ID   BEA4_GIBF5              Reviewed;         633 AA.
AC   S0EFU6;
DT   25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT   18-SEP-2013, sequence version 1.
DT   25-MAY-2022, entry version 28.
DE   RecName: Full=Beauvericin cluster-specific repressor BEA4 {ECO:0000303|PubMed:27750383};
DE   AltName: Full=Beauvericin biosynthesis cluster protein 4 {ECO:0000303|PubMed:27750383};
GN   Name=BEA4 {ECO:0000303|PubMed:27750383}; ORFNames=FFUJ_09298;
OS   Gibberella fujikuroi (strain CBS 195.34 / IMI 58289 / NRRL A-6831) (Bakanae
OS   and foot rot disease fungus) (Fusarium fujikuroi).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium;
OC   Fusarium fujikuroi species complex.
OX   NCBI_TaxID=1279085;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CBS 195.34 / IMI 58289 / NRRL A-6831;
RX   PubMed=23825955; DOI=10.1371/journal.ppat.1003475;
RA   Wiemann P., Sieber C.M.K., von Bargen K.W., Studt L., Niehaus E.-M.,
RA   Espino J.J., Huss K., Michielse C.B., Albermann S., Wagner D.,
RA   Bergner S.V., Connolly L.R., Fischer A., Reuter G., Kleigrewe K., Bald T.,
RA   Wingfield B.D., Ophir R., Freeman S., Hippler M., Smith K.M., Brown D.W.,
RA   Proctor R.H., Muensterkoetter M., Freitag M., Humpf H.-U., Gueldener U.,
RA   Tudzynski B.;
RT   "Deciphering the cryptic genome: genome-wide analyses of the rice pathogen
RT   Fusarium fujikuroi reveal complex regulation of secondary metabolism and
RT   novel metabolites.";
RL   PLoS Pathog. 9:E1003475-E1003475(2013).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=27750383; DOI=10.1111/1462-2920.13576;
RA   Niehaus E.M., Studt L., von Bargen K.W., Kummer W., Humpf H.U., Reuter G.,
RA   Tudzynski B.;
RT   "Sound of silence: the beauvericin cluster in Fusarium fujikuroi is
RT   controlled by cluster-specific and global regulators mediated by H3K27
RT   modification.";
RL   Environ. Microbiol. 18:4282-4302(2016).
CC   -!- FUNCTION: Transcription factor that specifically represses the
CC       expression of the gene cluster involved in the biosynthesis of
CC       beauvericin (BEA), a non-ribosomal cyclic hexadepsipeptide that shows
CC       antibiotic, antifungal, insecticidal, and cancer cell antiproliferative
CC       and antihaptotactic activity (PubMed:27750383).
CC       {ECO:0000269|PubMed:27750383}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC   -!- DISRUPTION PHENOTYPE: Leads to elevated expression of BEA1, BEA2 and
CC       BEA3 (PubMed:27750383). {ECO:0000269|PubMed:27750383}.
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DR   EMBL; HF679031; CCT73876.1; -; Genomic_DNA.
DR   AlphaFoldDB; S0EFU6; -.
DR   SMR; S0EFU6; -.
DR   EnsemblFungi; CCT73876; CCT73876; FFUJ_09298.
DR   VEuPathDB; FungiDB:FFUJ_09298; -.
DR   HOGENOM; CLU_014597_0_0_1; -.
DR   Proteomes; UP000016800; Chromosome 9.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR021858; Fun_TF.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   Pfam; PF11951; Fungal_trans_2; 1.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation; Zinc.
FT   CHAIN           1..633
FT                   /note="Beauvericin cluster-specific repressor BEA4"
FT                   /id="PRO_0000442149"
FT   DNA_BIND        19..46
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT   REGION          87..143
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        87..121
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   633 AA;  70600 MW;  042D79937643FB6F CRC64;
     MTDESKRCRT KKVKARTGCS TCKRRKVKCD EQLPGCKRCA KIGVECPGYH KPVKWSVKYE
     RHLVNSSPTA GNSFSCFDSG AGNLSNLMQP KSNPGSVETQ NHNDNEVQAP GPLTRGSSSS
     LSDAREHLED TLEMQPPSTL NSEQLSTLCD DVQADEEFPC ALNALHDDSL CLTSYIPTLQ
     TSLILDHYFT TVCHFNSSFD SANNPFRSEV SRMMADSPLL FGCVLSMSAA HLYQGDKSSS
     CIPLEFQTEA MSHLSRTLSE LPTRNECEVD DNRPMALVSR EKILNVSDDL LLSIIFLGMT
     AAWHDVSATG LPHLHGSRQL FRSWISLNNL TDIEKRRKMT RTQIFVVSSM VYWEAMSSAL
     FDQQYEGLSH LTMFCDPHPP ALVQPCPWTG VATPIFVFLA KTLTLVRNNQ ALKSLWVFES
     GEIHRRALYG ELLAKANSLE REILRYRLPS LALIEDIGDP CTTPDHLLAI SRCYRMAALL
     ELYSAFPEIT RTGGRPDSAI DLDRGDEKTH LIMGLAFSIL EILEKIPDDS GTISIQLLSL
     LIAGSVLGPV PPRGEDEGPK RLETLRLRTF VRQRIHKMYA AVRLGPIGNA MAILEEVWAR
     MDMLPAIQNA NSAISSFHWI NIMSQKKLET MFG