RWDD4_HUMAN
ID RWDD4_HUMAN Reviewed; 188 AA.
AC Q6NW29; B2RDE9; B4DDP2; Q75LA9; Q8WVW2;
DT 24-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 3.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=RWD domain-containing protein 4;
DE AltName: Full=Protein FAM28A;
GN Name=RWDD4; Synonyms=FAM28A, RWDD4A;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Tongue;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT LEU-124.
RC TISSUE=Testis, and Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full phosphorylation
RT site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [6]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- INTERACTION:
CC Q6NW29; P07686: HEXB; NbExp=3; IntAct=EBI-743971, EBI-7133736;
CC Q6NW29; Q9Y3S2: ZNF330; NbExp=6; IntAct=EBI-743971, EBI-373456;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6NW29-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6NW29-2; Sequence=VSP_056650;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAQ96848.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK293274; BAG56803.1; -; mRNA.
DR EMBL; AK315515; BAG37896.1; -; mRNA.
DR EMBL; AC093081; AAQ96848.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BC017472; AAH17472.1; -; mRNA.
DR EMBL; BC067752; AAH67752.1; -; mRNA.
DR EMBL; BC107432; AAI07433.1; -; mRNA.
DR CCDS; CCDS34111.1; -. [Q6NW29-1]
DR RefSeq; NP_001294851.1; NM_001307922.1.
DR RefSeq; NP_689895.2; NM_152682.3. [Q6NW29-1]
DR AlphaFoldDB; Q6NW29; -.
DR SMR; Q6NW29; -.
DR BioGRID; 128409; 19.
DR IntAct; Q6NW29; 6.
DR STRING; 9606.ENSP00000388920; -.
DR iPTMnet; Q6NW29; -.
DR PhosphoSitePlus; Q6NW29; -.
DR BioMuta; RWDD4; -.
DR DMDM; 116242778; -.
DR EPD; Q6NW29; -.
DR jPOST; Q6NW29; -.
DR MassIVE; Q6NW29; -.
DR MaxQB; Q6NW29; -.
DR PaxDb; Q6NW29; -.
DR PeptideAtlas; Q6NW29; -.
DR PRIDE; Q6NW29; -.
DR ProteomicsDB; 3876; -.
DR ProteomicsDB; 66737; -. [Q6NW29-1]
DR Antibodypedia; 45760; 44 antibodies from 10 providers.
DR DNASU; 201965; -.
DR Ensembl; ENST00000326397.10; ENSP00000388920.2; ENSG00000182552.15. [Q6NW29-1]
DR Ensembl; ENST00000510968.5; ENSP00000426329.1; ENSG00000182552.15. [Q6NW29-2]
DR GeneID; 201965; -.
DR KEGG; hsa:201965; -.
DR MANE-Select; ENST00000326397.10; ENSP00000388920.2; NM_152682.4; NP_689895.2.
DR UCSC; uc063bgm.1; human. [Q6NW29-1]
DR CTD; 201965; -.
DR DisGeNET; 201965; -.
DR GeneCards; RWDD4; -.
DR HGNC; HGNC:23750; RWDD4.
DR HPA; ENSG00000182552; Low tissue specificity.
DR neXtProt; NX_Q6NW29; -.
DR OpenTargets; ENSG00000182552; -.
DR PharmGKB; PA142670965; -.
DR VEuPathDB; HostDB:ENSG00000182552; -.
DR eggNOG; KOG4018; Eukaryota.
DR GeneTree; ENSGT00390000015545; -.
DR HOGENOM; CLU_123949_0_0_1; -.
DR InParanoid; Q6NW29; -.
DR OMA; LGCGMTY; -.
DR OrthoDB; 1549838at2759; -.
DR PhylomeDB; Q6NW29; -.
DR TreeFam; TF326409; -.
DR PathwayCommons; Q6NW29; -.
DR SignaLink; Q6NW29; -.
DR BioGRID-ORCS; 201965; 104 hits in 1042 CRISPR screens.
DR GenomeRNAi; 201965; -.
DR Pharos; Q6NW29; Tdark.
DR PRO; PR:Q6NW29; -.
DR Proteomes; UP000005640; Chromosome 4.
DR RNAct; Q6NW29; protein.
DR Bgee; ENSG00000182552; Expressed in secondary oocyte and 189 other tissues.
DR ExpressionAtlas; Q6NW29; baseline and differential.
DR Genevisible; Q6NW29; HS.
DR Gene3D; 3.10.110.10; -; 1.
DR InterPro; IPR006575; RWD-domain.
DR InterPro; IPR042770; RWDD4.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR PANTHER; PTHR21275; PTHR21275; 1.
DR Pfam; PF05773; RWD; 1.
DR SMART; SM00591; RWD; 1.
DR SUPFAM; SSF54495; SSF54495; 1.
DR PROSITE; PS50908; RWD; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22814378"
FT CHAIN 2..188
FT /note="RWD domain-containing protein 4"
FT /id="PRO_0000076307"
FT DOMAIN 9..111
FT /note="RWD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00179"
FT REGION 132..167
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 137..167
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT VAR_SEQ 1..95
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_056650"
FT VARIANT 124
FT /note="I -> L (in dbSNP:rs10015804)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_024928"
FT CONFLICT 19
FT /note="E -> G (in Ref. 2)"
FT /evidence="ECO:0000305"
FT CONFLICT 34
FT /note="Y -> H (in Ref. 2)"
FT /evidence="ECO:0000305"
FT CONFLICT 88
FT /note="V -> A (in Ref. 2; AAQ96848)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 188 AA; 21251 MW; 2A100A29E526F8D6 CRC64;
MSANEDQEME LEALRSIYEG DESFRELSPV SFQYRIGENG DPKAFLIEIS WTETYPQTPP
ILSMNAFFNN TISSAVKQSI LAKLQEAVEA NLGTAMTYTL FEYAKDNKEQ FMENHNPINS
ATSISNIISI ETPNTAPSSK KKDKKEQLSK AQKRKLADKT DHKGELPRGW NWVDVVKHLS
KTGSKDDE
