RUVC_THET8
ID RUVC_THET8 Reviewed; 166 AA.
AC Q5SJC4;
DT 07-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Crossover junction endodeoxyribonuclease RuvC {ECO:0000255|HAMAP-Rule:MF_00034};
DE EC=3.1.21.10 {ECO:0000255|HAMAP-Rule:MF_00034};
DE AltName: Full=Holliday junction nuclease RuvC {ECO:0000255|HAMAP-Rule:MF_00034};
DE AltName: Full=Holliday junction resolvase RuvC {ECO:0000255|HAMAP-Rule:MF_00034};
GN Name=ruvC {ECO:0000255|HAMAP-Rule:MF_00034}; OrderedLocusNames=TTHA1090;
OS Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).
OC Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX NCBI_TaxID=300852;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27634 / DSM 579 / HB8;
RA Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T.,
RA Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.;
RT "Complete genome sequence of Thermus thermophilus HB8.";
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Nuclease that resolves Holliday junction intermediates in
CC genetic recombination. Cleaves the cruciform structure in supercoiled
CC DNA by nicking to strands with the same polarity at sites symmetrically
CC opposed at the junction in the homologous arms and leaves a 5'-terminal
CC phosphate and a 3'-terminal hydroxyl group. {ECO:0000255|HAMAP-
CC Rule:MF_00034}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage at a junction such as a reciprocal
CC single-stranded crossover between two homologous DNA duplexes
CC (Holliday junction).; EC=3.1.21.10; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00034};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00034};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_00034};
CC -!- SIMILARITY: Belongs to the RuvC family. {ECO:0000255|HAMAP-
CC Rule:MF_00034}.
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DR EMBL; AP008226; BAD70913.1; -; Genomic_DNA.
DR RefSeq; WP_011173164.1; NC_006461.1.
DR RefSeq; YP_144356.1; NC_006461.1.
DR PDB; 4EP4; X-ray; 1.28 A; A/B=1-166.
DR PDB; 4EP5; X-ray; 2.08 A; A=1-166.
DR PDB; 4LD0; X-ray; 3.75 A; A/B=1-166.
DR PDB; 6S16; X-ray; 3.41 A; A/B=1-166.
DR PDBsum; 4EP4; -.
DR PDBsum; 4EP5; -.
DR PDBsum; 4LD0; -.
DR PDBsum; 6S16; -.
DR AlphaFoldDB; Q5SJC4; -.
DR SMR; Q5SJC4; -.
DR STRING; 300852.55772472; -.
DR EnsemblBacteria; BAD70913; BAD70913; BAD70913.
DR GeneID; 3168165; -.
DR KEGG; ttj:TTHA1090; -.
DR PATRIC; fig|300852.9.peg.1070; -.
DR eggNOG; COG0817; Bacteria.
DR HOGENOM; CLU_091257_3_1_0; -.
DR OMA; AICHIWR; -.
DR PhylomeDB; Q5SJC4; -.
DR BRENDA; 3.1.21.10; 2305.
DR Proteomes; UP000000532; Chromosome.
DR GO; GO:0008821; F:crossover junction endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR CDD; cd16962; RuvC; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR HAMAP; MF_00034; RuvC; 1.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR InterPro; IPR002176; X-over_junc_endoDNase_RuvC.
DR PANTHER; PTHR30194; PTHR30194; 1.
DR Pfam; PF02075; RuvC; 1.
DR PRINTS; PR00696; RSOLVASERUVC.
DR SUPFAM; SSF53098; SSF53098; 1.
DR TIGRFAMs; TIGR00228; ruvC; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA damage; DNA recombination; DNA repair; Hydrolase;
KW Magnesium; Metal-binding; Nuclease; Reference proteome.
FT CHAIN 1..166
FT /note="Crossover junction endodeoxyribonuclease RuvC"
FT /id="PRO_0000225184"
FT BINDING 7
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00034"
FT BINDING 70
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00034"
FT BINDING 143
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00034"
FT BINDING 146
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00034"
FT STRAND 2..7
FT /evidence="ECO:0007829|PDB:4EP4"
FT STRAND 10..20
FT /evidence="ECO:0007829|PDB:4EP4"
FT STRAND 22..26
FT /evidence="ECO:0007829|PDB:4EP4"
FT STRAND 28..37
FT /evidence="ECO:0007829|PDB:4EP4"
FT STRAND 40..42
FT /evidence="ECO:0007829|PDB:4EP5"
FT HELIX 44..62
FT /evidence="ECO:0007829|PDB:4EP4"
FT STRAND 65..70
FT /evidence="ECO:0007829|PDB:4EP4"
FT HELIX 79..98
FT /evidence="ECO:0007829|PDB:4EP4"
FT STRAND 102..105
FT /evidence="ECO:0007829|PDB:4EP4"
FT HELIX 107..115
FT /evidence="ECO:0007829|PDB:4EP4"
FT STRAND 117..119
FT /evidence="ECO:0007829|PDB:6S16"
FT HELIX 122..132
FT /evidence="ECO:0007829|PDB:4EP4"
FT HELIX 142..159
FT /evidence="ECO:0007829|PDB:4EP4"
SQ SEQUENCE 166 AA; 17755 MW; 214FBE6E1D4A91C3 CRC64;
MVVAGIDPGI THLGLGVVAV EGKGALKARL LHGEVVKTSP QEPAKERVGR IHARVLEVLH
RFRPEAVAVE EQFFYRQNEL AYKVGWALGA VLVAAFEAGV PVYAYGPMQV KQALAGHGHA
AKEEVALMVR GILGLKEAPR PSHLADALAI ALTHAFYARM GTAKPL
