ID   14KL_BRUA2              Reviewed;         147 AA.
AC   Q2YKY9; Q44701; Q578L8;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   07-FEB-2006, sequence version 1.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=Lectin-like protein BA14k;
DE   Flags: Precursor;
GN   OrderedLocusNames=BAB2_0505;
OS   Brucella abortus (strain 2308).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=359391;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND IMMUNOGENICITY.
RX   PubMed=9673296; DOI=10.1128/iai.66.8.4000-4003.1998;
RA   Chirhart-Gilleland R.L., Kovach M.E., Elzer P.H., Jennings S.R.,
RA   Roop R.M. II;
RT   "Identification and characterization of a 14-kilodalton Brucella abortus
RT   protein reactive with antibodies from naturally and experimentally infected
RT   hosts and T lymphocytes from experimentally infected BALB/c mice.";
RL   Infect. Immun. 66:4000-4003(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=2308;
RX   PubMed=16299333; DOI=10.1128/iai.73.12.8353-8361.2005;
RA   Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A.,
RA   Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E.;
RT   "Whole-genome analyses of speciation events in pathogenic Brucellae.";
RL   Infect. Immun. 73:8353-8361(2005).
RN   [3]
RP   LECTIN-LIKE ACTIVITY, POSSIBLE FUNCTION IN LPS SYNTHESIS, AND DISRUPTION
RP   PHENOTYPE.
RX   PubMed=16368972; DOI=10.1128/iai.74.1.183-191.2006;
RA   Vemulapalli T.H., Vemulapalli R., Schurig G.G., Boyle S.M.,
RA   Sriranganathan N.;
RT   "Role in virulence of a Brucella abortus protein exhibiting lectin-like
RT   activity.";
RL   Infect. Immun. 74:183-191(2006).
CC   -!- FUNCTION: Has immunoglobulin-binding and hemagglutination properties,
CC       and can bind to mannose. Essential for virulence. May be involved in
CC       LPS biosynthesis or polysaccharide transport.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: Rough phenotype, with an aberrant O-antigen
CC       profile. Mutants exhibit a reduced ability to colonize mouse spleens
CC       but are still capable of producing a persistent infection, albeit with
CC       a low bacterial burden. {ECO:0000269|PubMed:16368972}.
CC   -!- MISCELLANEOUS: Strongly immunoreactive, inducing both humoral and
CC       cellular immune responses in hosts during the course of infection.
CC   -!- SIMILARITY: Belongs to the BA14k family. {ECO:0000305}.
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DR   EMBL; U62541; AAC31618.1; -; Genomic_DNA.
DR   EMBL; AM040265; CAJ12671.1; -; Genomic_DNA.
DR   RefSeq; WP_002965908.1; NZ_KN046823.1.
DR   AlphaFoldDB; Q2YKY9; -.
DR   STRING; 359391.BAB2_0505; -.
DR   EnsemblBacteria; CAJ12671; CAJ12671; BAB2_0505.
DR   GeneID; 45126066; -.
DR   GeneID; 55592388; -.
DR   KEGG; bmf:BAB2_0505; -.
DR   PATRIC; fig|359391.11.peg.2695; -.
DR   HOGENOM; CLU_095992_0_0_5; -.
DR   OMA; HVAWCAN; -.
DR   PhylomeDB; Q2YKY9; -.
DR   Proteomes; UP000002719; Chromosome II.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:UniProtKB-KW.
DR   InterPro; IPR012413; BA14K.
DR   Pfam; PF07886; BA14K; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Lectin; Membrane; Reference proteome; Signal; Transmembrane;
KW   Transmembrane helix; Virulence.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   CHAIN           27..147
FT                   /note="Lectin-like protein BA14k"
FT                   /id="PRO_0000361296"
FT   TRANSMEM        80..100
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   147 AA;  16823 MW;  7CEE6F1FB2BB38DB CRC64;
     MNSFRKTCAG ALALIFGATS IVPTVAAPMN MDRPAINQNV IQARAHYRPQ NYNRGHRPGY
     WHGHRGYRHY RHGYRRHNDG WWYPLAAFGA GAIIGGAISQ PRPVYRAPAG SPHVQWCYSR
     YKSYRASDNT FQPYNGPRKQ CRSPYSR