ID   RWDD3_XENTR             Reviewed;         265 AA.
AC   A9ULH0; Q28D15;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-JUN-2008, sequence version 2.
DT   03-AUG-2022, entry version 60.
DE   RecName: Full=RWD domain-containing protein 3;
GN   Name=rwdd3; ORFNames=TGas086h03.1;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Egg;
RG   Sanger Xenopus tropicalis EST/cDNA project;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=N6; TISSUE=Ovary;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Enhancer of SUMO conjugation. Increases SUMO conjugation to
CC       proteins by promoting the: binding of E1 and E2 enzymes, thioester
CC       linkage between SUMO and ube2i/ubc9 and transfer of SUMO to specific
CC       target proteins which include hif1a, pias, nfkbia, nr3c1 and top1. Has
CC       no effect on ubiquitination (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Colocalizes with
CC       ubc9/ube2i in nuclear spots. {ECO:0000250}.
CC   -!- DOMAIN: The RWD domain is required for the sumoylation enhancement
CC       activity. {ECO:0000250}.
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DR   EMBL; CR855762; CAJ81611.1; -; mRNA.
DR   EMBL; BC157252; AAI57253.1; -; mRNA.
DR   RefSeq; NP_001017275.1; NM_001017275.2.
DR   AlphaFoldDB; A9ULH0; -.
DR   SMR; A9ULH0; -.
DR   STRING; 8364.ENSXETP00000054859; -.
DR   PaxDb; A9ULH0; -.
DR   PRIDE; A9ULH0; -.
DR   GeneID; 550029; -.
DR   KEGG; xtr:550029; -.
DR   CTD; 25950; -.
DR   Xenbase; XB-GENE-960546; rwdd3.
DR   eggNOG; ENOG502QSYH; Eukaryota.
DR   HOGENOM; CLU_087636_0_0_1; -.
DR   InParanoid; A9ULH0; -.
DR   OrthoDB; 1437243at2759; -.
DR   PhylomeDB; A9ULH0; -.
DR   TreeFam; TF324344; -.
DR   Reactome; R-XTR-3065678; SUMO is transferred from E1 to E2 (UBE2I, UBC9).
DR   Proteomes; UP000008143; Chromosome 4.
DR   Proteomes; UP000790000; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0032088; P:negative regulation of NF-kappaB transcription factor activity; IEA:InterPro.
DR   GO; GO:1902073; P:positive regulation of hypoxia-inducible factor-1alpha signaling pathway; IEA:InterPro.
DR   GO; GO:0033235; P:positive regulation of protein sumoylation; IBA:GO_Central.
DR   Gene3D; 3.10.110.10; -; 1.
DR   InterPro; IPR006575; RWD-domain.
DR   InterPro; IPR038840; RWDD3.
DR   InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR   PANTHER; PTHR15628; PTHR15628; 1.
DR   Pfam; PF05773; RWD; 1.
DR   SMART; SM00591; RWD; 1.
DR   SUPFAM; SSF54495; SSF54495; 1.
DR   PROSITE; PS50908; RWD; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Nucleus; Reference proteome.
FT   CHAIN           1..265
FT                   /note="RWD domain-containing protein 3"
FT                   /id="PRO_0000339465"
FT   DOMAIN          7..114
FT                   /note="RWD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00179"
FT   CONFLICT        19
FT                   /note="D -> V (in Ref. 2; AAI57253)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   265 AA;  30038 MW;  8BFFB06208D5D1C7 CRC64;
     MSESALEEVA ALSAIYCRDG ECEVLSSSVN GITVMIQTGV QGITGSEIHL KLIFDLPVEY
     PSSLPNISVS SEELTRAQRK DLRDKLVEQA KQHILEPMIH DLVVWTQQNL NNLIVQPEPS
     ILEGHFPLSL DTITEETTWT ILLRLDHMRA KSKYVKTVEK WTNDLKLCGR LMFLGKLILI
     LLQGDRKSTR DYLVLQKTCK VDVDSSGKKC KEKMISVLCE TILPLKQKRF TTFEVKEYSS
     VSDLQKEFEA AGLQTIFSEF VQALF