ID   RUTF_ECO57              Reviewed;         164 AA.
AC   Q8XAV0;
DT   02-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT   02-MAY-2002, sequence version 2.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=FMN reductase (NADH) RutF;
DE            EC=1.5.1.42;
DE   AltName: Full=FMN reductase;
DE   AltName: Full=NADH-flavin reductase RutF;
DE   AltName: Full=NADH:flavin oxidoreductase;
GN   Name=rutF; OrderedLocusNames=Z1506, ECs1253;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Catalyzes the reduction of FMN to FMNH2 which is used to
CC       reduce pyrimidine by RutA via the Rut pathway. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=FMNH2 + NAD(+) = FMN + 2 H(+) + NADH; Xref=Rhea:RHEA:21620,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57618,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:58210; EC=1.5.1.42;
CC   -!- INDUCTION: Up-regulated by the nitrogen regulatory protein C (NtrC also
CC       called GlnG) and repressed by RutR. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the non-flavoprotein flavin reductase family.
CC       RutF subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAG55623.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC       Sequence=BAB34676.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE005174; AAG55623.1; ALT_INIT; Genomic_DNA.
DR   EMBL; BA000007; BAB34676.1; ALT_INIT; Genomic_DNA.
DR   PIR; C85645; C85645.
DR   PIR; E90785; E90785.
DR   RefSeq; NP_309280.2; NC_002695.1.
DR   RefSeq; WP_001028088.1; NZ_SEKU01000016.1.
DR   AlphaFoldDB; Q8XAV0; -.
DR   SMR; Q8XAV0; -.
DR   STRING; 155864.EDL933_1428; -.
DR   EnsemblBacteria; AAG55623; AAG55623; Z1506.
DR   EnsemblBacteria; BAB34676; BAB34676; ECs_1253.
DR   GeneID; 912887; -.
DR   KEGG; ece:Z1506; -.
DR   KEGG; ecs:ECs_1253; -.
DR   PATRIC; fig|386585.9.peg.1356; -.
DR   eggNOG; COG1853; Bacteria.
DR   HOGENOM; CLU_059021_2_2_6; -.
DR   OMA; WFDRGYH; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR   GO; GO:0052874; F:FMN reductase (NADH) activity; IEA:RHEA.
DR   GO; GO:0008752; F:FMN reductase activity; IEA:InterPro.
DR   GO; GO:0042602; F:riboflavin reductase (NADPH) activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019740; P:nitrogen utilization; IEA:UniProtKB-UniRule.
DR   GO; GO:0006212; P:uracil catabolic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.30.110.10; -; 1.
DR   HAMAP; MF_00833; RutF; 1.
DR   InterPro; IPR002563; Flavin_Rdtase-like_dom.
DR   InterPro; IPR019917; RutF.
DR   InterPro; IPR012349; Split_barrel_FMN-bd.
DR   Pfam; PF01613; Flavin_Reduct; 1.
DR   SMART; SM00903; Flavin_Reduct; 1.
DR   TIGRFAMs; TIGR03615; RutF; 1.
PE   3: Inferred from homology;
KW   Flavoprotein; FMN; NAD; Oxidoreductase; Reference proteome.
FT   CHAIN           1..164
FT                   /note="FMN reductase (NADH) RutF"
FT                   /id="PRO_0000085538"
SQ   SEQUENCE   164 AA;  17760 MW;  78778237EF228380 CRC64;
     MNIVDQQTFR DAMSCMGAAV NIITTDGPAG RAGFTASAVC SVTDTPPTLL VCLNRGASVW
     PVFNENRTLC VNTLSAGQEP LSNLFGGKTP MEHRFAAARW QNGVTGCPQL EEALVSFDCR
     ISQVVSVGTH DILFCAIEAI HRHATPYGLV WFDRSYHALM RPAC