RWD2B_HUMAN
ID RWD2B_HUMAN Reviewed; 319 AA.
AC P57060;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 1.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=RWD domain-containing protein 2B;
GN Name=RWDD2B; Synonyms=C21orf6; ORFNames=GL011;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=10729227; DOI=10.1006/geno.1999.6109;
RA Orti R., Rachidi M., Vialard F., Toyama K., Lopes C., Taudien S.,
RA Rosenthal A., Yaspo M.-L., Sinet P.-M., Delabar J.-M.;
RT "Characterization of a novel gene, C21orf6, mapping to a critical region of
RT chromosome 21q22.1 involved in the monosomy 21 phenotype and of its murine
RT ortholog, orf5.";
RL Genomics 64:203-210(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Liver;
RA Li Y., Wu T., Xu S., Ren S., Chen Z., Han Z.;
RT "A novel gene expressed in human liver non-tumor tissues.";
RL Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Colon;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=10830953; DOI=10.1038/35012518;
RA Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S.,
RA Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M.,
RA Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A., Menzel U.,
RA Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A.,
RA Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J.,
RA Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K.,
RA Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G.,
RA Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J.,
RA Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S.,
RA Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K.,
RA Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L.;
RT "The DNA sequence of human chromosome 21.";
RL Nature 405:311-319(2000).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Skeletal muscle;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full phosphorylation
RT site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma, and Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-275, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
RN [10]
RP STRUCTURE BY NMR OF 34-173.
RG RIKEN structural genomics initiative (RSGI);
RT "Solution structure of the RWD domain of human protein C21orf6.";
RL Submitted (JUN-2006) to the PDB data bank.
CC -!- INTERACTION:
CC P57060; Q92997: DVL3; NbExp=3; IntAct=EBI-724442, EBI-739789;
CC P57060; P11171-2: EPB41; NbExp=3; IntAct=EBI-724442, EBI-10197451;
CC P57060; Q96GL9: FAM163A; NbExp=3; IntAct=EBI-724442, EBI-11793142;
CC P57060; O95257: GADD45G; NbExp=15; IntAct=EBI-724442, EBI-448202;
CC P57060; Q13422: IKZF1; NbExp=4; IntAct=EBI-724442, EBI-745305;
CC P57060; Q8N8K9: KIAA1958; NbExp=3; IntAct=EBI-724442, EBI-10181113;
CC P57060; Q6A162: KRT40; NbExp=6; IntAct=EBI-724442, EBI-10171697;
CC P57060; P60409: KRTAP10-7; NbExp=3; IntAct=EBI-724442, EBI-10172290;
CC P57060; Q6FHY5: MEOX2; NbExp=3; IntAct=EBI-724442, EBI-16439278;
CC P57060; Q8IXK0: PHC2; NbExp=3; IntAct=EBI-724442, EBI-713786;
CC P57060; Q9C029: TRIM7; NbExp=3; IntAct=EBI-724442, EBI-2813981;
CC -!- TISSUE SPECIFICITY: Ubiquitous.
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DR EMBL; Y19009; CAB88085.1; -; mRNA.
DR EMBL; AF212232; AAK14918.1; -; mRNA.
DR EMBL; AK000056; BAA90913.1; -; mRNA.
DR EMBL; AL163249; CAB90431.1; -; Genomic_DNA.
DR EMBL; BC012546; AAH12546.1; -; mRNA.
DR EMBL; BC017912; AAH17912.1; -; mRNA.
DR CCDS; CCDS13582.1; -.
DR RefSeq; NP_058636.1; NM_016940.2.
DR PDB; 2DAX; NMR; -; A=34-172.
DR PDBsum; 2DAX; -.
DR AlphaFoldDB; P57060; -.
DR BMRB; P57060; -.
DR SMR; P57060; -.
DR BioGRID; 115380; 52.
DR IntAct; P57060; 23.
DR MINT; P57060; -.
DR STRING; 9606.ENSP00000418693; -.
DR iPTMnet; P57060; -.
DR PhosphoSitePlus; P57060; -.
DR BioMuta; RWDD2B; -.
DR DMDM; 9972769; -.
DR EPD; P57060; -.
DR jPOST; P57060; -.
DR MassIVE; P57060; -.
DR MaxQB; P57060; -.
DR PaxDb; P57060; -.
DR PeptideAtlas; P57060; -.
DR PRIDE; P57060; -.
DR ProteomicsDB; 56979; -.
