ID   RUVV_VAR67              Reviewed;         187 AA.
AC   P0DOS5; P33845;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-OCT-2019, sequence version 1.
DT   12-AUG-2020, entry version 6.
DE   RecName: Full=Resolvase A22;
DE            EC=3.1.-.-;
GN   ORFNames=A22R, A23R;
OS   Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=587200;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8384129; DOI=10.1016/0014-5793(93)80041-r;
RA   Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.;
RT   "Genes of variola and vaccinia viruses necessary to overcome the host
RT   protective mechanisms.";
RL   FEBS Lett. 319:80-83(1993).
CC   -!- FUNCTION: Plays a role in DNA replication by cleaving viral DNA
CC       concatamers to yield unit-length viral genomes. The concatamer
CC       junctions contain inverted repeat sequences that can be extruded as
CC       cruciforms, yielding Holliday junctions that A22 protein cleaves (By
CC       similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the RuvC family. Poxviruses-type subfamily.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X69198; CAA49067.1; -; Genomic_DNA.
DR   PIR; E36850; E36850.
DR   RefSeq; NP_042170.1; NC_001611.1.
DR   SMR; P0DOS5; -.
DR   GeneID; 1486498; -.
DR   KEGG; vg:1486498; -.
DR   Proteomes; UP000002060; Genome.
DR   GO; GO:0000400; F:four-way junction DNA binding; IEA:InterPro.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   InterPro; IPR006932; HJ-resolvase_A22.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   Pfam; PF04848; Pox_A22; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA recombination; DNA repair; Hydrolase; Lipoprotein;
KW   Magnesium; Nuclease; Palmitate; Reference proteome.
FT   CHAIN           1..187
FT                   /note="Resolvase A22"
FT                   /id="PRO_0000183158"
SQ   SEQUENCE   187 AA;  21995 MW;  2F2ADA05D939262F CRC64;
     METLTSSSQS LISSPMSKKD YSSEIICAFD IGAKNPARTV LEVKDNSVRV LDISKLDWSS
     DWERRIAKDL SQYEYTTVLL ERQPRRSPYV KFIYFIKGFL YHTSATKVIC VSPVMSGNSY
     RDRKKRSVEA FFDWMDIFGL RDSVPDRRKL DDVADSFNLA MRYVLDKWNT NYTHYNRCKS
     RNYIKKM