ID   RUVC_MYCTU              Reviewed;         188 AA.
AC   P9WGV9; L0TA48; P66760; Q50627;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 39.
DE   RecName: Full=Crossover junction endodeoxyribonuclease RuvC;
DE            EC=3.1.21.10;
DE   AltName: Full=Holliday junction nuclease RuvC;
DE   AltName: Full=Holliday junction resolvase RuvC;
GN   Name=ruvC; OrderedLocusNames=Rv2594c; ORFNames=MTCY227.07;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
CC   -!- FUNCTION: Nuclease that resolves Holliday junction intermediates in
CC       genetic recombination. Cleaves the cruciform structure in supercoiled
CC       DNA by nicking to strands with the same polarity at sites symmetrically
CC       opposed at the junction in the homologous arms and leaves a 5'-terminal
CC       phosphate and a 3'-terminal hydroxyl group (By similarity).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage at a junction such as a reciprocal
CC         single-stranded crossover between two homologous DNA duplexes
CC         (Holliday junction).; EC=3.1.21.10;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the RuvC family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP45390.1; -; Genomic_DNA.
DR   PIR; A70727; A70727.
DR   RefSeq; NP_217110.1; NC_000962.3.
DR   RefSeq; WP_003413426.1; NZ_NVQJ01000023.1.
DR   AlphaFoldDB; P9WGV9; -.
DR   SMR; P9WGV9; -.
DR   STRING; 83332.Rv2594c; -.
DR   PaxDb; P9WGV9; -.
DR   DNASU; 887418; -.
DR   GeneID; 45426596; -.
DR   GeneID; 887418; -.
DR   KEGG; mtu:Rv2594c; -.
DR   TubercuList; Rv2594c; -.
DR   eggNOG; COG0817; Bacteria.
DR   OMA; AICHIWR; -.
DR   PhylomeDB; P9WGV9; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0008821; F:crossover junction endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006974; P:cellular response to DNA damage stimulus; IEP:MTBBASE.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   CDD; cd16962; RuvC; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   HAMAP; MF_00034; RuvC; 1.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   InterPro; IPR020563; X-over_junc_endoDNase_Mg_BS.
DR   InterPro; IPR002176; X-over_junc_endoDNase_RuvC.
DR   PANTHER; PTHR30194; PTHR30194; 1.
DR   Pfam; PF02075; RuvC; 1.
DR   PRINTS; PR00696; RSOLVASERUVC.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   TIGRFAMs; TIGR00228; ruvC; 1.
DR   PROSITE; PS01321; RUVC; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA recombination; DNA repair; Hydrolase; Magnesium;
KW   Metal-binding; Nuclease; Reference proteome.
FT   CHAIN           1..188
FT                   /note="Crossover junction endodeoxyribonuclease RuvC"
FT                   /id="PRO_0000183110"
FT   BINDING         7
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         68
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         141
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         144
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   188 AA;  19786 MW;  1D8619C9150406FB CRC64;
     MRVMGVDPGL TRCGLSLIES GRGRQLTALD VDVVRTPSDA ALAQRLLAIS DAVEHWLDTH
     HPEVVAIERV FSQLNVTTVM GTAQAGGVIA LAAAKRGVDV HFHTPSEVKA AVTGNGSADK
     AQVTAMVTKI LALQAKPTPA DAADALALAI CHCWRAPTIA RMAEATSRAE ARAAQQRHAY
     LAKLKAAR