DR Antibodypedia; 6351; 12 antibodies from 9 providers.
DR DNASU; 10069; -.
DR Ensembl; ENST00000493196.2; ENSP00000418693.1; ENSG00000156253.7.
DR GeneID; 10069; -.
DR KEGG; hsa:10069; -.
DR MANE-Select; ENST00000493196.2; ENSP00000418693.1; NM_016940.3; NP_058636.1.
DR UCSC; uc002yms.4; human.
DR CTD; 10069; -.
DR DisGeNET; 10069; -.
DR GeneCards; RWDD2B; -.
DR HGNC; HGNC:1302; RWDD2B.
DR HPA; ENSG00000156253; Low tissue specificity.
DR MIM; 617843; gene.
DR neXtProt; NX_P57060; -.
DR OpenTargets; ENSG00000156253; -.
DR PharmGKB; PA162402318; -.
DR VEuPathDB; HostDB:ENSG00000156253; -.
DR eggNOG; ENOG502QR2G; Eukaryota.
DR GeneTree; ENSGT00390000007224; -.
DR HOGENOM; CLU_046295_1_0_1; -.
DR InParanoid; P57060; -.
DR OMA; SMFPYEE; -.
DR OrthoDB; 1387276at2759; -.
DR PhylomeDB; P57060; -.
DR TreeFam; TF324344; -.
DR PathwayCommons; P57060; -.
DR SignaLink; P57060; -.
DR BioGRID-ORCS; 10069; 8 hits in 1082 CRISPR screens.
DR EvolutionaryTrace; P57060; -.
DR GeneWiki; RWDD2B; -.
DR GenomeRNAi; 10069; -.
DR Pharos; P57060; Tdark.
DR PRO; PR:P57060; -.
DR Proteomes; UP000005640; Chromosome 21.
DR RNAct; P57060; protein.
DR Bgee; ENSG00000156253; Expressed in skeletal muscle tissue of biceps brachii and 173 other tissues.
DR Genevisible; P57060; HS.
DR Gene3D; 3.10.110.10; -; 1.
DR InterPro; IPR010541; Prp3_C.
DR InterPro; IPR006575; RWD-domain.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR InterPro; IPR017359; UCP038021_RWD.
DR PANTHER; PTHR15955; PTHR15955; 1.
DR Pfam; PF06544; DUF1115; 1.
DR Pfam; PF05773; RWD; 1.
DR PIRSF; PIRSF038021; UCP038021_RWDD2; 1.
DR SMART; SM00591; RWD; 1.
DR SUPFAM; SSF54495; SSF54495; 1.
DR PROSITE; PS50908; RWD; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Phosphoprotein; Reference proteome.
FT CHAIN 1..319
FT /note="RWD domain-containing protein 2B"
FT /id="PRO_0000079504"
FT DOMAIN 41..165
FT /note="RWD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00179"
FT MOD_RES 275
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT HELIX 34..49
FT /evidence="ECO:0007829|PDB:2DAX"
FT STRAND 52..59
FT /evidence="ECO:0007829|PDB:2DAX"
FT HELIX 61..73
FT /evidence="ECO:0007829|PDB:2DAX"
FT STRAND 83..92
FT /evidence="ECO:0007829|PDB:2DAX"
FT STRAND 98..107
FT /evidence="ECO:0007829|PDB:2DAX"
FT STRAND 119..121
FT /evidence="ECO:0007829|PDB:2DAX"
FT HELIX 127..143
FT /evidence="ECO:0007829|PDB:2DAX"
FT HELIX 152..165
FT /evidence="ECO:0007829|PDB:2DAX"
SQ SEQUENCE 319 AA; 36332 MW; 1E04418893C889B4 CRC64;
MKIELSMQPW NPGYSSEGAT AQETYTCPKM IEMEQAEAQL AELDLLASMF PGENELIVND
QLAVAELKDC IEKKTMEGRS SKVYFTINMN LDVSDEKMAM FSLACILPFK YPAVLPEITV
RSVLLSRSQQ TQLNTDLTAF LQKHCHGDVC ILNATEWVRE HASGYVSRDT SSSPTTGSTV
QSVDLIFTRL WIYSHHIYNK CKRKNILEWA KELSLSGFSM PGKPGVVCVE GPQSACEEFW
SRLRKLNWKR ILIRHREDIP FDGTNDETER QRKFSIFEEK VFSVNGARGN HMDFGQLYQF
LNTKGCGDVF QMFFGVEGQ
