RWD2A_HUMAN
ID RWD2A_HUMAN Reviewed; 292 AA.
AC Q9UIY3; B4DIQ3; E1P548; Q2M3R3; Q96FH1;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=RWD domain-containing protein 2A;
GN Name=RWDD2A; Synonyms=RWDD2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Hippocampus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=14574404; DOI=10.1038/nature02055;
RA Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA Rogers J., Beck S.;
RT "The DNA sequence and analysis of human chromosome 6.";
RL Nature 425:805-811(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Bone marrow, and Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP STRUCTURE BY NMR OF 1-141.
RG RIKEN structural genomics initiative (RSGI);
RT "Solution structure of the RWD domain of human RWD domain containing
RT protein 2.";
RL Submitted (JUN-2006) to the PDB data bank.
CC -!- INTERACTION:
CC Q9UIY3; Q15052: ARHGEF6; NbExp=3; IntAct=EBI-17677006, EBI-1642523;
CC Q9UIY3; Q9BTE3-2: MCMBP; NbExp=3; IntAct=EBI-17677006, EBI-9384556;
CC Q9UIY3; P20618: PSMB1; NbExp=3; IntAct=EBI-17677006, EBI-372273;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9UIY3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9UIY3-2; Sequence=VSP_055504;
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK295724; BAG58565.1; -; mRNA.
DR EMBL; AL049699; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471051; EAW48665.1; -; Genomic_DNA.
DR EMBL; CH471051; EAW48666.1; -; Genomic_DNA.
DR EMBL; BC010930; AAH10930.1; -; mRNA.
DR EMBL; BC104816; AAI04817.1; -; mRNA.
DR EMBL; BC112003; AAI12004.1; -; mRNA.
DR CCDS; CCDS4998.1; -. [Q9UIY3-1]
DR RefSeq; NP_001309264.1; NM_001322335.1.
DR RefSeq; NP_001309265.1; NM_001322336.1.
DR RefSeq; NP_001309266.1; NM_001322337.1.
DR RefSeq; NP_001309268.1; NM_001322339.1.
DR RefSeq; NP_219479.2; NM_033411.4. [Q9UIY3-1]
DR PDB; 2DAW; NMR; -; A=1-141.
DR PDBsum; 2DAW; -.
DR AlphaFoldDB; Q9UIY3; -.
DR BMRB; Q9UIY3; -.
DR SMR; Q9UIY3; -.
DR BioGRID; 125196; 8.
DR IntAct; Q9UIY3; 5.
DR STRING; 9606.ENSP00000358739; -.
DR iPTMnet; Q9UIY3; -.
DR PhosphoSitePlus; Q9UIY3; -.
DR BioMuta; RWDD2A; -.
DR DMDM; 9979483; -.
DR EPD; Q9UIY3; -.
DR MassIVE; Q9UIY3; -.
DR MaxQB; Q9UIY3; -.
DR PaxDb; Q9UIY3; -.
DR PeptideAtlas; Q9UIY3; -.
DR PRIDE; Q9UIY3; -.
DR ProteomicsDB; 4318; -.
DR ProteomicsDB; 84576; -. [Q9UIY3-1]
DR Antibodypedia; 31673; 68 antibodies from 16 providers.
DR DNASU; 112611; -.
DR Ensembl; ENST00000369724.5; ENSP00000358739.3; ENSG00000013392.8. [Q9UIY3-1]
DR GeneID; 112611; -.
DR KEGG; hsa:112611; -.
DR MANE-Select; ENST00000369724.5; ENSP00000358739.3; NM_033411.5; NP_219479.2.
DR UCSC; uc003pjx.5; human. [Q9UIY3-1]
DR CTD; 112611; -.
DR GeneCards; RWDD2A; -.
DR HGNC; HGNC:21385; RWDD2A.
DR HPA; ENSG00000013392; Low tissue specificity.
DR neXtProt; NX_Q9UIY3; -.
DR OpenTargets; ENSG00000013392; -.
DR PharmGKB; PA162402307; -.
DR VEuPathDB; HostDB:ENSG00000013392; -.
DR eggNOG; ENOG502QTUP; Eukaryota.
