BCSQP_ECOLI
ID BCSQP_ECOLI Reviewed; 242 AA.
AC P37655;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2017, sequence version 4.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Putative cellulose biosynthesis protein BcsQ;
GN Name=bcsQ; Synonyms=yhjQ; OrderedLocusNames=b3534;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8041620; DOI=10.1093/nar/22.13.2576;
RA Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT "Analysis of the Escherichia coli genome. V. DNA sequence of the region
RT from 76.0 to 81.5 minutes.";
RL Nucleic Acids Res. 22:2576-2586(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP IDENTIFICATION OF MUTATION THAT GENERATES PSEUDOGENE, AND RESTORATION OF
RP THE READING FRAME AND CELLULOSE PRODUCTION.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=24097954; DOI=10.1128/jb.00946-13;
RA Serra D.O., Richter A.M., Hengge R.;
RT "Cellulose as an architectural element in spatially structured Escherichia
RT coli biofilms.";
RL J. Bacteriol. 195:5540-5554(2013).
CC -!- FUNCTION: A C-terminal pseudogene fragment of BcsQ, which has a
CC premature stop codon at position 6 of the intact protein. The mutation
CC prevents cellulose synthesis, and has polar effects on transcription of
CC downstream gene bcsA and probably also bcsB, bcsZ and bcsC
CC (PubMed:24097954). When the reading frame is restored cellulose
CC biosynthesis is also restored, see (AC P0DP92) for an intact protein.
CC May play a role in subcellular localization of an active cellulose
CC biosynthesis apparatus at the bacterial cell pole (By similarity).
CC {ECO:0000250|UniProtKB:P0DP92, ECO:0000305|PubMed:24097954}.
CC -!- INDUCTION: Part of the yhjR-bcsQABZC operon (PubMed:24097954).
CC {ECO:0000269|PubMed:24097954}.
CC -!- MISCELLANEOUS: Cellulose production is abolished in E.coli K12 /MG1655
CC and W3110 due to a premature stop codon in this gene. The sequence
CC given here starts on a GTG codon 6 nucleotides downstream of the
CC premature stop codon. {ECO:0000269|PubMed:24097954}.
CC -!- SIMILARITY: Belongs to the BcsQ family. {ECO:0000305}.
CC -!- CAUTION: Could be the product of a pseudogene. The protein is missing 8
CC N-terminal amino acids due to a premature stop codon; when the reading
CC frame is restored strain W3110 synthesizes cellulose. See (AC P0DP92)
CC for a functional intact protein. {ECO:0000305|PubMed:24097954}.
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DR EMBL; U00039; AAB18512.1; -; Genomic_DNA.
DR EMBL; U00096; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; S47756; S47756.
DR RefSeq; WP_011310329.1; NZ_CP047127.1.
DR AlphaFoldDB; P37655; -.
DR SMR; P37655; -.
DR DIP; DIP-12388N; -.
DR IntAct; P37655; 2.
DR PRIDE; P37655; -.
DR EchoBASE; EB2170; -.
DR InParanoid; P37655; -.
DR PhylomeDB; P37655; -.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0009898; C:cytoplasmic side of plasma membrane; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IBA:GO_Central.
DR GO; GO:0016887; F:ATP hydrolysis activity; IBA:GO_Central.
DR GO; GO:0051782; P:negative regulation of cell division; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR017746; Cellulose_synthase_operon_BcsQ.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF06564; CBP_BcsQ; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR03371; cellulose_yhjQ; 1.
PE 5: Uncertain;
KW ATP-binding; Nucleotide-binding; Reference proteome.
FT CHAIN 1..242
FT /note="Putative cellulose biosynthesis protein BcsQ"
FT /id="PRO_0000169575"
FT BINDING 1..8
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 242 AA; 27140 MW; C4DD72EC40E124A9 CRC64;
MRGGVGTTTI TAALAWSLQM LGENVLVVDA CPDNLLRLSF NVDFTHRQGW ARAMLDGQDW
RDAGLRYTSQ LDLLPFGQLS IEEQENPQHW QTRLSDICSG LQQLKASGRY QWILIDLPRD
ASQITHQLLS LCDHSLAIVN VDANCHIRLH QQALPDGAHI LINDFRIGSQ VQDDIYQLWL
QSQRRLLPML IHRDEAMAEC LAAKQPVGEY RSDALAAEEI LTLANWCLLN YSGLKTPVGS
AS