RUVB_CAMJE
ID RUVB_CAMJE Reviewed; 335 AA.
AC Q9PMT7; Q0P8P7;
DT 03-APR-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Holliday junction ATP-dependent DNA helicase RuvB {ECO:0000255|HAMAP-Rule:MF_00016};
DE EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_00016};
GN Name=ruvB {ECO:0000255|HAMAP-Rule:MF_00016}; OrderedLocusNames=Cj1362;
OS Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC
OS 11168).
OC Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC Campylobacteraceae; Campylobacter.
OX NCBI_TaxID=192222;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700819 / NCTC 11168;
RX PubMed=10688204; DOI=10.1038/35001088;
RA Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M.,
RA Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S.,
RA Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A.,
RA Rajandream M.A., Rutherford K.M., van Vliet A.H.M., Whitehead S.,
RA Barrell B.G.;
RT "The genome sequence of the food-borne pathogen Campylobacter jejuni
RT reveals hypervariable sequences.";
RL Nature 403:665-668(2000).
CC -!- FUNCTION: The RuvA-RuvB complex in the presence of ATP renatures
CC cruciform structure in supercoiled DNA with palindromic sequence,
CC indicating that it may promote strand exchange reactions in homologous
CC recombination. RuvAB is a helicase that mediates the Holliday junction
CC migration by localized denaturation and reannealing.
CC {ECO:0000255|HAMAP-Rule:MF_00016}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00016};
CC -!- SUBUNIT: Forms a complex with RuvA. {ECO:0000255|HAMAP-Rule:MF_00016}.
CC -!- SIMILARITY: Belongs to the RuvB family. {ECO:0000255|HAMAP-
CC Rule:MF_00016}.
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DR EMBL; AL111168; CAL35474.1; -; Genomic_DNA.
DR PIR; G81280; G81280.
DR RefSeq; WP_002856258.1; NC_002163.1.
DR RefSeq; YP_002344750.1; NC_002163.1.
DR PDB; 3PFI; X-ray; 2.69 A; A/B=1-335.
DR PDBsum; 3PFI; -.
DR AlphaFoldDB; Q9PMT7; -.
DR SMR; Q9PMT7; -.
DR IntAct; Q9PMT7; 9.
DR STRING; 192222.Cj1362; -.
DR PaxDb; Q9PMT7; -.
DR PRIDE; Q9PMT7; -.
DR EnsemblBacteria; CAL35474; CAL35474; Cj1362.
DR GeneID; 905655; -.
DR KEGG; cje:Cj1362; -.
DR PATRIC; fig|192222.6.peg.1343; -.
DR eggNOG; COG2255; Bacteria.
DR HOGENOM; CLU_055599_1_0_7; -.
DR OMA; IHRMSRP; -.
DR Proteomes; UP000000799; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0009378; F:four-way junction helicase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0009432; P:SOS response; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00016; DNA_helic_RuvB; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR041445; AAA_lid_4.
DR InterPro; IPR004605; DNA_helicase_Holl-junc_RuvB.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR008824; RuvB-like_N.
DR InterPro; IPR008823; RuvB_C.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR42848; PTHR42848; 1.
DR Pfam; PF17864; AAA_lid_4; 1.
DR Pfam; PF05491; RuvB_C; 1.
DR Pfam; PF05496; RuvB_N; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00635; ruvB; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; DNA damage; DNA recombination; DNA repair;
KW Helicase; Hydrolase; Nucleotide-binding; Reference proteome; SOS response.
FT CHAIN 1..335
FT /note="Holliday junction ATP-dependent DNA helicase RuvB"
FT /id="PRO_0000165510"
FT BINDING 59..66
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00016"
FT HELIX 25..27
FT /evidence="ECO:0007829|PDB:3PFI"
FT HELIX 32..47
FT /evidence="ECO:0007829|PDB:3PFI"
FT STRAND 55..58
FT /evidence="ECO:0007829|PDB:3PFI"
FT HELIX 65..75
FT /evidence="ECO:0007829|PDB:3PFI"
FT STRAND 80..84
FT /evidence="ECO:0007829|PDB:3PFI"
FT HELIX 85..87
FT /evidence="ECO:0007829|PDB:3PFI"
FT HELIX 91..99
FT /evidence="ECO:0007829|PDB:3PFI"
FT STRAND 106..110
FT /evidence="ECO:0007829|PDB:3PFI"
FT HELIX 112..114
FT /evidence="ECO:0007829|PDB:3PFI"
FT HELIX 117..128
FT /evidence="ECO:0007829|PDB:3PFI"
FT STRAND 153..159
FT /evidence="ECO:0007829|PDB:3PFI"
FT HELIX 161..163
FT /evidence="ECO:0007829|PDB:3PFI"
FT HELIX 166..169
FT /evidence="ECO:0007829|PDB:3PFI"
FT STRAND 173..177
FT /evidence="ECO:0007829|PDB:3PFI"
FT HELIX 183..196
FT /evidence="ECO:0007829|PDB:3PFI"
FT HELIX 203..211
FT /evidence="ECO:0007829|PDB:3PFI"
FT TURN 212..215
FT /evidence="ECO:0007829|PDB:3PFI"
FT HELIX 217..233
FT /evidence="ECO:0007829|PDB:3PFI"
FT STRAND 237..239
FT /evidence="ECO:0007829|PDB:3PFI"
FT HELIX 241..251
FT /evidence="ECO:0007829|PDB:3PFI"
FT HELIX 260..271
FT /evidence="ECO:0007829|PDB:3PFI"
FT HELIX 279..285
FT /evidence="ECO:0007829|PDB:3PFI"
FT HELIX 290..295
FT /evidence="ECO:0007829|PDB:3PFI"
FT HELIX 298..303
FT /evidence="ECO:0007829|PDB:3PFI"
FT STRAND 306..310
FT /evidence="ECO:0007829|PDB:3PFI"
FT STRAND 313..316
FT /evidence="ECO:0007829|PDB:3PFI"
FT HELIX 318..327
FT /evidence="ECO:0007829|PDB:3PFI"
SQ SEQUENCE 335 AA; 37312 MW; 84C75FBCB75F4441 CRC64;
MDRIVEIEKY SFDETYETSL RPSNFDGYIG QESIKKNLNV FIAAAKKRNE CLDHILFSGP
AGLGKTTLAN IISYEMSANI KTTAAPMIEK SGDLAAILTN LSEGDILFID EIHRLSPAIE
EVLYPAMEDY RLDIIIGSGP AAQTIKIDLP KFTLIGATTR AGMLSNPLRD RFGMQFRLEF
YKDSELALIL QKAALKLNKT CEEKAALEIA KRSRSTPRIA LRLLKRVRDF ADVNDEEIIT
EKRANEALNS LGVNELGFDA MDLRYLELLT AAKQKPIGLA SIAAALSEDE NTIEDVIEPY
LLANGYIERT AKGRIASAKS YSALKLNYEK TLFEE
