ID   RWDD3_DANRE             Reviewed;         257 AA.
AC   Q6PBV4;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=RWD domain-containing protein 3;
GN   Name=rwdd3; ORFNames=zgc:73229;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Eye;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Enhancer of SUMO conjugation. Increases SUMO conjugation to
CC       proteins by promoting the: binding of E1 and E2 enzymes, thioester
CC       linkage between SUMO and ube2i/ubc9 and transfer of SUMO to specific
CC       target proteins which include hif1a, pias, nfkbia, nr3c1 and top1. Has
CC       no effect on ubiquitination (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Colocalizes with
CC       ubc9/ube2i in nuclear spots. {ECO:0000250}.
CC   -!- DOMAIN: The RWD domain is required for the sumoylation enhancement
CC       activity. {ECO:0000250}.
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DR   EMBL; BC059571; AAH59571.1; -; mRNA.
DR   RefSeq; NP_957050.1; NM_200756.1.
DR   AlphaFoldDB; Q6PBV4; -.
DR   SMR; Q6PBV4; -.
DR   DNASU; 393729; -.
DR   GeneID; 393729; -.
DR   KEGG; dre:393729; -.
DR   CTD; 25950; -.
DR   ZFIN; ZDB-GENE-040426-1723; rwdd3.
DR   InParanoid; Q6PBV4; -.
DR   OrthoDB; 1437243at2759; -.
DR   PhylomeDB; Q6PBV4; -.
DR   Reactome; R-DRE-3065678; SUMO is transferred from E1 to E2 (UBE2I, UBC9).
DR   PRO; PR:Q6PBV4; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0032088; P:negative regulation of NF-kappaB transcription factor activity; IEA:InterPro.
DR   GO; GO:1902073; P:positive regulation of hypoxia-inducible factor-1alpha signaling pathway; IEA:InterPro.
DR   GO; GO:0033235; P:positive regulation of protein sumoylation; IBA:GO_Central.
DR   Gene3D; 3.10.110.10; -; 1.
DR   InterPro; IPR006575; RWD-domain.
DR   InterPro; IPR038840; RWDD3.
DR   InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR   PANTHER; PTHR15628; PTHR15628; 1.
DR   Pfam; PF05773; RWD; 1.
DR   SMART; SM00591; RWD; 1.
DR   SUPFAM; SSF54495; SSF54495; 1.
DR   PROSITE; PS50908; RWD; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Nucleus; Reference proteome.
FT   CHAIN           1..257
FT                   /note="RWD domain-containing protein 3"
FT                   /id="PRO_0000339464"
FT   DOMAIN          7..113
FT                   /note="RWD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00179"
SQ   SEQUENCE   257 AA;  29655 MW;  7B80CABD94965EC8 CRC64;
     MSKEAFDELS VLSAIYCEQG EFEVLEESPE KGVVFRVHTL IDNNEKTPLD IIFHISPEYP
     NTPPDISISS NHLSRRQCHD LRRSLLDTAQ SLPAEPMVHG LMLWLQENFS DLIKTSSCHS
     AEVRPETTEE TWTALLHLDH MRSKAKYIKL IEKWTLELRL TGRLFTGKLI LILLQGTKEN
     IKQYIHLLKS VKVDVDSSGK RCKEKMMSVL CEIPKPEDKI MMTTFEVKDI LSLDDLRREF
     DLIGLNELYH QFVSSLT