RUTF_METED
ID RUTF_METED Reviewed; 205 AA.
AC C7CM32;
DT 11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT 22-SEP-2009, sequence version 1.
DT 25-MAY-2022, entry version 55.
DE RecName: Full=FMN reductase (NADH) RutF {ECO:0000255|HAMAP-Rule:MF_00833};
DE EC=1.5.1.42 {ECO:0000255|HAMAP-Rule:MF_00833};
DE AltName: Full=FMN reductase {ECO:0000255|HAMAP-Rule:MF_00833};
DE AltName: Full=NADH-flavin reductase RutF {ECO:0000255|HAMAP-Rule:MF_00833};
DE AltName: Full=NADH:flavin oxidoreductase {ECO:0000255|HAMAP-Rule:MF_00833};
GN Name=rutF {ECO:0000255|HAMAP-Rule:MF_00833}; OrderedLocusNames=METDI2408;
OS Methylorubrum extorquens (strain DSM 6343 / CIP 106787 / DM4)
OS (Methylobacterium extorquens).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Methylobacteriaceae; Methylorubrum.
OX NCBI_TaxID=661410;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 6343 / CIP 106787 / DM4;
RX PubMed=19440302; DOI=10.1371/journal.pone.0005584;
RA Vuilleumier S., Chistoserdova L., Lee M.-C., Bringel F., Lajus A., Zhou Y.,
RA Gourion B., Barbe V., Chang J., Cruveiller S., Dossat C., Gillett W.,
RA Gruffaz C., Haugen E., Hourcade E., Levy R., Mangenot S., Muller E.,
RA Nadalig T., Pagni M., Penny C., Peyraud R., Robinson D.G., Roche D.,
RA Rouy Z., Saenampechek C., Salvignol G., Vallenet D., Wu Z., Marx C.J.,
RA Vorholt J.A., Olson M.V., Kaul R., Weissenbach J., Medigue C.,
RA Lidstrom M.E.;
RT "Methylobacterium genome sequences: a reference blueprint to investigate
RT microbial metabolism of C1 compounds from natural and industrial sources.";
RL PLoS ONE 4:E5584-E5584(2009).
CC -!- FUNCTION: Catalyzes the reduction of FMN to FMNH2 which is used to
CC reduce pyrimidine by RutA via the Rut pathway. {ECO:0000255|HAMAP-
CC Rule:MF_00833}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=FMNH2 + NAD(+) = FMN + 2 H(+) + NADH; Xref=Rhea:RHEA:21620,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57618,
CC ChEBI:CHEBI:57945, ChEBI:CHEBI:58210; EC=1.5.1.42;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00833};
CC -!- SIMILARITY: Belongs to the non-flavoprotein flavin reductase family.
CC RutF subfamily. {ECO:0000255|HAMAP-Rule:MF_00833}.
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DR EMBL; FP103042; CAX24010.1; -; Genomic_DNA.
DR AlphaFoldDB; C7CM32; -.
DR SMR; C7CM32; -.
DR EnsemblBacteria; CAX24010; CAX24010; METD_I2408.
DR KEGG; mdi:METDI2408; -.
DR HOGENOM; CLU_059021_2_2_5; -.
DR OMA; CINVLPA; -.
DR Proteomes; UP000008070; Chromosome.
DR GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR GO; GO:0052874; F:FMN reductase (NADH) activity; IEA:RHEA.
DR GO; GO:0008752; F:FMN reductase activity; IEA:InterPro.
DR GO; GO:0042602; F:riboflavin reductase (NADPH) activity; IEA:UniProtKB-UniRule.
DR GO; GO:0019740; P:nitrogen utilization; IEA:UniProtKB-UniRule.
DR GO; GO:0006212; P:uracil catabolic process; IEA:UniProtKB-UniRule.
DR Gene3D; 2.30.110.10; -; 1.
DR HAMAP; MF_00833; RutF; 1.
DR InterPro; IPR002563; Flavin_Rdtase-like_dom.
DR InterPro; IPR019917; RutF.
DR InterPro; IPR012349; Split_barrel_FMN-bd.
DR Pfam; PF01613; Flavin_Reduct; 1.
DR SMART; SM00903; Flavin_Reduct; 1.
DR TIGRFAMs; TIGR03615; RutF; 1.
PE 3: Inferred from homology;
KW Flavoprotein; FMN; NAD; Oxidoreductase.
FT CHAIN 1..205
FT /note="FMN reductase (NADH) RutF"
FT /id="PRO_0000403034"
FT REGION 171..205
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 205 AA; 21054 MW; BAD2C8D2DB302A32 CRC64;
MTAFETESES AAVPVDAAAY REAMSRLASA VHLITTDGPG GRAGFTASAV CSVSDAPPTL
LVCINRASSA YAALTQNGTL CVNTLGEGHE TVASLFGGRT PVDERFAAGT WRRLPSGAPA
LTDALVSFDC RIVGRHAVGS HDVLYCAVEA VAASGQADAL LYSERRYRTL PRAPRSGSAP
AEPARAARAL GARPAEGPAL ALRSA
