BCSG_ECOLI
ID BCSG_ECOLI Reviewed; 559 AA.
AC P37659; Q2M7J9;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Cellulose biosynthesis protein BcsG;
GN Name=bcsG; Synonyms=yhjU; OrderedLocusNames=b3538, JW3506;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8041620; DOI=10.1093/nar/22.13.2576;
RA Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT "Analysis of the Escherichia coli genome. V. DNA sequence of the region
RT from 76.0 to 81.5 minutes.";
RL Nucleic Acids Res. 22:2576-2586(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP DISRUPTION PHENOTYPE.
RC STRAIN=K12 / W3110 / AR3110;
RX PubMed=24097954; DOI=10.1128/jb.00946-13;
RA Serra D.O., Richter A.M., Hengge R.;
RT "Cellulose as an architectural element in spatially structured Escherichia
RT coli biofilms.";
RL J. Bacteriol. 195:5540-5554(2013).
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: When the bcsEFG operon is disrupted in a
CC cellulose-synthesizing strain (a strain K12 / W3110 derivative called
CC AR3110 with a restored, functional bcsQ gene), cellulose is no longer
CC made. {ECO:0000269|PubMed:24097954}.
CC -!- MISCELLANEOUS: Cellulose production is abolished in E.coli K12 / MG1655
CC and W3110 due to a premature stop codon in bcsQ (PubMed:24097954).
CC {ECO:0000305|PubMed:24097954}.
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DR EMBL; U00039; AAB18516.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76563.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77757.1; -; Genomic_DNA.
DR PIR; S47760; S47760.
DR RefSeq; NP_417995.1; NC_000913.3.
DR RefSeq; WP_000191622.1; NZ_LN832404.1.
DR PDB; 6PCZ; X-ray; 1.44 A; A/B=164-559.
DR PDB; 6PD0; X-ray; 1.75 A; A/B=164-559.
DR PDBsum; 6PCZ; -.
DR PDBsum; 6PD0; -.
DR AlphaFoldDB; P37659; -.
DR SMR; P37659; -.
DR BioGRID; 4263173; 2.
DR DIP; DIP-12392N; -.
DR IntAct; P37659; 3.
DR STRING; 511145.b3538; -.
DR TCDB; 9.B.275.1.1; the phosphoethanolamine transferase (peat) family.
DR jPOST; P37659; -.
DR PaxDb; P37659; -.
DR PRIDE; P37659; -.
DR EnsemblBacteria; AAC76563; AAC76563; b3538.
DR EnsemblBacteria; BAE77757; BAE77757; BAE77757.
DR GeneID; 948058; -.
DR KEGG; ecj:JW3506; -.
DR KEGG; eco:b3538; -.
DR PATRIC; fig|1411691.4.peg.3178; -.
DR EchoBASE; EB2174; -.
DR eggNOG; COG2194; Bacteria.
DR HOGENOM; CLU_024049_1_0_6; -.
DR OMA; GDKMQMS; -.
DR PhylomeDB; P37659; -.
DR BioCyc; EcoCyc:EG12265-MON; -.
DR BioCyc; MetaCyc:EG12265-MON; -.
DR PRO; PR:P37659; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0030244; P:cellulose biosynthetic process; IEA:UniProtKB-KW.
DR InterPro; IPR017744; BcsG.
DR Pfam; PF11658; CBP_BcsG; 1.
