ID   BEBT_TOBAC              Reviewed;         460 AA.
AC   Q8GT20;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 57.
DE   RecName: Full=Benzyl alcohol O-benzoyltransferase;
DE            EC=2.3.1.196;
DE   AltName: Full=Benzoyl coenzyme A:benzyl alcohol benzoyl transferase;
GN   Name=HSR201;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL
RP   PROPERTIES, AND FUNCTION.
RX   PubMed=12226525; DOI=10.1104/pp.006460;
RA   D'Auria J.C., Chen F., Pichersky E.;
RT   "Characterization of an acyltransferase capable of synthesizing
RT   benzylbenzoate and other volatile esters in flowers and damaged leaves of
RT   Clarkia breweri.";
RL   Plant Physiol. 130:466-476(2002).
RN   [2]
RP   INDUCTION BY PATHOGEN INFECTION.
RX   PubMed=8756591; DOI=10.1007/bf00021788;
RA   Czernic P., Huang H.C., Marco Y.;
RT   "Characterization of hsr201 and hsr515, two tobacco genes preferentially
RT   expressed during the hypersensitive reaction provoked by phytopathogenic
RT   bacteria.";
RL   Plant Mol. Biol. 31:255-265(1996).
CC   -!- FUNCTION: Probably involved in the formation of volatile ester
CC       benzylbenzoate. {ECO:0000269|PubMed:12226525}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=benzoyl-CoA + benzyl alcohol = benzyl benzoate + CoA;
CC         Xref=Rhea:RHEA:30411, ChEBI:CHEBI:17987, ChEBI:CHEBI:41237,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57369; EC=2.3.1.196;
CC         Evidence={ECO:0000269|PubMed:12226525};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=19 uM for benzyl alcohol (with benzoyl-CoA as cosubstrate)
CC         {ECO:0000269|PubMed:12226525};
CC         KM=35 uM for benzoyl-CoA (with benzyl alcohol as cosubstrate)
CC         {ECO:0000269|PubMed:12226525};
CC   -!- INDUCTION: Up-regulated in the leaf during the hypersensitive reaction
CC       provoked by a pathogen infection. {ECO:0000269|PubMed:8756591}.
CC   -!- SIMILARITY: Belongs to the plant acyltransferase family. {ECO:0000305}.
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DR   EMBL; AF500202; AAN09798.1; -; mRNA.
DR   RefSeq; NP_001312867.1; NM_001325938.1.
DR   AlphaFoldDB; Q8GT20; -.
DR   SMR; Q8GT20; -.
DR   PRIDE; Q8GT20; -.
DR   GeneID; 107814928; -.
DR   KEGG; nta:107814928; -.
DR   BRENDA; 2.3.1.196; 3645.
DR   SABIO-RK; Q8GT20; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IBA:GO_Central.
DR   Gene3D; 3.30.559.10; -; 2.
DR   InterPro; IPR023213; CAT-like_dom_sf.
PE   1: Evidence at protein level;
KW   Acyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..460
FT                   /note="Benzyl alcohol O-benzoyltransferase"
FT                   /id="PRO_0000409591"
FT   ACT_SITE        167
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        382
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   460 AA;  51014 MW;  4A2A2CA63394EF20 CRC64;
     MDSKQSSELV FTVRRQKPEL IAPAKPTPRE IKFLSDIDDQ EGLRFQIPVI QFYHKDSSMG
     RKDPVKVIKK AIAETLVFYY PFAGRLREGN GRKLMVDCTG EGIMFVEADA DVTLEQFGDE
     LQPPFPCLEE LLYDVPDSAG VLNCPLLLIQ VTRLRCGGFI FALRLNHTMS DAPGLVQFMT
     AVGEMARGAS APSILPVWCR ELLNARNPPQ VTCTHHEYDE VRDTKGTIIP LDDMVHKSFF
     FGPSEVSALR RFVPHHLRKC STFELLTAVL WRCRTMSLKP DPEEEVRALC IVNARSRFNP
     PLPTGYYGNA FAFPVAVTTA AKLSKNPLGY ALELVKKTKS DVTEEYMKSV ADLMVLKGRP
     HFTVVRTFLV SDVTRGGFGE VDFGWGKAVY GGPAKGGVGA IPGVASFYIP FKNKKGENGI
     VVPICLPGFA METFVKELDG MLKVDAPLDN SNYAIIRPAL