RUVB_PSEAE
ID RUVB_PSEAE Reviewed; 352 AA.
AC Q51426; Q9I4Z6;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 11-JAN-2001, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Holliday junction ATP-dependent DNA helicase RuvB {ECO:0000255|HAMAP-Rule:MF_00016};
DE EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_00016};
GN Name=ruvB {ECO:0000255|HAMAP-Rule:MF_00016}; OrderedLocusNames=PA0967;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=8982068; DOI=10.1016/s0378-1119(96)00474-x;
RA Hishida T., Iwasaki H., Ishioka K., Shinagawa H.;
RT "Molecular analysis of the Pseudomonas aeruginosa genes, ruvA, ruvB and
RT ruvC, involved in processing of homologous recombination intermediates.";
RL Gene 182:63-70(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: The RuvA-RuvB complex in the presence of ATP renatures
CC cruciform structure in supercoiled DNA with palindromic sequence,
CC indicating that it may promote strand exchange reactions in homologous
CC recombination. RuvAB is a helicase that mediates the Holliday junction
CC migration by localized denaturation and reannealing.
CC {ECO:0000255|HAMAP-Rule:MF_00016}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00016};
CC -!- SUBUNIT: Forms a complex with RuvA. {ECO:0000255|HAMAP-Rule:MF_00016}.
CC -!- SIMILARITY: Belongs to the RuvB family. {ECO:0000255|HAMAP-
CC Rule:MF_00016}.
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DR EMBL; D83138; BAA11819.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG04356.1; -; Genomic_DNA.
DR PIR; A83525; A83525.
DR RefSeq; NP_249658.1; NC_002516.2.
DR RefSeq; WP_003086123.1; NZ_QZGE01000007.1.
DR PDB; 6BLB; X-ray; 1.88 A; A=1-352.
DR PDBsum; 6BLB; -.
DR AlphaFoldDB; Q51426; -.
DR SMR; Q51426; -.
DR STRING; 287.DR97_970; -.
DR PaxDb; Q51426; -.
DR PRIDE; Q51426; -.
DR EnsemblBacteria; AAG04356; AAG04356; PA0967.
DR GeneID; 882028; -.
DR KEGG; pae:PA0967; -.
DR PATRIC; fig|208964.12.peg.1005; -.
DR PseudoCAP; PA0967; -.
DR HOGENOM; CLU_055599_1_0_6; -.
DR InParanoid; Q51426; -.
DR OMA; IHRMSRP; -.
DR PhylomeDB; Q51426; -.
DR BioCyc; PAER208964:G1FZ6-988-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0009378; F:four-way junction helicase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0071247; P:cellular response to chromate; IMP:PseudoCAP.
DR GO; GO:0072715; P:cellular response to selenite ion; IMP:PseudoCAP.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IMP:PseudoCAP.
DR GO; GO:0009432; P:SOS response; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00016; DNA_helic_RuvB; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR041445; AAA_lid_4.
DR InterPro; IPR004605; DNA_helicase_Holl-junc_RuvB.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR008824; RuvB-like_N.
DR InterPro; IPR008823; RuvB_C.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR42848; PTHR42848; 1.
DR Pfam; PF17864; AAA_lid_4; 1.
DR Pfam; PF05491; RuvB_C; 1.
DR Pfam; PF05496; RuvB_N; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00635; ruvB; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; DNA damage; DNA recombination; DNA repair;
KW Helicase; Hydrolase; Nucleotide-binding; Reference proteome; SOS response.
FT CHAIN 1..352
FT /note="Holliday junction ATP-dependent DNA helicase RuvB"
FT /id="PRO_0000165578"
FT BINDING 63..70
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00016"
FT CONFLICT 146
FT /note="A -> G (in Ref. 1; BAA11819)"
FT /evidence="ECO:0000305"
FT HELIX 29..31
FT /evidence="ECO:0007829|PDB:6BLB"
FT HELIX 36..51
FT /evidence="ECO:0007829|PDB:6BLB"
FT STRAND 59..64
FT /evidence="ECO:0007829|PDB:6BLB"
FT HELIX 69..80
FT /evidence="ECO:0007829|PDB:6BLB"
FT STRAND 84..88
FT /evidence="ECO:0007829|PDB:6BLB"
FT TURN 89..91
FT /evidence="ECO:0007829|PDB:6BLB"
FT HELIX 95..102
FT /evidence="ECO:0007829|PDB:6BLB"
FT STRAND 110..114
FT /evidence="ECO:0007829|PDB:6BLB"
FT HELIX 116..118
FT /evidence="ECO:0007829|PDB:6BLB"
FT HELIX 121..133
FT /evidence="ECO:0007829|PDB:6BLB"
FT STRAND 134..139
FT /evidence="ECO:0007829|PDB:6BLB"
FT STRAND 148..152
FT /evidence="ECO:0007829|PDB:6BLB"
FT STRAND 157..163
FT /evidence="ECO:0007829|PDB:6BLB"
FT HELIX 165..167
FT /evidence="ECO:0007829|PDB:6BLB"
FT HELIX 170..174
FT /evidence="ECO:0007829|PDB:6BLB"
FT STRAND 177..181
FT /evidence="ECO:0007829|PDB:6BLB"
FT HELIX 187..200
FT /evidence="ECO:0007829|PDB:6BLB"
FT HELIX 207..215
FT /evidence="ECO:0007829|PDB:6BLB"
FT HELIX 221..238
FT /evidence="ECO:0007829|PDB:6BLB"
FT STRAND 239..243
FT /evidence="ECO:0007829|PDB:6BLB"
FT HELIX 245..254
FT /evidence="ECO:0007829|PDB:6BLB"
FT HELIX 264..277
FT /evidence="ECO:0007829|PDB:6BLB"
FT HELIX 284..291
FT /evidence="ECO:0007829|PDB:6BLB"
FT HELIX 295..300
FT /evidence="ECO:0007829|PDB:6BLB"
FT HELIX 303..308
FT /evidence="ECO:0007829|PDB:6BLB"
FT STRAND 311..315
FT /evidence="ECO:0007829|PDB:6BLB"
FT STRAND 318..321
FT /evidence="ECO:0007829|PDB:6BLB"
FT HELIX 323..328
FT /evidence="ECO:0007829|PDB:6BLB"
SQ SEQUENCE 352 AA; 38933 MW; F24EE2804F12D7F1 CRC64;
MIEPDRLISA VSGRERDEQL DRAIRPLKLA DYIGQPSVRE QMELFIHAAR GRQEALDHTL
IFGPPGLGKT TLANIIAQEM GVSIKSTSGP VLERPGDLAA LLTNLEAGDV LFVDEIHRLS
PIVEEVLYPA MEDFQLDIMI GEGPAARSIK LDLPPFTLVG ATTRAGMLTN PLRDRFGIVQ
RLEFYNVEDL ATIVSRSAGI LGLEIEPQGA AEIAKRARGT PRIANRLLRR VRDFAEVRGQ
GDITRVIADK ALNLLDVDER GFDHLDRRLL LTMIDKFDGG PVGIDNLAAA LSEERHTIED
VLEPYLIQQG YIMRTPRGRV VTRHAYLHFG LNIPKRLGPG VTTDLFTSED GN
