ID   RUTE_ECOK1              Reviewed;         196 AA.
AC   A1A9R3;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Probable malonic semialdehyde reductase RutE {ECO:0000255|HAMAP-Rule:MF_01204};
DE            EC=1.1.1.298 {ECO:0000255|HAMAP-Rule:MF_01204};
GN   Name=rutE {ECO:0000255|HAMAP-Rule:MF_01204}; OrderedLocusNames=Ecok1_09090;
GN   ORFNames=APECO1_99;
OS   Escherichia coli O1:K1 / APEC.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=405955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=17293413; DOI=10.1128/jb.01726-06;
RA   Johnson T.J., Kariyawasam S., Wannemuehler Y., Mangiamele P., Johnson S.J.,
RA   Doetkott C., Skyberg J.A., Lynne A.M., Johnson J.R., Nolan L.K.;
RT   "The genome sequence of avian pathogenic Escherichia coli strain O1:K1:H7
RT   shares strong similarities with human extraintestinal pathogenic E. coli
RT   genomes.";
RL   J. Bacteriol. 189:3228-3236(2007).
CC   -!- FUNCTION: May reduce toxic product malonic semialdehyde to 3-
CC       hydroxypropionic acid, which is excreted. {ECO:0000255|HAMAP-
CC       Rule:MF_01204}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-hydroxypropanoate + NADP(+) = 3-oxopropanoate + H(+) +
CC         NADPH; Xref=Rhea:RHEA:26438, ChEBI:CHEBI:15378, ChEBI:CHEBI:16510,
CC         ChEBI:CHEBI:33190, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC         EC=1.1.1.298; Evidence={ECO:0000255|HAMAP-Rule:MF_01204};
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01204};
CC   -!- INDUCTION: Up-regulated by the nitrogen regulatory protein C (NtrC also
CC       called GlnG) and repressed by RutR. {ECO:0000255|HAMAP-Rule:MF_01204}.
CC   -!- SIMILARITY: Belongs to the nitroreductase family. HadB/RutE subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01204}.
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DR   EMBL; CP000468; ABJ00403.1; -; Genomic_DNA.
DR   RefSeq; WP_001001168.1; NC_008563.1.
DR   AlphaFoldDB; A1A9R3; -.
DR   SMR; A1A9R3; -.
DR   EnsemblBacteria; ABJ00403; ABJ00403; APECO1_99.
DR   KEGG; ecv:APECO1_99; -.
DR   HOGENOM; CLU_084441_0_0_6; -.
DR   OMA; THNGWLD; -.
DR   Proteomes; UP000008216; Chromosome.
DR   GO; GO:0035527; F:3-hydroxypropionate dehydrogenase (NADP+) activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019740; P:nitrogen utilization; IEA:UniProtKB-UniRule.
DR   GO; GO:0006212; P:uracil catabolic process; IEA:UniProtKB-UniRule.
DR   CDD; cd02148; RutE-like; 1.
DR   Gene3D; 3.40.109.10; -; 1.
DR   HAMAP; MF_01204; Oxidoreductase_RutE_HadB; 1.
DR   InterPro; IPR029479; Nitroreductase.
DR   InterPro; IPR000415; Nitroreductase-like.
DR   InterPro; IPR023936; RutE-like.
DR   Pfam; PF00881; Nitroreductase; 1.
DR   SUPFAM; SSF55469; SSF55469; 1.
PE   3: Inferred from homology;
KW   Flavoprotein; FMN; NAD; NADP; Oxidoreductase.
FT   CHAIN           1..196
FT                   /note="Probable malonic semialdehyde reductase RutE"
FT                   /id="PRO_1000066136"
SQ   SEQUENCE   196 AA;  21625 MW;  D74414DB8B363879 CRC64;
     MNEAVSPGAL STLFTDARTH NGWRETPVSD ETLREIYALM KWGPTSANCS PARIVFIRTA
     EGKERLRPAL SSGNLQKTLT APVTAIVAWD SEFYERLPQL FPHGDARSWF TSSPQLAEET
     AFRNSSMQAA YLIFACRALG LDTGPMSGFD RQYVDDAFFA GSTLKSNLLI NIGYGDSSKL
     FARLPRLSFE EACGLL