ID   RUVV_VACCC              Reviewed;         176 AA.
AC   P20997;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   23-FEB-2022, entry version 86.
DE   RecName: Full=Resolvase A22;
DE            EC=3.1.-.-;
GN   ORFNames=A22R;
OS   Vaccinia virus (strain Copenhagen) (VACV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10249;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=2219722; DOI=10.1016/0042-6822(90)90294-2;
RA   Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA   Paoletti E.;
RT   "The complete DNA sequence of vaccinia virus.";
RL   Virology 179:247-266(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA   Paoletti E.;
RT   "Appendix to 'The complete DNA sequence of vaccinia virus'.";
RL   Virology 179:517-563(1990).
CC   -!- FUNCTION: Plays a role in DNA replication by cleaving viral DNA
CC       concatamers to yield unit-length viral genomes. The concatamer
CC       junctions contain inverted repeat sequences that can be extruded as
CC       cruciforms, yielding Holliday junctions that A22 protein cleaves (By
CC       similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the RuvC family. Poxviruses-type subfamily.
CC       {ECO:0000305}.
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DR   EMBL; M35027; AAA48146.1; -; Genomic_DNA.
DR   PIR; F42519; F42519.
DR   SMR; P20997; -.
DR   DIP; DIP-2190N; -.
DR   MINT; P20997; -.
DR   Proteomes; UP000008269; Genome.
DR   GO; GO:0000400; F:four-way junction DNA binding; IEA:InterPro.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   InterPro; IPR006932; HJ-resolvase_A22.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   Pfam; PF04848; Pox_A22; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA recombination; DNA repair; Hydrolase; Lipoprotein;
KW   Magnesium; Nuclease; Palmitate; Reference proteome.
FT   CHAIN           1..176
FT                   /note="Resolvase A22"
FT                   /id="PRO_0000183156"
SQ   SEQUENCE   176 AA;  20732 MW;  4EEA5D1B78ED9860 CRC64;
     MSSPMSKKDY SSEIICAFDI GAKNPARTVL EVKDNSVRVL DISKLDWSSD WERRIAKDLS
     QYEYTTVLLE RQPRRSPYVK FIYFIKGFLY HTSAAKVICV SPVMSGNSYR DRKKRSVEAF
     LDWMDTFGLR DSVPDRRKLD DVADSFNLAM RYVLDKWNTN YTPYNRCKSR NYIKKM