ID   RUVV_VACCW              Reviewed;         187 AA.
AC   Q80HV3;
DT   07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   02-JUN-2021, entry version 69.
DE   RecName: Full=Resolvase A22;
DE            EC=3.1.-.-;
DE   AltName: Full=DNA holliday junction resolvase A22;
GN   OrderedLocusNames=VACWR142; ORFNames=A22R;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   PALMITOYLATION.
RX   PubMed=11017799; DOI=10.1006/viro.2000.0522;
RA   Grosenbach D.W., Hansen S.G., Hruby D.E.;
RT   "Identification and analysis of vaccinia virus palmitylproteins.";
RL   Virology 275:193-206(2000).
RN   [3]
RP   FUNCTION.
RX   PubMed=10890916; DOI=10.1073/pnas.150238697;
RA   Garcia A.D., Aravind L., Koonin E.V., Moss B.;
RT   "Bacterial-type DNA holliday junction resolvases in eukaryotic viruses.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:8926-8931(2000).
RN   [4]
RP   FUNCTION.
RX   PubMed=11413313; DOI=10.1128/jvi.75.14.6460-6471.2001;
RA   Garcia A.D., Moss B.;
RT   "Repression of vaccinia virus Holliday junction resolvase inhibits
RT   processing of viral DNA into unit-length genomes.";
RL   J. Virol. 75:6460-6471(2001).
RN   [5]
RP   FUNCTION.
RX   PubMed=16781759; DOI=10.1016/j.virol.2006.05.007;
RA   Culyba M.J., Harrison J.E., Hwang Y., Bushman F.D.;
RT   "DNA cleavage by the A22R resolvase of vaccinia virus.";
RL   Virology 352:466-476(2006).
CC   -!- FUNCTION: Plays a role in DNA replication by cleaving viral DNA
CC       concatamers to yield unit-length viral genomes. The concatamer
CC       junctions contain inverted repeat sequences that can be extruded as
CC       cruciforms, yielding Holliday junctions that A22 protein cleaves.
CC       {ECO:0000269|PubMed:10890916, ECO:0000269|PubMed:11413313,
CC       ECO:0000269|PubMed:16781759}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the RuvC family. Poxviruses-type subfamily.
CC       {ECO:0000305}.
CC   -!- CAUTION: Was initially reported be palmyoylated (PubMed:11017799).
CC       However, additional data are required to confirm this result.
CC       {ECO:0000305|PubMed:11017799}.
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DR   EMBL; AY243312; AAO89421.1; -; Genomic_DNA.
DR   RefSeq; YP_233024.1; NC_006998.1.
DR   SMR; Q80HV3; -.
DR   DNASU; 3707672; -.
DR   GeneID; 3707672; -.
DR   KEGG; vg:3707672; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0000400; F:four-way junction DNA binding; IEA:InterPro.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   InterPro; IPR006932; HJ-resolvase_A22.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   Pfam; PF04848; Pox_A22; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
PE   1: Evidence at protein level;
KW   DNA damage; DNA recombination; DNA repair; Hydrolase; Magnesium; Nuclease;
KW   Reference proteome.
FT   CHAIN           1..187
FT                   /note="Resolvase A22"
FT                   /id="PRO_0000183157"
SQ   SEQUENCE   187 AA;  21879 MW;  D531DE38A944DF92 CRC64;
     METLTSSSQS LISSPMSKKD YSSEIICAFD IGAKNPARTV LEVKDNSVRV LDISKLDWSS
     DWERRIAKDL SQYEYTTVLL ERQPRRSPYV KFIYFIKGFL YHTSAAKVIC VSPVMSGNSY
     RDRKKRSVEA FLDWMDTFGL RDSVPDRRKL DDVADSFNLA MRYVLDKWNT NYTPYNRCKS
     RNYIKKM