ID   RUVB_SYNY3              Reviewed;         361 AA.
AC   Q57396;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 139.
DE   RecName: Full=Holliday junction ATP-dependent DNA helicase RuvB {ECO:0000255|HAMAP-Rule:MF_00016};
DE            EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_00016};
GN   Name=ruvB {ECO:0000255|HAMAP-Rule:MF_00016}; OrderedLocusNames=sll0613;
OS   Synechocystis sp. (strain PCC 6803 / Kazusa).
OC   Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC   unclassified Synechocystis.
OX   NCBI_TaxID=1111708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=PCC 6803 / Kazusa;
RA   Bulteau S., Cassier-Chauvat C., Chauvat F.;
RT   "Cloning, expression and inactivation of the ruvB gene from the
RT   cyanobacterium Synechocystis PCC6803.";
RL   Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27184 / PCC 6803 / N-1;
RX   PubMed=8590279; DOI=10.1093/dnares/2.4.153;
RA   Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N.,
RA   Sugiura M., Tabata S.;
RT   "Sequence analysis of the genome of the unicellular cyanobacterium
RT   Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region
RT   from map positions 64% to 92% of the genome.";
RL   DNA Res. 2:153-166(1995).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA   Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA   Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA   Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA   Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence analysis of the genome of the unicellular cyanobacterium
RT   Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT   genome and assignment of potential protein-coding regions.";
RL   DNA Res. 3:109-136(1996).
RN   [4]
RP   FUNCTION, INDUCTION BY UV, AND DISRUPTION PHENOTYPE.
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=15225304; DOI=10.1111/j.1365-2958.2004.04100.x;
RA   Domain F., Houot L., Chauvat F., Cassier-Chauvat C.;
RT   "Function and regulation of the cyanobacterial genes lexA, recA and ruvB:
RT   LexA is critical to the survival of cells facing inorganic carbon
RT   starvation.";
RL   Mol. Microbiol. 53:65-80(2004).
CC   -!- FUNCTION: The RuvA-RuvB complex in the presence of ATP renatures
CC       cruciform structure in supercoiled DNA with palindromic sequence,
CC       indicating that it may promote strand exchange reactions in homologous
CC       recombination. RuvAB is a helicase that mediates the Holliday junction
CC       migration by localized denaturation and reannealing.
CC       {ECO:0000255|HAMAP-Rule:MF_00016}.
CC   -!- FUNCTION: Participates in UV-tolerance of Synechocystis PCC 6803.
CC       {ECO:0000269|PubMed:15225304}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00016};
CC   -!- SUBUNIT: Forms a complex with RuvA. {ECO:0000255|HAMAP-Rule:MF_00016}.
CC   -!- INDUCTION: 2-3 fold induced by UV light. {ECO:0000305|PubMed:15225304}.
CC   -!- DISRUPTION PHENOTYPE: No visible phenotype; cells are more sensitive to
CC       UV-C light, H(2)O(2), CdSO(4), SeO(3) and SeO(4).
CC       {ECO:0000269|PubMed:15225304}.
CC   -!- MISCELLANEOUS: This bacterium is considerably more resistant to UV and
CC       gamma irradiation than E.coli; the E.coli-like SOS regulon model is not
CC       an appropriate model for DNA repair in this cyanobacterium.
CC       {ECO:0000305|PubMed:15225304}.
CC   -!- SIMILARITY: Belongs to the RuvB family. {ECO:0000255|HAMAP-
CC       Rule:MF_00016}.
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DR   EMBL; U38892; AAA96396.1; -; Genomic_DNA.
DR   EMBL; BA000022; BAA10350.1; -; Genomic_DNA.
DR   PIR; S76504; S76504.
DR   AlphaFoldDB; Q57396; -.
DR   SMR; Q57396; -.
DR   STRING; 1148.1001619; -.
DR   PaxDb; Q57396; -.
DR   EnsemblBacteria; BAA10350; BAA10350; BAA10350.
DR   KEGG; syn:sll0613; -.
DR   eggNOG; COG2255; Bacteria.
DR   InParanoid; Q57396; -.
DR   OMA; IHRMSRP; -.
DR   PhylomeDB; Q57396; -.
DR   Proteomes; UP000001425; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0009378; F:four-way junction helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0009432; P:SOS response; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00016; DNA_helic_RuvB; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR041445; AAA_lid_4.
DR   InterPro; IPR004605; DNA_helicase_Holl-junc_RuvB.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR008824; RuvB-like_N.
DR   InterPro; IPR008823; RuvB_C.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR42848; PTHR42848; 1.
DR   Pfam; PF17864; AAA_lid_4; 1.
DR   Pfam; PF05491; RuvB_C; 1.
DR   Pfam; PF05496; RuvB_N; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00635; ruvB; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; DNA recombination; Helicase; Hydrolase; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN           1..361
FT                   /note="Holliday junction ATP-dependent DNA helicase RuvB"
FT                   /id="PRO_0000165616"
FT   REGION          1..41
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..17
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        24..39
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         81..88
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00016"
SQ   SEQUENCE   361 AA;  40053 MW;  062E0D70AA3710D3 CRC64;
     MAIKRSGNNN LSPNVKSDLL SPEVIPQERS TSPELEQQEA SLRPQRLADY IGQRDLKEVL
     RIAIQAAQGR QEAIDHLLLY GPPGLGKTTL ALILAEEMQV RCKITAAPAL ERPRDITGLL
     LALQPGDILF IDEIHRLNRL TEELLYPAME DFRLDITMGK GQSAKVRSLK LAHFTLVGAT
     TKVGSLTSPL RDRFGLIQRL RFYEVDELQQ IILRTAGILS VSISPTGAEA IAMRARGTPR
     IANRLLKRVR DYAQVKQQPE IDPALASEAL DLYQVDKRGL DWTDRLVLQT LIEQFQGGPT
     GLEAIAAATG EDAKTIEEVY EPYLLQIGYL ARTSRGRIAT TAAYEHLGLT PPTPLLPWKE
     S