ID   RUVV_FOWPN              Reviewed;         156 AA.
AC   Q9J546;
DT   29-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   02-JUN-2021, entry version 69.
DE   RecName: Full=Holliday junction resolvase;
DE            EC=3.1.-.-;
GN   OrderedLocusNames=FPV187;
OS   Fowlpox virus (strain NVSL) (FPV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Avipoxvirus.
OX   NCBI_TaxID=928301;
OH   NCBI_TaxID=7742; Vertebrata.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=10729156; DOI=10.1128/jvi.74.8.3815-3831.2000;
RA   Afonso C.L., Tulman E.R., Lu Z., Zsak L., Kutish G.F., Rock D.L.;
RT   "The genome of fowlpox virus.";
RL   J. Virol. 74:3815-3831(2000).
CC   -!- FUNCTION: Nuclease that specifically cleaves and resolves four-way DNA
CC       Holliday junctions into linear duplex products. {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the RuvC family. Poxviruses-type subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AF198100; AAF44531.1; -; Genomic_DNA.
DR   RefSeq; NP_039150.1; NC_002188.1.
DR   PDB; 6P7A; X-ray; 3.08 A; A/B=1-148.
DR   PDB; 6P7B; X-ray; 3.32 A; A/B/C/D=1-149.
DR   PDBsum; 6P7A; -.
DR   PDBsum; 6P7B; -.
DR   BMRB; Q9J546; -.
DR   SMR; Q9J546; -.
DR   GeneID; 1486759; -.
DR   KEGG; vg:1486759; -.
DR   Proteomes; UP000008597; Genome.
DR   GO; GO:0008821; F:crossover junction endodeoxyribonuclease activity; IDA:CACAO.
DR   GO; GO:0000400; F:four-way junction DNA binding; IEA:InterPro.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.420.10; -; 1.
DR   InterPro; IPR006932; HJ-resolvase_A22.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF04848; Pox_A22; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA damage; DNA recombination; DNA repair; Hydrolase;
KW   Magnesium; Nuclease; Reference proteome.
FT   CHAIN           1..156
FT                   /note="Holliday junction resolvase"
FT                   /id="PRO_0000183159"
FT   STRAND          2..7
FT                   /evidence="ECO:0007829|PDB:6P7A"
FT   STRAND          10..12
FT                   /evidence="ECO:0007829|PDB:6P7A"
FT   STRAND          14..21
FT                   /evidence="ECO:0007829|PDB:6P7A"
FT   TURN            22..25
FT                   /evidence="ECO:0007829|PDB:6P7A"
FT   STRAND          26..34
FT                   /evidence="ECO:0007829|PDB:6P7A"
FT   STRAND          38..40
FT                   /evidence="ECO:0007829|PDB:6P7B"
FT   HELIX           41..50
FT                   /evidence="ECO:0007829|PDB:6P7A"
FT   STRAND          55..60
FT                   /evidence="ECO:0007829|PDB:6P7A"
FT   STRAND          64..66
FT                   /evidence="ECO:0007829|PDB:6P7B"
FT   HELIX           69..78
FT                   /evidence="ECO:0007829|PDB:6P7A"
FT   TURN            79..81
FT                   /evidence="ECO:0007829|PDB:6P7A"
FT   STRAND          85..89
FT                   /evidence="ECO:0007829|PDB:6P7A"
FT   HELIX           98..112
FT                   /evidence="ECO:0007829|PDB:6P7A"
FT   HELIX           114..117
FT                   /evidence="ECO:0007829|PDB:6P7A"
FT   HELIX           120..126
FT                   /evidence="ECO:0007829|PDB:6P7A"
FT   HELIX           131..146
FT                   /evidence="ECO:0007829|PDB:6P7A"
SQ   SEQUENCE   156 AA;  18154 MW;  C6C9707548BBC331 CRC64;
     MIICSVDIGI KNPAYAIFNY DNTSNTIKLI AIEKSDWTKN WERSVARDLT RYNPDVVILE
     KQGFKSPNSK IIYFIKGFFY NSNTKVIVRN PTFKGGSYRN RKKQSIDVFI QKISEYTDYK
     NDILNKYTKL DDIADSFNLG LSYMESLLKK CKISKD