BDHA_CUPNH
ID BDHA_CUPNH Reviewed; 258 AA.
AC Q9X6U2; Q0KBZ7;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=D-beta-hydroxybutyrate dehydrogenase;
DE Short=BDH;
DE EC=1.1.1.30;
DE AltName: Full=3-hydroxybutyrate dehydrogenase;
DE Short=3-HBDH;
GN Name=hbdH1; OrderedLocusNames=H16_A1334;
OS Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442
OS / H16 / Stanier 337) (Ralstonia eutropha).
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Cupriavidus.
OX NCBI_TaxID=381666;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Kim J.W., Kang D.G., Rha E.G.;
RT "Cloning and sequencing of the gene for beta-hydroxybutyrate dehydrogenase
RT from Ralstonia eutropha.";
RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337;
RX PubMed=16964242; DOI=10.1038/nbt1244;
RA Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R.,
RA Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I.,
RA Gottschalk G., Steinbuechel A., Friedrich B., Bowien B.;
RT "Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia
RT eutropha H16.";
RL Nat. Biotechnol. 24:1257-1262(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(R)-3-hydroxybutanoate + NAD(+) = acetoacetate + H(+) + NADH;
CC Xref=Rhea:RHEA:20521, ChEBI:CHEBI:10983, ChEBI:CHEBI:13705,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.30;
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. {ECO:0000305}.
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DR EMBL; AF145230; AAD33952.1; -; Genomic_DNA.
DR EMBL; AM260479; CAJ92474.1; -; Genomic_DNA.
DR RefSeq; WP_010813107.1; NZ_CP039287.1.
DR AlphaFoldDB; Q9X6U2; -.
DR SMR; Q9X6U2; -.
DR STRING; 381666.H16_A1334; -.
DR EnsemblBacteria; CAJ92474; CAJ92474; H16_A1334.
DR GeneID; 57643432; -.
DR KEGG; reh:H16_A1334; -.
DR eggNOG; COG1028; Bacteria.
DR HOGENOM; CLU_010194_1_0_4; -.
DR OMA; QHGKIDN; -.
DR OrthoDB; 1094766at2; -.
DR Proteomes; UP000008210; Chromosome 1.
DR GO; GO:0003858; F:3-hydroxybutyrate dehydrogenase activity; IEA:UniProtKB-EC.
DR InterPro; IPR011294; 3-OHbutyrate_DH.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR InterPro; IPR002347; SDR_fam.
DR Pfam; PF00106; adh_short; 1.
DR PRINTS; PR00081; GDHRDH.
DR PRINTS; PR00080; SDRFAMILY.
DR SUPFAM; SSF51735; SSF51735; 1.
DR TIGRFAMs; TIGR01963; PHB_DH; 1.
DR PROSITE; PS00061; ADH_SHORT; 1.
PE 3: Inferred from homology;
KW NAD; Oxidoreductase; Reference proteome.
FT CHAIN 1..258
FT /note="D-beta-hydroxybutyrate dehydrogenase"
FT /id="PRO_0000054527"
FT ACT_SITE 153
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT BINDING 8..32
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 140
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT CONFLICT 236
FT /note="L -> Q (in Ref. 1; AAD33952)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 258 AA; 26999 MW; 0B9A06D6CD97EF35 CRC64;
MLNGKTALVT GSTSGIGLGI AKALAAQGAN IIVNGFGDAD AAKAEIAQAG QGIRVGYHGA
DMSKAAEIED MMRYAQSDFG GADILVNNAG IQHVAAIEDF PPERWDAIIA INLTSAFHTT
RLALPGMKQK DWGRIINVAS THGLVASAQK SAYVAAKHGI VGFTKVTALE TAQTGVTANA
ICPGWVLTPL VQKQVEARAQ KEGIPVEQAK RELVLEKQPS GQFVTPDELG ALAVFLSSEA
ARQVRGAIWN MDGGWVAQ
