ID   RUTF_VARPS              Reviewed;         180 AA.
AC   C5CN84;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 1.
DT   25-MAY-2022, entry version 58.
DE   RecName: Full=FMN reductase (NADH) RutF {ECO:0000255|HAMAP-Rule:MF_00833};
DE            EC=1.5.1.42 {ECO:0000255|HAMAP-Rule:MF_00833};
DE   AltName: Full=FMN reductase {ECO:0000255|HAMAP-Rule:MF_00833};
DE   AltName: Full=NADH-flavin reductase RutF {ECO:0000255|HAMAP-Rule:MF_00833};
DE   AltName: Full=NADH:flavin oxidoreductase {ECO:0000255|HAMAP-Rule:MF_00833};
GN   Name=rutF {ECO:0000255|HAMAP-Rule:MF_00833}; OrderedLocusNames=Vapar_4843;
OS   Variovorax paradoxus (strain S110).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Variovorax.
OX   NCBI_TaxID=543728;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=S110;
RX   PubMed=21183664; DOI=10.1128/jb.00925-10;
RA   Han J.I., Choi H.K., Lee S.W., Orwin P.M., Kim J., Laroe S.L., Kim T.G.,
RA   O'Neil J., Leadbetter J.R., Lee S.Y., Hur C.G., Spain J.C.,
RA   Ovchinnikova G., Goodwin L., Han C.;
RT   "Complete genome sequence of the metabolically versatile plant growth-
RT   promoting endophyte, Variovorax paradoxus S110.";
RL   J. Bacteriol. 193:1183-1190(2011).
CC   -!- FUNCTION: Catalyzes the reduction of FMN to FMNH2 which is used to
CC       reduce pyrimidine by RutA via the Rut pathway. {ECO:0000255|HAMAP-
CC       Rule:MF_00833}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=FMNH2 + NAD(+) = FMN + 2 H(+) + NADH; Xref=Rhea:RHEA:21620,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57618,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:58210; EC=1.5.1.42;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00833};
CC   -!- SIMILARITY: Belongs to the non-flavoprotein flavin reductase family.
CC       RutF subfamily. {ECO:0000255|HAMAP-Rule:MF_00833}.
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DR   EMBL; CP001635; ACS21447.1; -; Genomic_DNA.
DR   RefSeq; WP_015867603.1; NC_012791.1.
DR   AlphaFoldDB; C5CN84; -.
DR   SMR; C5CN84; -.
DR   STRING; 543728.Vapar_4843; -.
DR   EnsemblBacteria; ACS21447; ACS21447; Vapar_4843.
DR   GeneID; 45059077; -.
DR   KEGG; vap:Vapar_4843; -.
DR   eggNOG; COG1853; Bacteria.
DR   HOGENOM; CLU_059021_2_2_4; -.
DR   OMA; WFDRGYH; -.
DR   OrthoDB; 1681849at2; -.
DR   GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR   GO; GO:0052874; F:FMN reductase (NADH) activity; IEA:RHEA.
DR   GO; GO:0008752; F:FMN reductase activity; IEA:InterPro.
DR   GO; GO:0042602; F:riboflavin reductase (NADPH) activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019740; P:nitrogen utilization; IEA:UniProtKB-UniRule.
DR   GO; GO:0006212; P:uracil catabolic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.30.110.10; -; 1.
DR   HAMAP; MF_00833; RutF; 1.
DR   InterPro; IPR002563; Flavin_Rdtase-like_dom.
DR   InterPro; IPR019917; RutF.
DR   InterPro; IPR012349; Split_barrel_FMN-bd.
DR   Pfam; PF01613; Flavin_Reduct; 1.
DR   SMART; SM00903; Flavin_Reduct; 1.
DR   TIGRFAMs; TIGR03615; RutF; 1.
PE   3: Inferred from homology;
KW   Flavoprotein; FMN; NAD; Oxidoreductase.
FT   CHAIN           1..180
FT                   /note="FMN reductase (NADH) RutF"
FT                   /id="PRO_0000403049"
SQ   SEQUENCE   180 AA;  18641 MW;  5194FC503DFE846E CRC64;
     MLPNAMASNH VMPSGAPPVL PKADYRNAMA RLGAAVNIIT TDGPAGRAGF TASAVCSVTD
     EPPMLLVCLN RSASVYPAFT ANGVLCVNVL AAGHQALSGL FGGKTPMDER FAAGRWSRKA
     TGSPVLEDAA ASFDCRVVQA TSAGTHDVLF CEALAIAIGG AAQSLIYFDR RYHEIAAPPH