BCSF_ECOLI
ID BCSF_ECOLI Reviewed; 63 AA.
AC P0ADJ5; P37658; Q2M7J8;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 27-JUN-2006, sequence version 2.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Cellulose biosynthesis protein BcsF;
GN Name=bcsF; Synonyms=yhjT; OrderedLocusNames=b3537, JW5663;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8041620; DOI=10.1093/nar/22.13.2576;
RA Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT "Analysis of the Escherichia coli genome. V. DNA sequence of the region
RT from 76.0 to 81.5 minutes.";
RL Nucleic Acids Res. 22:2576-2586(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP DISRUPTION PHENOTYPE.
RC STRAIN=K12 / W3110 / AR3110;
RX PubMed=24097954; DOI=10.1128/jb.00946-13;
RA Serra D.O., Richter A.M., Hengge R.;
RT "Cellulose as an architectural element in spatially structured Escherichia
RT coli biofilms.";
RL J. Bacteriol. 195:5540-5554(2013).
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass
CC membrane protein {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: When the bcsEFG operon is disrupted in a
CC cellulose-synthesizing strain (a strain K12 / W3110 derivative called
CC AR3110 with a restored, functional bcsQ gene), cellulose is no longer
CC made. {ECO:0000269|PubMed:24097954}.
CC -!- MISCELLANEOUS: Cellulose production is abolished in E.coli K12 / MG1655
CC and W3110 due to a premature stop codon in bcsQ (PubMed:24097954).
CC {ECO:0000305|PubMed:24097954}.
CC -!- SIMILARITY: Belongs to the BcsF family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB18515.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; U00039; AAB18515.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC76562.2; -; Genomic_DNA.
DR EMBL; AP009048; BAE77758.1; -; Genomic_DNA.
DR PIR; S47759; S47759.
DR RefSeq; NP_417994.2; NC_000913.3.
DR RefSeq; WP_000988308.1; NZ_STEB01000018.1.
DR AlphaFoldDB; P0ADJ5; -.
DR BioGRID; 4260851; 219.
DR IntAct; P0ADJ5; 2.
DR STRING; 511145.b3537; -.
DR PaxDb; P0ADJ5; -.
DR PRIDE; P0ADJ5; -.
DR EnsemblBacteria; AAC76562; AAC76562; b3537.
DR EnsemblBacteria; BAE77758; BAE77758; BAE77758.
DR GeneID; 66672578; -.
DR GeneID; 948059; -.
DR KEGG; ecj:JW5663; -.
DR KEGG; eco:b3537; -.
DR PATRIC; fig|511145.12.peg.3648; -.
DR EchoBASE; EB2173; -.
DR eggNOG; ENOG5032ZX1; Bacteria.
DR HOGENOM; CLU_198346_0_0_6; -.
DR OMA; KANRKHD; -.
DR BioCyc; EcoCyc:EG12264-MON; -.
DR PRO; PR:P0ADJ5; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030244; P:cellulose biosynthetic process; IEA:UniProtKB-KW.
DR InterPro; IPR019995; Cellulose_BcsF/YhjT.
DR Pfam; PF11120; CBP_BcsF; 1.
DR TIGRFAMs; TIGR03493; cellullose_BcsF; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Cellulose biosynthesis; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..63
FT /note="Cellulose biosynthesis protein BcsF"
FT /id="PRO_0000169581"
FT TRANSMEM 4..24
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 63 AA; 7364 MW; 7CA2611DCE4CA155 CRC64;
MMTISDIIEI IVVCALIFFP LGYLARHSLR RIRDTLRLFF AKPRYVKPAG TLRRTEKARA
TKK
