RUX_DROME
ID RUX_DROME Reviewed; 335 AA.
AC P50445; Q540W5; Q9W446;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 2.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Cell cycle negative regulator roughex;
GN Name=rux; ORFNames=CG4336;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RC TISSUE=Eye imaginal disk;
RX PubMed=8020091; DOI=10.1016/0092-8674(94)90440-5;
RA Thomas B.J., Gunning D.A., Cho J., Zipursky S.L.;
RT "Cell cycle progression in the developing Drosophila eye: roughex encodes a
RT novel protein required for the establishment of G1.";
RL Cell 77:1003-1014(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Negative regulator of both mitosis and meiosis. Required for
CC the establishment of the G1 phase in the developing eye.
CC {ECO:0000269|PubMed:8020091}.
CC -!- DISRUPTION PHENOTYPE: Rough eye phenotype, and/or male sterility and
CC in, some cases, pupal death. {ECO:0000269|PubMed:8020091}.
CC -!- MISCELLANEOUS: The rux phenotype is suppressed by mutations of genes
CC involved in cell cycle progression such as CycA and string, and
CC enhanced by mutations of intercellular signaling genes, Ras1 and Star.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U19583; AAA61580.1; -; mRNA.
DR EMBL; AE014298; AAF46113.1; -; Genomic_DNA.
DR EMBL; AY119537; AAM50191.1; -; mRNA.
DR PIR; A54458; A54458.
DR RefSeq; NP_001245540.1; NM_001258611.1.
DR RefSeq; NP_511053.2; NM_078498.4.
DR AlphaFoldDB; P50445; -.
DR BioGRID; 58031; 57.
DR DIP; DIP-23711N; -.
DR ELM; P50445; -.
DR IntAct; P50445; 39.
DR STRING; 7227.FBpp0298289; -.
DR PaxDb; P50445; -.
DR DNASU; 31535; -.
DR EnsemblMetazoa; FBtr0070889; FBpp0070854; FBgn0003302.
DR EnsemblMetazoa; FBtr0307288; FBpp0298289; FBgn0003302.
DR GeneID; 31535; -.
DR KEGG; dme:Dmel_CG4336; -.
DR UCSC; CG4336-RA; d. melanogaster.
DR CTD; 31535; -.
DR FlyBase; FBgn0003302; rux.
DR VEuPathDB; VectorBase:FBgn0003302; -.
DR eggNOG; ENOG502TCUG; Eukaryota.
DR HOGENOM; CLU_829681_0_0_1; -.
DR InParanoid; P50445; -.
DR OMA; PRKRQHK; -.
DR OrthoDB; 1472719at2759; -.
DR PhylomeDB; P50445; -.
DR BioGRID-ORCS; 31535; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 31535; -.
DR PRO; PR:P50445; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0003302; Expressed in testis and 28 other tissues.
DR ExpressionAtlas; P50445; baseline and differential.
DR Genevisible; P50445; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0000278; P:mitotic cell cycle; IMP:FlyBase.
DR GO; GO:0051726; P:regulation of cell cycle; IMP:FlyBase.
DR GO; GO:0007096; P:regulation of exit from mitosis; TAS:FlyBase.
DR GO; GO:0040020; P:regulation of meiotic nuclear division; TAS:FlyBase.
DR InterPro; IPR009259; Roughex.
DR Pfam; PF06020; Roughex; 1.
PE 2: Evidence at transcript level;
KW Reference proteome.
FT CHAIN 1..335
FT /note="Cell cycle negative regulator roughex"
FT /id="PRO_0000097536"
FT REGION 222..291
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 304..335
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 237..265
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 304..322
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 328
FT /note="T -> M (in Ref. 1; AAA61580)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 335 AA; 37898 MW; 0A48CECB08C8774F CRC64;
MSAPEEHKET PLEVIHEFIN GVDDGTIRRD LGEDCILSFY SRNVRGAKAI TGFLRNQLTM
RYKHEGFEEA AQIEWGDELL LKARFGRSFD SERRRIYEEK ERTGTTLHVR PESDDEEVNE
KFSSTLITPP RPSSYNLNSL KYVEACGLLN KRNEHIYGGL DMGESCAVHL TLGYRSTFLP
GGQVSGFEIC LAVYDRGLTS LKRSTLIPPL CSISFARRAS ARCNPTTDDE EDTEEDSTPP
TARRCVRRTL FTEENTQKEE DADFIPVVEQ EQPAPQEQPA PQQAEETGEV VIPVDISTAL
KTTNFSSCTP RKRQQATNGN EVVRKRATGP RRMRF
