ID   RUTE_ECO5E              Reviewed;         196 AA.
AC   B5YU50;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   25-NOV-2008, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Probable malonic semialdehyde reductase RutE {ECO:0000255|HAMAP-Rule:MF_01204};
DE            EC=1.1.1.298 {ECO:0000255|HAMAP-Rule:MF_01204};
GN   Name=rutE {ECO:0000255|HAMAP-Rule:MF_01204};
GN   OrderedLocusNames=ECH74115_1245;
OS   Escherichia coli O157:H7 (strain EC4115 / EHEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=444450;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=EC4115 / EHEC;
RX   PubMed=22135463; DOI=10.1073/pnas.1107176108;
RA   Eppinger M., Mammel M.K., Leclerc J.E., Ravel J., Cebula T.A.;
RT   "Genomic anatomy of Escherichia coli O157:H7 outbreaks.";
RL   Proc. Natl. Acad. Sci. U.S.A. 108:20142-20147(2011).
CC   -!- FUNCTION: May reduce toxic product malonic semialdehyde to 3-
CC       hydroxypropionic acid, which is excreted. {ECO:0000255|HAMAP-
CC       Rule:MF_01204}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-hydroxypropanoate + NADP(+) = 3-oxopropanoate + H(+) +
CC         NADPH; Xref=Rhea:RHEA:26438, ChEBI:CHEBI:15378, ChEBI:CHEBI:16510,
CC         ChEBI:CHEBI:33190, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC         EC=1.1.1.298; Evidence={ECO:0000255|HAMAP-Rule:MF_01204};
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01204};
CC   -!- INDUCTION: Up-regulated by the nitrogen regulatory protein C (NtrC also
CC       called GlnG) and repressed by RutR. {ECO:0000255|HAMAP-Rule:MF_01204}.
CC   -!- SIMILARITY: Belongs to the nitroreductase family. HadB/RutE subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01204}.
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DR   EMBL; CP001164; ACI36934.1; -; Genomic_DNA.
DR   RefSeq; WP_001001189.1; NC_011353.1.
DR   AlphaFoldDB; B5YU50; -.
DR   SMR; B5YU50; -.
DR   KEGG; ecf:ECH74115_1245; -.
DR   HOGENOM; CLU_084441_0_0_6; -.
DR   OMA; LMVVNIG; -.
DR   GO; GO:0035527; F:3-hydroxypropionate dehydrogenase (NADP+) activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019740; P:nitrogen utilization; IEA:UniProtKB-UniRule.
DR   GO; GO:0006212; P:uracil catabolic process; IEA:UniProtKB-UniRule.
DR   CDD; cd02148; RutE-like; 1.
DR   Gene3D; 3.40.109.10; -; 1.
DR   HAMAP; MF_01204; Oxidoreductase_RutE_HadB; 1.
DR   InterPro; IPR029479; Nitroreductase.
DR   InterPro; IPR000415; Nitroreductase-like.
DR   InterPro; IPR023936; RutE-like.
DR   Pfam; PF00881; Nitroreductase; 1.
DR   SUPFAM; SSF55469; SSF55469; 1.
PE   3: Inferred from homology;
KW   Flavoprotein; FMN; NAD; NADP; Oxidoreductase.
FT   CHAIN           1..196
FT                   /note="Probable malonic semialdehyde reductase RutE"
FT                   /id="PRO_1000138690"
SQ   SEQUENCE   196 AA;  21565 MW;  951FAB69BE527571 CRC64;
     MNEAVSPGAL STLFTDARTH NGWRETPVSD ETLRELYALM KWGPTSANCS PARIVFIRTA
     EGKERLRPAL SSGNLQKTLT APVTAIVAWD SEFYERLPQL FPHGDARSWF TSSPQLAEET
     AFRNSSMQAA YLIIACRALG LDTGPMSGFD RQHVDDAFFA GSTLKSNLLI NIGYGDSSKL
     FARLPRLSFE EACGLL