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BDH2_BOVIN
ID   BDH2_BOVIN              Reviewed;         245 AA.
AC   Q3T046;
DT   25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2005, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=3-hydroxybutyrate dehydrogenase type 2;
DE            EC=1.1.1.30 {ECO:0000250|UniProtKB:Q9BUT1};
DE   AltName: Full=4-oxo-L-proline reductase {ECO:0000250|UniProtKB:Q9BUT1};
DE            EC=1.1.1.104 {ECO:0000250|UniProtKB:D4A1J4, ECO:0000250|UniProtKB:Q9BUT1};
DE   AltName: Full=Dehydrogenase/reductase SDR family member 6;
DE   AltName: Full=R-beta-hydroxybutyrate dehydrogenase;
GN   Name=BDH2; Synonyms=DHRS6;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Testis;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: NAD(H)-dependent dehydrogenase/reductase with a preference
CC       for cyclic substrates (By similarity). Catalyzes stereoselective
CC       conversion of 4-oxo-L-proline to cis-4-hydroxy-L-proline, likely a
CC       detoxification mechanism for ketoprolines (By similarity). Mediates the
CC       formation of 2,5-dihydroxybenzoate (2,5-DHBA), a siderophore that
CC       chelates free cytoplasmic iron and associates with LCN2, thereby
CC       regulating iron transport and homeostasis while protecting cells
CC       against free radical-induced oxidative stress. The iron-siderophore
CC       complex is imported into mitochondria, providing an iron source for
CC       mitochondrial metabolic processes in particular heme synthesis (By
CC       similarity). May act as a 3-hydroxybutyrate dehydrogenase (By
CC       similarity). {ECO:0000250|UniProtKB:Q8JZV9,
CC       ECO:0000250|UniProtKB:Q9BUT1}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=cis-4-hydroxy-L-proline + NAD(+) = 4-oxo-L-proline + H(+) +
CC         NADH; Xref=Rhea:RHEA:13601, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:63727, ChEBI:CHEBI:84813;
CC         EC=1.1.1.104; Evidence={ECO:0000250|UniProtKB:Q9BUT1};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:13603;
CC         Evidence={ECO:0000250|UniProtKB:Q9BUT1};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(R)-3-hydroxybutanoate + NAD(+) = acetoacetate + H(+) + NADH;
CC         Xref=Rhea:RHEA:20521, ChEBI:CHEBI:10983, ChEBI:CHEBI:13705,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.30;
CC         Evidence={ECO:0000250|UniProtKB:Q9BUT1};
CC   -!- PATHWAY: Amino-acid metabolism. {ECO:0000250|UniProtKB:Q9BUT1}.
CC   -!- PATHWAY: Siderophore biosynthesis. {ECO:0000250|UniProtKB:Q8JZV9}.
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250|UniProtKB:Q9BUT1}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9BUT1}.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
CC   -!- CAUTION: Postulated to act as a 3-hydroxybutyrate dehydrogenase,
CC       however its contribution to ketone body formation appears to be
CC       physiologically irrelevant since it has very low affinity for the
CC       substrate. {ECO:0000250|UniProtKB:Q8JZV9,
CC       ECO:0000250|UniProtKB:Q9BUT1}.
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DR   EMBL; BC102567; AAI02568.1; -; mRNA.
DR   RefSeq; NP_001029660.1; NM_001034488.2.
DR   AlphaFoldDB; Q3T046; -.
DR   SMR; Q3T046; -.
DR   STRING; 9913.ENSBTAP00000003277; -.
DR   PaxDb; Q3T046; -.
DR   PeptideAtlas; Q3T046; -.
DR   GeneID; 515321; -.
DR   KEGG; bta:515321; -.
DR   CTD; 56898; -.
DR   eggNOG; KOG0725; Eukaryota.
DR   InParanoid; Q3T046; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003858; F:3-hydroxybutyrate dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016628; F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor; ISS:UniProtKB.
DR   GO; GO:0042168; P:heme metabolic process; ISS:UniProtKB.
DR   GO; GO:0055072; P:iron ion homeostasis; ISS:UniProtKB.
DR   GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0019290; P:siderophore biosynthetic process; ISS:UniProtKB.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Lipid metabolism; NAD; Oxidoreductase; Reference proteome.
FT   CHAIN           1..245
FT                   /note="3-hydroxybutyrate dehydrogenase type 2"
FT                   /id="PRO_0000247551"
FT   ACT_SITE        147
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT   BINDING         16..18
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:Q9BUT1"
FT   BINDING         37
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:Q9BUT1"
FT   BINDING         58
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:Q9BUT1"
FT   BINDING         144
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         151
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:Q9BUT1"
FT   BINDING         180..184
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:Q9BUT1"
FT   BINDING         188
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         205
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   245 AA;  26662 MW;  A846933ACBA6F83C CRC64;
     MGRLDGKVIV LTAAAQGIGR AAALAFAKEG AKVIATDIND SKLQELDKYP GIHTRVLDVT
     KKKQIDQFAN DIERLDVLFN VAGFVHHGTI LDCEETDWDF SMNLNVRSMY LMIKAFLPKM
     MAQKSGNIIN MSSVASSIKG VVNRCVYSTT KAAVIGLTKS VAADFIQQGI RCNCVCPGTV
     DTPSLQERIQ ARPNPEEALS DFLKRQKTGR FATAEEVALL CVYLASDESA YITGNPVIID
     GGWSL
 
 
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