ID   RUTE_SHIF8              Reviewed;         196 AA.
AC   Q0T626;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Probable malonic semialdehyde reductase RutE {ECO:0000255|HAMAP-Rule:MF_01204};
DE            EC=1.1.1.298 {ECO:0000255|HAMAP-Rule:MF_01204};
GN   Name=rutE {ECO:0000255|HAMAP-Rule:MF_01204}; OrderedLocusNames=SFV_1020;
OS   Shigella flexneri serotype 5b (strain 8401).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=373384;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=8401;
RX   PubMed=16822325; DOI=10.1186/1471-2164-7-173;
RA   Nie H., Yang F., Zhang X., Yang J., Chen L., Wang J., Xiong Z., Peng J.,
RA   Sun L., Dong J., Xue Y., Xu X., Chen S., Yao Z., Shen Y., Jin Q.;
RT   "Complete genome sequence of Shigella flexneri 5b and comparison with
RT   Shigella flexneri 2a.";
RL   BMC Genomics 7:173-173(2006).
CC   -!- FUNCTION: May reduce toxic product malonic semialdehyde to 3-
CC       hydroxypropionic acid, which is excreted. {ECO:0000255|HAMAP-
CC       Rule:MF_01204}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-hydroxypropanoate + NADP(+) = 3-oxopropanoate + H(+) +
CC         NADPH; Xref=Rhea:RHEA:26438, ChEBI:CHEBI:15378, ChEBI:CHEBI:16510,
CC         ChEBI:CHEBI:33190, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC         EC=1.1.1.298; Evidence={ECO:0000255|HAMAP-Rule:MF_01204};
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01204};
CC   -!- INDUCTION: Up-regulated by the nitrogen regulatory protein C (NtrC also
CC       called GlnG) and repressed by RutR. {ECO:0000255|HAMAP-Rule:MF_01204}.
CC   -!- SIMILARITY: Belongs to the nitroreductase family. HadB/RutE subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01204}.
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DR   EMBL; CP000266; ABF03239.1; -; Genomic_DNA.
DR   RefSeq; WP_001001165.1; NC_008258.1.
DR   AlphaFoldDB; Q0T626; -.
DR   SMR; Q0T626; -.
DR   EnsemblBacteria; ABF03239; ABF03239; SFV_1020.
DR   GeneID; 58391248; -.
DR   KEGG; sfv:SFV_1020; -.
DR   HOGENOM; CLU_084441_0_0_6; -.
DR   OMA; LMVVNIG; -.
DR   BioCyc; SFLE373384:SFV_RS05640-MON; -.
DR   Proteomes; UP000000659; Chromosome.
DR   GO; GO:0035527; F:3-hydroxypropionate dehydrogenase (NADP+) activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019740; P:nitrogen utilization; IEA:UniProtKB-UniRule.
DR   GO; GO:0006212; P:uracil catabolic process; IEA:UniProtKB-UniRule.
DR   CDD; cd02148; RutE-like; 1.
DR   Gene3D; 3.40.109.10; -; 1.
DR   HAMAP; MF_01204; Oxidoreductase_RutE_HadB; 1.
DR   InterPro; IPR029479; Nitroreductase.
DR   InterPro; IPR000415; Nitroreductase-like.
DR   InterPro; IPR023936; RutE-like.
DR   Pfam; PF00881; Nitroreductase; 1.
DR   SUPFAM; SSF55469; SSF55469; 1.
PE   3: Inferred from homology;
KW   Flavoprotein; FMN; NAD; NADP; Oxidoreductase.
FT   CHAIN           1..196
FT                   /note="Probable malonic semialdehyde reductase RutE"
FT                   /id="PRO_1000066146"
SQ   SEQUENCE   196 AA;  21508 MW;  0FEE1928B24EA540 CRC64;
     MNEAVSPGAL STLFTDARTH NGWRETPVSD ETLQELYALM KWGPTSANCS PARIVFIRTA
     EGKERLRPAL SSGNLQKTLT APVTAIVAWD SEFYERLPLL FPHGDARSWF TSSPQLAEET
     AFRNSSMQAA YLIVACRALG LDTGPMSGFD RQHVDDAFFA GSTLKSNLLI NIGYGDSSKL
     FARLPRLSFE EACGLL