BDHA_RHIME
ID BDHA_RHIME Reviewed; 258 AA.
AC O86034;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=D-beta-hydroxybutyrate dehydrogenase;
DE Short=BDH;
DE EC=1.1.1.30;
DE AltName: Full=3-hydroxybutyrate dehydrogenase;
DE Short=3-HBDH;
GN Name=bdhA; OrderedLocusNames=RB1136; ORFNames=SMb21010;
OS Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS meliloti).
OG Plasmid pSymB (megaplasmid 2).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=266834;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=SU47 / 1021;
RX PubMed=9922248; DOI=10.1128/jb.181.3.849-857.1999;
RA Aneja P., Charles T.C.;
RT "Poly-3-hydroxybutyrate degradation in Rhizobium (Sinorhizobium) meliloti:
RT isolation and characterization of a gene encoding 3-hydroxybutyrate
RT dehydrogenase.";
RL J. Bacteriol. 181:849-857(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11481431; DOI=10.1073/pnas.161294698;
RA Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J.,
RA Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A.;
RT "The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing
RT endosymbiont Sinorhizobium meliloti.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11474104; DOI=10.1126/science.1060966;
RA Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA Wong K., Yeh K.-C., Batut J.;
RT "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL Science 293:668-672(2001).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(R)-3-hydroxybutanoate + NAD(+) = acetoacetate + H(+) + NADH;
CC Xref=Rhea:RHEA:20521, ChEBI:CHEBI:10983, ChEBI:CHEBI:13705,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.30;
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. {ECO:0000305}.
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DR EMBL; AF080548; AAD12733.1; -; Genomic_DNA.
DR EMBL; AL591985; CAC49536.1; -; Genomic_DNA.
DR PIR; H95983; H95983.
DR RefSeq; NP_437676.1; NC_003078.1.
DR RefSeq; WP_010975968.1; NC_003078.1.
DR PDB; 3V2H; X-ray; 3.00 A; A/B=1-258.
DR PDBsum; 3V2H; -.
DR AlphaFoldDB; O86034; -.
DR SMR; O86034; -.
DR STRING; 266834.SM_b21010; -.
DR EnsemblBacteria; CAC49536; CAC49536; SM_b21010.
DR GeneID; 61601087; -.
DR KEGG; sme:SM_b21010; -.
DR PATRIC; fig|266834.11.peg.6065; -.
DR eggNOG; COG1028; Bacteria.
DR HOGENOM; CLU_010194_1_0_5; -.
DR OMA; QHGKIDN; -.
DR Proteomes; UP000001976; Plasmid pSymB.
DR GO; GO:0003858; F:3-hydroxybutyrate dehydrogenase activity; IEA:UniProtKB-EC.
DR InterPro; IPR011294; 3-OHbutyrate_DH.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR InterPro; IPR002347; SDR_fam.
DR PRINTS; PR00081; GDHRDH.
DR PRINTS; PR00080; SDRFAMILY.
DR SUPFAM; SSF51735; SSF51735; 1.
DR TIGRFAMs; TIGR01963; PHB_DH; 1.
DR PROSITE; PS00061; ADH_SHORT; 1.
PE 1: Evidence at protein level;
KW 3D-structure; NAD; Oxidoreductase; Plasmid; Reference proteome.
FT CHAIN 1..258
FT /note="D-beta-hydroxybutyrate dehydrogenase"
FT /id="PRO_0000054528"
FT ACT_SITE 153
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT BINDING 6..30
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 140
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT STRAND 4..8
FT /evidence="ECO:0007829|PDB:3V2H"
FT HELIX 13..24
FT /evidence="ECO:0007829|PDB:3V2H"
FT STRAND 28..32
FT /evidence="ECO:0007829|PDB:3V2H"
FT HELIX 37..48
FT /evidence="ECO:0007829|PDB:3V2H"
FT STRAND 55..58
FT /evidence="ECO:0007829|PDB:3V2H"
FT HELIX 65..78
FT /evidence="ECO:0007829|PDB:3V2H"
FT STRAND 79..81
FT /evidence="ECO:0007829|PDB:3V2H"
FT STRAND 83..87
FT /evidence="ECO:0007829|PDB:3V2H"
FT HELIX 97..99
FT /evidence="ECO:0007829|PDB:3V2H"
FT HELIX 102..112
FT /evidence="ECO:0007829|PDB:3V2H"
FT HELIX 114..130
FT /evidence="ECO:0007829|PDB:3V2H"
FT STRAND 133..138
FT /evidence="ECO:0007829|PDB:3V2H"
FT HELIX 141..143
FT /evidence="ECO:0007829|PDB:3V2H"
FT HELIX 151..171
FT /evidence="ECO:0007829|PDB:3V2H"
FT HELIX 172..174
FT /evidence="ECO:0007829|PDB:3V2H"
FT STRAND 176..183
FT /evidence="ECO:0007829|PDB:3V2H"
FT HELIX 226..237
FT /evidence="ECO:0007829|PDB:3V2H"
FT HELIX 240..243
FT /evidence="ECO:0007829|PDB:3V2H"
FT STRAND 248..252
FT /evidence="ECO:0007829|PDB:3V2H"
FT HELIX 255..257
FT /evidence="ECO:0007829|PDB:3V2H"
SQ SEQUENCE 258 AA; 27179 MW; ACE9D388A4F0D0BA CRC64;
MTKTAVITGS TSGIGLAIAR TLAKAGANIV LNGFGAPDEI RTVTDEVAGL SSGTVLHHPA
DMTKPSEIAD MMAMVADRFG GADILVNNAG VQFVEKIEDF PVEQWDRIIA VNLSSSFHTI
RGAIPPMKKK GWGRIINIAS AHGLVASPFK SAYVAAKHGI MGLTKTVALE VAESGVTVNS
ICPGYVLTPL VEKQIPDQAR TRGITEEQVI NEVMLKGQPT KKFITVEQVA SLALYLAGDD
AAQITGTHVS MDGGWTAQ
