RV203_MYCTU
ID RV203_MYCTU Reviewed; 136 AA.
AC I6X8R5;
DT 29-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2012, sequence version 1.
DT 03-AUG-2022, entry version 48.
DE RecName: Full=Heme-binding protein Rv0203 {ECO:0000305};
DE Flags: Precursor;
GN OrderedLocusNames=Rv0203 {ECO:0000312|EMBL:CCP42931.1},
GN RVBD_0203 {ECO:0000312|EMBL:AFN48054.1};
GN ORFNames=P425_00212 {ECO:0000312|EMBL:KBJ41312.1};
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RG The Broad Institute Genome Sequencing Platform;
RA Galagan J., Kreiswirth B., Dobos K., Fortune S., Fitzgerald M., Young S.K.,
RA Zeng Q., Gargeya S., Abouelleil A., Alvarado L., Berlin A.M., Chapman S.B.,
RA Gainer-Dewar J., Goldberg J., Gnerre S., Griggs A., Gujja S., Hansen M.,
RA Howarth C., Imamovic A., Larimer J., McCowan C., Murphy C., Pearson M.,
RA Poon T., Priest M., Roberts A., Saif S., Shea T., Sykes S., Wortman J.,
RA Nusbaum C., Birren B.;
RT "The genome sequence of Mycobacterium tuberculosis H37Rv.";
RL Submitted (NOV-2013) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RG The Broad Institute Genomics Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Earl A.M., Kreiswirth B., Gomez J., Victor T., Desjardins C., Abeel T.,
RA Young S., Zeng Q., Gargeya S., Abouelleil A., Alvarado L., Chapman S.B.,
RA Gainer-Dewar J., Goldberg J., Griggs A., Gujja S., Hansen M., Howarth C.,
RA Imamovic A., Larimer J., Murphy C., Naylor J., Pearson M., Poon T.W.,
RA Priest M., Roberts A., Saif S., Shea T., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The genome sequence of Mycobacterium tuberculosis H37Rv.";
RL Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN [5]
RP FUNCTION, HEME BINDING, AND MUTAGENESIS OF TYR-60; HIS-64 AND HIS-90.
RX PubMed=22283334; DOI=10.1021/bi2018305;
RA Owens C.P., Du J., Dawson J.H., Goulding C.W.;
RT "Characterization of heme ligation properties of Rv0203, a secreted heme
RT binding protein involved in Mycobacterium tuberculosis heme uptake.";
RL Biochemistry 51:1518-1531(2012).
RN [6]
RP FUNCTION, AND HEME BINDING.
RX PubMed=23760277; DOI=10.1074/jbc.m113.453076;
RA Owens C.P., Chim N., Graves A.B., Harmston C.A., Iniguez A., Contreras H.,
RA Liptak M.D., Goulding C.W.;
RT "The Mycobacterium tuberculosis secreted protein Rv0203 transfers heme to
RT membrane proteins MmpL3 and MmpL11.";
RL J. Biol. Chem. 288:21714-21728(2013).
RN [7]
RP X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 36-136, FUNCTION, HEME BINDING,
RP SUBUNIT, SUBCELLULAR LOCATION, DISULFIDE BOND, AND MUTAGENESIS OF TYR-60.
RC STRAIN=H37Rv;
RX PubMed=21383189; DOI=10.1073/pnas.1009516108;
RA Tullius M.V., Harmston C.A., Owens C.P., Chim N., Morse R.P., McMath L.M.,
RA Iniguez A., Kimmey J.M., Sawaya M.R., Whitelegge J.P., Horwitz M.A.,
RA Goulding C.W.;
RT "Discovery and characterization of a unique mycobacterial heme acquisition
RT system.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:5051-5056(2011).
CC -!- FUNCTION: Part of a heme-iron acquisition system. Acts by binding heme
CC and delivering it to the membrane proteins MmpL3 and MmpL11. Can use
CC free heme or heme from host hemoglobin. {ECO:0000269|PubMed:21383189,
CC ECO:0000269|PubMed:22283334, ECO:0000269|PubMed:23760277}.
CC -!- SUBUNIT: Dimer of dimers. {ECO:0000269|PubMed:21383189}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:21383189}.
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DR EMBL; AL123456; CCP42931.1; -; Genomic_DNA.
DR EMBL; CP003248; AFN48054.1; -; Genomic_DNA.
DR EMBL; JLDD01000001; KBJ41312.1; -; Genomic_DNA.
DR RefSeq; NP_214717.1; NC_000962.3.
DR RefSeq; WP_003401194.1; NZ_NVQJ01000001.1.
DR PDB; 3MAY; X-ray; 2.50 A; A/B/C/D/E/F/G/H=36-136.
DR PDBsum; 3MAY; -.
DR AlphaFoldDB; I6X8R5; -.
DR SMR; I6X8R5; -.
DR STRING; 83332.Rv0203; -.
DR PaxDb; I6X8R5; -.
DR GeneID; 45424174; -.
DR GeneID; 886748; -.
DR KEGG; mtu:Rv0203; -.
DR KEGG; mtv:RVBD_0203; -.
DR PATRIC; fig|83332.111.peg.233; -.
DR TubercuList; Rv0203; -.
DR eggNOG; ENOG5031M5S; Bacteria.
DR OMA; DMQGLAN; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0015886; P:heme transport; IEA:InterPro.
DR Gene3D; 1.20.20.20; -; 1.
DR InterPro; IPR030937; Hemophore_Rv0203.
DR InterPro; IPR032407; MHB.
DR InterPro; IPR038378; MHB_sf.
DR Pfam; PF16525; MHB; 1.
DR TIGRFAMs; TIGR04530; hemophoreRv0203; 1.
DR TIGRFAMs; TIGR04529; MTB_hemophore; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Disulfide bond; Heme; Iron; Metal-binding;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..136
FT /note="Heme-binding protein Rv0203"
FT /id="PRO_0000432886"
FT BINDING 60
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000305|PubMed:21383189,
FT ECO:0000305|PubMed:22283334"
FT BINDING 64
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /evidence="ECO:0000305|PubMed:22283334"
FT BINDING 90
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /evidence="ECO:0000305|PubMed:22283334"
FT DISULFID 41..115
FT /evidence="ECO:0000269|PubMed:21383189"
FT MUTAGEN 60
FT /note="Y->A: Does not bind heme."
FT /evidence="ECO:0000269|PubMed:21383189,
FT ECO:0000269|PubMed:22283334"
FT MUTAGEN 60
FT /note="Y->F,H: Decrease in heme binding."
FT /evidence="ECO:0000269|PubMed:22283334"
FT MUTAGEN 64
FT /note="H->A: Decrease in heme binding. Strong decrease in
FT heme binding; when associated with A-90."
FT /evidence="ECO:0000269|PubMed:22283334"
FT MUTAGEN 90
FT /note="H->A: Decrease in heme binding. Strong decrease in
FT heme binding; when associated with A-64."
FT /evidence="ECO:0000269|PubMed:22283334"
SQ SEQUENCE 136 AA; 13901 MW; 3B9EE7B126A6239B CRC64;
MKTGTATTRR RLLAVLIALA LPGAAVALLA EPSATGASDP CAASEVARTV GSVAKSMGDY
LDSHPETNQV MTAVLQQQVG PGSVASLKAH FEANPKVASD LHALSQPLTD LSTRCSLPIS
GLQAIGLMQA VQGARR