DR GeneTree; ENSGT00390000007224; -.
DR HOGENOM; CLU_046295_1_0_1; -.
DR InParanoid; Q9UIY3; -.
DR OMA; MFPNKGE; -.
DR PhylomeDB; Q9UIY3; -.
DR TreeFam; TF324344; -.
DR PathwayCommons; Q9UIY3; -.
DR SignaLink; Q9UIY3; -.
DR BioGRID-ORCS; 112611; 10 hits in 1077 CRISPR screens.
DR ChiTaRS; RWDD2A; human.
DR EvolutionaryTrace; Q9UIY3; -.
DR GenomeRNAi; 112611; -.
DR Pharos; Q9UIY3; Tdark.
DR PRO; PR:Q9UIY3; -.
DR Proteomes; UP000005640; Chromosome 6.
DR RNAct; Q9UIY3; protein.
DR Bgee; ENSG00000013392; Expressed in endothelial cell and 145 other tissues.
DR Genevisible; Q9UIY3; HS.
DR Gene3D; 3.10.110.10; -; 1.
DR InterPro; IPR010541; Prp3_C.
DR InterPro; IPR006575; RWD-domain.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR InterPro; IPR017359; UCP038021_RWD.
DR PANTHER; PTHR15955; PTHR15955; 1.
DR Pfam; PF06544; DUF1115; 1.
DR Pfam; PF05773; RWD; 1.
DR PIRSF; PIRSF038021; UCP038021_RWDD2; 1.
DR SMART; SM00591; RWD; 1.
DR SUPFAM; SSF54495; SSF54495; 1.
DR PROSITE; PS50908; RWD; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Reference proteome.
FT CHAIN 1..292
FT /note="RWD domain-containing protein 2A"
FT /id="PRO_0000097543"
FT DOMAIN 14..134
FT /note="RWD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00179"
FT VAR_SEQ 1..66
FT /note="MSASVKESLQLQLLEMEMLFSMFPNQGEVKLEDVNALTNIKRYLEGTREALP
FT PKIEFVITLQIEEP -> MLWYLNLFDFHF (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_055504"
FT CONFLICT 83
FT /note="V -> L (in Ref. 4; AAH10930)"
FT /evidence="ECO:0000305"
FT HELIX 5..22
FT /evidence="ECO:0007829|PDB:2DAW"
FT STRAND 28..32
FT /evidence="ECO:0007829|PDB:2DAW"
FT HELIX 34..36
FT /evidence="ECO:0007829|PDB:2DAW"
FT HELIX 38..45
FT /evidence="ECO:0007829|PDB:2DAW"
FT STRAND 55..63
FT /evidence="ECO:0007829|PDB:2DAW"
FT TURN 64..67
FT /evidence="ECO:0007829|PDB:2DAW"
FT STRAND 68..76
FT /evidence="ECO:0007829|PDB:2DAW"
FT STRAND 86..90
FT /evidence="ECO:0007829|PDB:2DAW"
FT STRAND 92..94
FT /evidence="ECO:0007829|PDB:2DAW"
FT HELIX 96..110
FT /evidence="ECO:0007829|PDB:2DAW"
FT HELIX 120..129
FT /evidence="ECO:0007829|PDB:2DAW"
FT HELIX 132..135
FT /evidence="ECO:0007829|PDB:2DAW"
SQ SEQUENCE 292 AA; 33893 MW; 00669FA229F0D487 CRC64;
MSASVKESLQ LQLLEMEMLF SMFPNQGEVK LEDVNALTNI KRYLEGTREA LPPKIEFVIT
LQIEEPKVKI DLQVTMPHSY PYVALQLFGR SSELDRHQQL LLNKGLTSYI GTFDPGELCV
CAAIQWLQDN SASYFLNRKL VYEPSTQAKP VKNTFLRMWI YSHHIYQQDL RKKILDVGKR
LDVTGFCMTG KPGIICVEGF KEHCEEFWHT IRYPNWKHIS CKHAESVETE GNGEDLRLFH
SFEELLLEAH GDYGLRNDYH MNLGQFLEFL KKHKSEHVFQ ILFGIESKSS DS
