ID   RUVB_STRPN              Reviewed;         332 AA.
AC   Q97SR6;
DT   03-APR-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   03-AUG-2022, entry version 121.
DE   RecName: Full=Holliday junction ATP-dependent DNA helicase RuvB {ECO:0000255|HAMAP-Rule:MF_00016};
DE            EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_00016};
GN   Name=ruvB {ECO:0000255|HAMAP-Rule:MF_00016}; OrderedLocusNames=SP_0259;
OS   Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=170187;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-334 / TIGR4;
RX   PubMed=11463916; DOI=10.1126/science.1061217;
RA   Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D.,
RA   Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J.,
RA   Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D.,
RA   Umayam L.A., White O., Salzberg S.L., Lewis M.R., Radune D.,
RA   Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L.,
RA   McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K.,
RA   Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A.,
RA   Morrison D.A., Hollingshead S.K., Fraser C.M.;
RT   "Complete genome sequence of a virulent isolate of Streptococcus
RT   pneumoniae.";
RL   Science 293:498-506(2001).
CC   -!- FUNCTION: The RuvA-RuvB complex in the presence of ATP renatures
CC       cruciform structure in supercoiled DNA with palindromic sequence,
CC       indicating that it may promote strand exchange reactions in homologous
CC       recombination. RuvAB is a helicase that mediates the Holliday junction
CC       migration by localized denaturation and reannealing.
CC       {ECO:0000255|HAMAP-Rule:MF_00016}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00016};
CC   -!- SUBUNIT: Forms a complex with RuvA. {ECO:0000255|HAMAP-Rule:MF_00016}.
CC   -!- INTERACTION:
CC       Q97SR6; Q38002: orf16; Xeno; NbExp=2; IntAct=EBI-11616013, EBI-3990347;
CC       Q97SR6; E7DN73; Xeno; NbExp=2; IntAct=EBI-11616013, EBI-11703857;
CC       Q97SR6; E7DN80; Xeno; NbExp=2; IntAct=EBI-11616013, EBI-3989706;
CC       Q97SR6; E7DN95; Xeno; NbExp=2; IntAct=EBI-11616013, EBI-11704441;
CC       Q97SR6; E7DN96; Xeno; NbExp=2; IntAct=EBI-11616013, EBI-11704462;
CC       Q97SR6; E7DNA3; Xeno; NbExp=2; IntAct=EBI-11616013, EBI-11704561;
CC   -!- SIMILARITY: Belongs to the RuvB family. {ECO:0000255|HAMAP-
CC       Rule:MF_00016}.
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DR   EMBL; AE005672; AAK74437.1; -; Genomic_DNA.
DR   PIR; D95030; D95030.
DR   RefSeq; WP_001808442.1; NZ_AKVY01000001.1.
DR   AlphaFoldDB; Q97SR6; -.
DR   SMR; Q97SR6; -.
DR   IntAct; Q97SR6; 6.
DR   STRING; 170187.SP_0259; -.
DR   EnsemblBacteria; AAK74437; AAK74437; SP_0259.
DR   KEGG; spn:SP_0259; -.
DR   eggNOG; COG2255; Bacteria.
DR   OMA; IHRMSRP; -.
DR   PhylomeDB; Q97SR6; -.
DR   BioCyc; SPNE170187:G1FZB-266-MON; -.
DR   Proteomes; UP000000585; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0009378; F:four-way junction helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0009432; P:SOS response; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00016; DNA_helic_RuvB; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR041445; AAA_lid_4.
DR   InterPro; IPR004605; DNA_helicase_Holl-junc_RuvB.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR008824; RuvB-like_N.
DR   InterPro; IPR008823; RuvB_C.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR42848; PTHR42848; 1.
DR   Pfam; PF17864; AAA_lid_4; 1.
DR   Pfam; PF05491; RuvB_C; 1.
DR   Pfam; PF05496; RuvB_N; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00635; ruvB; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; DNA damage; DNA recombination; DNA repair; Helicase;
KW   Hydrolase; Nucleotide-binding; SOS response.
FT   CHAIN           1..332
FT                   /note="Holliday junction ATP-dependent DNA helicase RuvB"
FT                   /id="PRO_0000165607"
FT   BINDING         59..66
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00016"
SQ   SEQUENCE   332 AA;  37279 MW;  5D61C45820B6E421 CRC64;
     MSRILDNEMM GDEELVERTL RPQYLREYIG QDKVKDQLQI FIEAAKMRDE ALDHVLLFGP
     PGLGKTTMAF VIANELGVNL KQTSGPVIEK AGDLVAILND LEPGDVLFID EIHRLPMSVE
     EVLYSAMEDF YIDIMIGAGE GSRSVHLELP PFTLIGATTR AGMLSNPLRA RFGITGHMEY
     YAHADLTEIV ERTADIFEME ITHEAASELA LRSRGTPRIA NRLLKRVRDF AQIMGNGVID
     DVITDKALTM LDVDHEGLDY VDQKILRTMI EMYSGGPVGL GTLSVNIAEE RETVEDMYEP
     YLIQKGFIMR TRSGRVATAK AYEHLGYEYS EK