ID   RUVV_VACCA              Reviewed;         187 AA.
AC   O57229; Q6J396;
DT   07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   07-APR-2021, entry version 77.
DE   RecName: Full=Holliday junction resolvase;
DE            EC=3.1.-.-;
GN   OrderedLocusNames=MVA133R, ACAM3000_MVA_133;
OS   Vaccinia virus (strain Ankara) (VACV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=126794;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Ankara;
RX   PubMed=9601507; DOI=10.1006/viro.1998.9123;
RA   Antoine G., Scheiflinger F., Dorner F., Falkner F.G.;
RT   "The complete genomic sequence of the modified vaccinia Ankara strain:
RT   comparison with other orthopoxviruses.";
RL   Virology 244:365-396(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Isolate Acambis 3000;
RA   Esposito J.J., Frace M., Sammons S.A., Olsen-Rasmussen M.S., Osborne J.,
RA   Khristova M., Wohlhueter R.M.;
RL   Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Nuclease that specifically cleaves and resolves four-way DNA
CC       Holliday junctions into linear duplex products. {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Lipid-anchor
CC       {ECO:0000250}.
CC   -!- PTM: Acylated by palmitic acid group(s). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the RuvC family. Poxviruses-type subfamily.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAT10532.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; U94848; AAB96524.1; -; Genomic_DNA.
DR   EMBL; AY603355; AAT10532.1; ALT_INIT; Genomic_DNA.
DR   PIR; T37409; T37409.
DR   SMR; O57229; -.
DR   Proteomes; UP000159908; Genome.
DR   Proteomes; UP000172909; Genome.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000400; F:four-way junction DNA binding; IEA:InterPro.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   InterPro; IPR006932; HJ-resolvase_A22.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   Pfam; PF04848; Pox_A22; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA recombination; DNA repair; Hydrolase; Lipoprotein;
KW   Magnesium; Membrane; Nuclease; Palmitate.
FT   CHAIN           1..187
FT                   /note="Holliday junction resolvase"
FT                   /id="PRO_0000183155"
SQ   SEQUENCE   187 AA;  21860 MW;  C0D1D1D8B344DF92 CRC64;
     METLTSSSQS LISSPMSKKD YSSEIICAFD IGAKNPARTV LEVKDNSVRV LDISKLDWSS
     DWERHIAKDL SQYEYTTVLL ERQPRRSPYV KFIYFIKGFL YHTSAAKVIC VSPVMSGNSY
     RDRKKRSVEA FLDWMDTFGL RDSVPDRRKL DDVADSFNLA MRYVLDKWNT NYTPYNRCKS
     RNYIKKM