ID   BEBT_CLABR              Reviewed;         456 AA.
AC   Q8GT21;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 52.
DE   RecName: Full=Benzyl alcohol O-benzoyltransferase;
DE            EC=2.3.1.196;
DE   AltName: Full=Benzoyl coenzyme A:benzyl alcohol benzoyl transferase;
OS   Clarkia breweri (Fairy fans) (Eucharidium breweri).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Myrtales; Onagraceae; Onagroideae; Onagreae; Clarkia.
OX   NCBI_TaxID=36903;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL
RP   PROPERTIES, ACTIVITY REGULATION, SUBUNIT, TISSUE SPECIFICITY, DEVELOPMENTAL
RP   STAGE, INDUCTION BY WOUNDING, AND FUNCTION.
RX   PubMed=12226525; DOI=10.1104/pp.006460;
RA   D'Auria J.C., Chen F., Pichersky E.;
RT   "Characterization of an acyltransferase capable of synthesizing
RT   benzylbenzoate and other volatile esters in flowers and damaged leaves of
RT   Clarkia breweri.";
RL   Plant Physiol. 130:466-476(2002).
CC   -!- FUNCTION: Probably involved in the formation of benzylbenzoate, a minor
CC       constituent of the floral aroma. {ECO:0000269|PubMed:12226525}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=benzoyl-CoA + benzyl alcohol = benzyl benzoate + CoA;
CC         Xref=Rhea:RHEA:30411, ChEBI:CHEBI:17987, ChEBI:CHEBI:41237,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57369; EC=2.3.1.196;
CC         Evidence={ECO:0000269|PubMed:12226525};
CC   -!- ACTIVITY REGULATION: Inhibited by magnesium, calcium, cobalt, zinc and
CC       copper. {ECO:0000269|PubMed:12226525}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=46.8 uM for benzyl alcohol (with benzoyl-CoA as cosubstrate)
CC         {ECO:0000269|PubMed:12226525};
CC         KM=97.8 uM for cinnamyl alcohol (with benzoyl-CoA as cosubstrate)
CC         {ECO:0000269|PubMed:12226525};
CC         KM=20.5 uM for benzoyl-CoA (with benzyl alcohol as cosubstrate)
CC         {ECO:0000269|PubMed:12226525};
CC         KM=464 uM for cinnamoyl-CoA (with benzyl alcohol as cosubstrate)
CC         {ECO:0000269|PubMed:12226525};
CC         KM=818 uM for acetyl-CoA (with benzyl alcohol as cosubstrate)
CC         {ECO:0000269|PubMed:12226525};
CC       pH dependence:
CC         Optimum pH is 7.7. {ECO:0000269|PubMed:12226525};
CC   -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:12226525}.
CC   -!- TISSUE SPECIFICITY: Highly expressed in stigma. Detected in stamens,
CC       sepals, petals and style. Bearly expressed in leaves.
CC       {ECO:0000269|PubMed:12226525}.
CC   -!- DEVELOPMENTAL STAGE: Highly expressed in the stigma of unopened
CC       flowers, peaks on day 1 postanthesis and drops rapidly on day 2
CC       postanthesis when the stigma becomes receptive (at protein level).
CC       {ECO:0000269|PubMed:12226525}.
CC   -!- INDUCTION: Up-regulated by wounding. Expression peaks 6 hours after
CC       wonding, decreasing afterward. {ECO:0000269|PubMed:12226525}.
CC   -!- SIMILARITY: Belongs to the plant acyltransferase family. {ECO:0000305}.
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DR   EMBL; AF500200; AAN09796.1; -; mRNA.
DR   AlphaFoldDB; Q8GT21; -.
DR   SMR; Q8GT21; -.
DR   KEGG; ag:AAN09796; -.
DR   BRENDA; 2.3.1.196; 1437.
DR   SABIO-RK; Q8GT21; -.
DR   GO; GO:0016746; F:acyltransferase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.559.10; -; 2.
DR   InterPro; IPR023213; CAT-like_dom_sf.
PE   1: Evidence at protein level;
KW   Acyltransferase; Transferase.
FT   CHAIN           1..456
FT                   /note="Benzyl alcohol O-benzoyltransferase"
FT                   /id="PRO_0000409590"
FT   ACT_SITE        166
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        380
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   456 AA;  50650 MW;  E335098512DCE27A CRC64;
     MAHDQSLSFE VCRRKPELIR PAKQTPHEFK KLSDVEDQEG LRFQIPVIQF YKHNNESMQE
     RDPVQVIREG IARALVYYYP FAGRLREVDG RKLVVECTGE GVMFIEADAD VTLEQFGDAL
     QPPFPCFDQL LFDVPGSGGI LDSPLLLIQV TRLKCGSFIF ALRLNHTMAD AAGIVLFMKA
     VGEMARGAAT PSTLPVWDRH ILNARVPPQV TFNHREYEEV KGTIFTPFDD LAHRSFFFGS
     TEISAMRKQI PPHLRSCSTT IEVLTACLWR CRTLAIKPNP DEEVRMICIV NARSKFNPPL
     PDGYYGNAFA IPAAVTTAGK LCNNPLGFAL ELIRKAKREV TEEYMHSVAD LMVATGRPHF
     TVVNTYLVSD VTRAGFGEVD FGWGEAVYGG PAKGGVGVIP GVTSFYIPLR NRQGEKGIVL
     PICLPSAAME IFAEALNNTL NGKEIEIAKH FTQSSL