ID   RWDD3_PONAB             Reviewed;         267 AA.
AC   Q5RDC7;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   25-MAY-2022, entry version 55.
DE   RecName: Full=RWD domain-containing protein 3;
GN   Name=RWDD3;
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney;
RG   The German cDNA consortium;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Enhancer of SUMO conjugation. Via its interaction with
CC       UBE2I/UBC9, increases SUMO conjugation to proteins by promoting the:
CC       binding of E1 and E2 enzymes, thioester linkage between SUMO and
CC       UBE2I/UBC9 and transfer of SUMO to specific target proteins which
CC       include HIF1A, PIAS, NFKBIA, NR3C1 and TOP1. Positively regulates the
CC       NF-kappa-B signaling pathway by enhancing the sumoylation of NF-kappa-B
CC       inhibitor alpha (NFKBIA), promoting its stabilization which
CC       consequently leads to an increased inhibition of NF-kappa-B
CC       transcriptional activity. Negatively regulates the hypoxia-inducible
CC       factor-1 alpha (HIF1A) signaling pathway by increasing the sumoylation
CC       of HIF1A, promoting its stabilization, transcriptional activity and the
CC       expression of its target gene VEGFA during hypoxia. Has no effect on
CC       ubiquitination (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with UBE2I/UBC9, NFKBIA, HIF1A and NCOA2.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Colocalizes with
CC       UBC9/UBE2I in nuclear spots. {ECO:0000250}.
CC   -!- DOMAIN: The RWD domain is required for the sumoylation enhancement
CC       activity. {ECO:0000250}.
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DR   EMBL; CR857987; CAH90230.1; -; mRNA.
DR   RefSeq; NP_001125096.1; NM_001131624.2.
DR   AlphaFoldDB; Q5RDC7; -.
DR   SMR; Q5RDC7; -.
DR   STRING; 9601.ENSPPYP00000001313; -.
DR   GeneID; 100171978; -.
DR   KEGG; pon:100171978; -.
DR   CTD; 25950; -.
DR   eggNOG; ENOG502QSYH; Eukaryota.
DR   InParanoid; Q5RDC7; -.
DR   OrthoDB; 1437243at2759; -.
DR   Proteomes; UP000001595; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0032088; P:negative regulation of NF-kappaB transcription factor activity; ISS:UniProtKB.
DR   GO; GO:1902073; P:positive regulation of hypoxia-inducible factor-1alpha signaling pathway; ISS:UniProtKB.
DR   GO; GO:0033235; P:positive regulation of protein sumoylation; ISS:UniProtKB.
DR   Gene3D; 3.10.110.10; -; 1.
DR   InterPro; IPR006575; RWD-domain.
DR   InterPro; IPR038840; RWDD3.
DR   InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR   PANTHER; PTHR15628; PTHR15628; 1.
DR   Pfam; PF05773; RWD; 1.
DR   SMART; SM00591; RWD; 1.
DR   SUPFAM; SSF54495; SSF54495; 1.
DR   PROSITE; PS50908; RWD; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Nucleus; Reference proteome.
FT   CHAIN           1..267
FT                   /note="RWD domain-containing protein 3"
FT                   /id="PRO_0000339463"
FT   DOMAIN          7..114
FT                   /note="RWD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00179"
SQ   SEQUENCE   267 AA;  30435 MW;  473D4FB02CE1F1DD CRC64;
     MAEPVQEELS ALAAIFCGPH EWEVLSRSET DGTVFRIHTK AEGFMDADIP LESVFHLPVN
     YPSCLPGISI NSEQLTRAQC VTVKEKLLEQ AESLLSEPMV HELVLWIQQN LRHILNQPET
     GSGYEKCTFS TSTTVDDGLW ITLLHLDHMR AKTKYVKTVE KWASDLRLTG RLMFMGKIIL
     ILLQGDRNNL KEYLILQKTS KVDVDSSGKK CKEKMISVLF ETKVQTEHKR FLAFEVKEYS
     ALDELQKEFE TAGLKKLFSE FVLALVK