DR TIGRFAMs; TIGR03368; cellulose_yhjU; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell inner membrane; Cell membrane; Cellulose biosynthesis;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..559
FT /note="Cellulose biosynthesis protein BcsG"
FT /id="PRO_0000169584"
FT TRANSMEM 34..54
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 68..88
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 138..158
FT /note="Helical"
FT /evidence="ECO:0000255"
FT HELIX 203..218
FT /evidence="ECO:0007829|PDB:6PCZ"
FT STRAND 236..244
FT /evidence="ECO:0007829|PDB:6PCZ"
FT HELIX 247..252
FT /evidence="ECO:0007829|PDB:6PCZ"
FT HELIX 259..262
FT /evidence="ECO:0007829|PDB:6PCZ"
FT STRAND 265..272
FT /evidence="ECO:0007829|PDB:6PCZ"
FT HELIX 278..286
FT /evidence="ECO:0007829|PDB:6PCZ"
FT TURN 287..289
FT /evidence="ECO:0007829|PDB:6PCZ"
FT HELIX 296..299
FT /evidence="ECO:0007829|PDB:6PCZ"
FT HELIX 304..306
FT /evidence="ECO:0007829|PDB:6PCZ"
FT HELIX 308..314
FT /evidence="ECO:0007829|PDB:6PCZ"
FT STRAND 318..325
FT /evidence="ECO:0007829|PDB:6PCZ"
FT HELIX 329..331
FT /evidence="ECO:0007829|PDB:6PCZ"
FT HELIX 332..338
FT /evidence="ECO:0007829|PDB:6PCZ"
FT STRAND 354..357
FT /evidence="ECO:0007829|PDB:6PCZ"
FT STRAND 363..365
FT /evidence="ECO:0007829|PDB:6PCZ"
FT HELIX 367..379
FT /evidence="ECO:0007829|PDB:6PCZ"
FT STRAND 386..392
FT /evidence="ECO:0007829|PDB:6PCZ"
FT TURN 402..404
FT /evidence="ECO:0007829|PDB:6PCZ"
FT HELIX 410..431
FT /evidence="ECO:0007829|PDB:6PCZ"
FT STRAND 435..441
FT /evidence="ECO:0007829|PDB:6PCZ"
FT STRAND 451..453
FT /evidence="ECO:0007829|PDB:6PCZ"
FT HELIX 463..466
FT /evidence="ECO:0007829|PDB:6PCZ"
FT STRAND 467..476
FT /evidence="ECO:0007829|PDB:6PCZ"
FT STRAND 486..488
FT /evidence="ECO:0007829|PDB:6PCZ"
FT HELIX 494..504
FT /evidence="ECO:0007829|PDB:6PCZ"
FT HELIX 508..510
FT /evidence="ECO:0007829|PDB:6PCZ"
FT HELIX 517..521
FT /evidence="ECO:0007829|PDB:6PCZ"
FT STRAND 531..533
FT /evidence="ECO:0007829|PDB:6PCZ"
FT STRAND 536..541
FT /evidence="ECO:0007829|PDB:6PCZ"
FT STRAND 544..549
FT /evidence="ECO:0007829|PDB:6PCZ"
FT STRAND 554..556
FT /evidence="ECO:0007829|PDB:6PCZ"
SQ SEQUENCE 559 AA; 62032 MW; 7528D897B0E3E4E9 CRC64;
MTQFTQNTAM PSSLWQYWRG LSGWNFYFLV KFGLLWAGYL NFHPLLNLVF AAFLLMPLPR
YSLHRLRHWI ALPIGFALFW HDTWLPGPES IMSQGSQVAG FSTDYLIDLV TRFINWQMIG
AIFVLLVAWL FLSQWIRITV FVVAILLWLN VLTLAGPSFS LWPAGQPTTT VTTTGGNAAA
TVAATGGAPV VGDMPAQTAP PTTANLNAWL NNFYNAEAKR KSTFPSSLPA DAQPFELLVI
NICSLSWSDI EAAGLMSHPL WSHFDIEFKN FNSATSYSGP AAIRLLRASC GQTSHTNLYQ
PANNDCYLFD NLSKLGFTQH LMMGHNGQFG GFLKEVRENG GMQSELMDQT NLPVILLGFD
GSPVYDDTAV LNRWLDVTEK DKNSRSATFY NTLPLHDGNH YPGVSKTADY KARAQKFFDE
LDAFFTELEK SGRKVMVVVV PEHGGALKGD RMQVSGLRDI PSPSITDVPV GVKFFGMKAP
HQGAPIVIEQ PSSFLAISDL VVRVLDGKIF TEDNVDWKKL TSGLPQTAPV SENSNAVVIQ
YQDKPYVRLN GGDWVPYPQ
