BDS3A_BUNGR
ID BDS3A_BUNGR Reviewed; 74 AA.
AC G0W2H8;
DT 22-JUL-2015, integrated into UniProtKB/Swiss-Prot.
DT 19-OCT-2011, sequence version 1.
DT 25-MAY-2022, entry version 21.
DE RecName: Full=U-actitoxin-Bgr3a {ECO:0000303|PubMed:22683676};
DE Short=U-AITX-Bgr3a {ECO:0000303|PubMed:22683676};
DE AltName: Full=U-AITX-Bg1b {ECO:0000303|PubMed:22015268};
DE Flags: Precursor;
OS Bunodosoma granuliferum (Red warty sea anemone).
OC Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC Actiniidae; Bunodosoma.
OX NCBI_TaxID=31164;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND MASS SPECTROMETRY.
RX PubMed=22015268; DOI=10.1016/j.peptides.2011.10.011;
RA Rodriguez A.A., Cassoli J.S., Sa F., Dong Z.Q., de Freitas J.C.,
RA Pimenta A.M., de Lima M.E., Konno K., Lee S.M., Garateix A.,
RA Zaharenko A.J.;
RT "Peptide fingerprinting of the neurotoxic fractions isolated from the
RT secretions of sea anemones Stichodactyla helianthus and Bunodosoma
RT granulifera. New members of the APETx-like family identified by a 454
RT pyrosequencing approach.";
RL Peptides 34:26-38(2012).
RN [2]
RP NOMENCLATURE.
RX PubMed=22683676; DOI=10.1016/j.toxicon.2012.05.020;
RA Oliveira J.S., Fuentes-Silva D., King G.F.;
RT "Development of a rational nomenclature for naming peptide and protein
RT toxins from sea anemones.";
RL Toxicon 60:539-550(2012).
CC -!- FUNCTION: Potently and selectively inhibits voltage-gated potassium
CC channels Kv11/KCNH/ERG. Acts as a gating-modifier toxin that shifts the
CC voltage-dependence of ERG activation in the positive direction and
CC suppresses its current amplitudes elicited by strong depolarizing
CC pulses that maximally activate the channels.
CC {ECO:0000250|UniProtKB:P61541}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}. Nematocyst {ECO:0000305}.
CC -!- MASS SPECTROMETRY: Mass=4589.0; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:22015268};
CC -!- MISCELLANEOUS: Does not show effect on crabs.
CC {ECO:0000269|PubMed:22015268}.
CC -!- SIMILARITY: Belongs to the sea anemone type 3 (BDS) potassium channel
CC toxin family. {ECO:0000305}.
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DR EMBL; HE577145; CCC86603.1; -; mRNA.
DR AlphaFoldDB; G0W2H8; -.
DR SMR; G0W2H8; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0042151; C:nematocyst; IEA:UniProtKB-SubCell.
DR GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR Gene3D; 2.20.20.10; -; 1.
DR InterPro; IPR012414; BDS_K_chnl_tox.
DR InterPro; IPR023355; Myo_ane_neurotoxin_sf.
DR Pfam; PF07936; Defensin_4; 1.
PE 1: Evidence at protein level;
KW Cleavage on pair of basic residues; Disulfide bond;
KW Ion channel impairing toxin; Nematocyst; Neurotoxin;
KW Potassium channel impairing toxin; Secreted; Signal; Toxin;
KW Voltage-gated potassium channel impairing toxin.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT PROPEP 22..29
FT /evidence="ECO:0000269|PubMed:22015268"
FT /id="PRO_0000433587"
FT CHAIN 32..74
FT /note="U-actitoxin-Bgr3a"
FT /evidence="ECO:0000269|PubMed:22015268"
FT /id="PRO_0000433588"
FT DISULFID 35..68
FT /evidence="ECO:0000250|UniProtKB:P61541"
FT DISULFID 37..61
FT /evidence="ECO:0000250|UniProtKB:P61541"
FT DISULFID 51..69
FT /evidence="ECO:0000250|UniProtKB:P61541"
SQ SEQUENCE 74 AA; 7963 MW; 693E3542BE4C6647 CRC64;
MSAQRFLFLL VVTSLIAASL AAPKDVQLTK RGTPCWCGKT VGIYWFALYS CPGGHGYTGH
CGQFMGVCCY PADP