLSEY
     WTLFDTNDEV FNAGRTAQAA TILFSITQNT QIEQLGRYIH TLRRQRGTAL KIIVREQTPS
     LRATDERLLL SSGASLVIPS SASLSRCLTL IESVQNQKFS RHIPEDFATL LTWSQPLKLR
     GYQKWDAFCE AVHNVMTNTL LPPDSKGVMV ALRPAPGLRV EQALTLCKPN RMGDIMTIGN
     NRLVLFLSFC RINDLDTALN HIFPLPTGDI FSNRMVWFED KQILSEIVIM RGVEPARWNT
     PLPLSVGKNE TINATHDGRH WRRYPEPHRL TTREEQA