ID   RUVV_VARV               Reviewed;         187 AA.
AC   P0DOS6; P33845;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-OCT-2019, sequence version 1.
DT   29-SEP-2021, entry version 11.
DE   RecName: Full=Resolvase A22;
DE            EC=3.1.-.-;
GN   ORFNames=A22R, A23R;
OS   Variola virus.
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=10255;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Bangladesh-1975;
RX   PubMed=8264798; DOI=10.1038/366748a0;
RA   Massung R.F., Esposito J.J., Liu L.I., Qi J., Utterback T.R., Knight J.C.,
RA   Aubin L., Yuran T.E., Parsons J.M., Loparev V.N., Selivanov N.A.,
RA   Cavallaro K.F., Kerlavage A.R., Mahy B.W.J., Venter J.C.;
RT   "Potential virulence determinants in terminal regions of variola smallpox
RT   virus genome.";
RL   Nature 366:748-751(1993).
CC   -!- FUNCTION: Plays a role in DNA replication by cleaving viral DNA
CC       concatamers to yield unit-length viral genomes. The concatamer
CC       junctions contain inverted repeat sequences that can be extruded as
CC       cruciforms, yielding Holliday junctions that A22 protein cleaves (By
CC       similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the RuvC family. Poxviruses-type subfamily.
CC       {ECO:0000305}.
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DR   EMBL; L22579; AAA60874.1; -; Genomic_DNA.
DR   PIR; T28564; T28564.
DR   RefSeq; NP_042170.1; NC_001611.1.
DR   SMR; P0DOS6; -.
DR   GeneID; 1486498; -.
DR   KEGG; vg:1486498; -.
DR   Proteomes; UP000119805; Genome.
DR   GO; GO:0000400; F:four-way junction DNA binding; IEA:InterPro.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   InterPro; IPR006932; HJ-resolvase_A22.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   Pfam; PF04848; Pox_A22; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA recombination; DNA repair; Hydrolase; Lipoprotein;
KW   Magnesium; Nuclease; Palmitate.
FT   CHAIN           1..187
FT                   /note="Resolvase A22"
FT                   /id="PRO_0000448124"
SQ   SEQUENCE   187 AA;  21995 MW;  2F2ADA05D939262F CRC64;
     METLTSSSQS LISSPMSKKD YSSEIICAFD IGAKNPARTV LEVKDNSVRV LDISKLDWSS
     DWERRIAKDL SQYEYTTVLL ERQPRRSPYV KFIYFIKGFL YHTSATKVIC VSPVMSGNSY
     RDRKKRSVEA FFDWMDIFGL RDSVPDRRKL DDVADSFNLA MRYVLDKWNT NYTHYNRCKS
     RNYIKKM