ID   RX2_ORYLA               Reviewed;         327 AA.
AC   Q9I9A2; Q9YHA2;
DT   26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 122.
DE   RecName: Full=Retinal homeobox protein Rx2;
GN   Name=rx2;
OS   Oryzias latipes (Japanese rice fish) (Japanese killifish).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Ovalentaria; Atherinomorphae; Beloniformes; Adrianichthyidae; Oryziinae;
OC   Oryzias.
OX   NCBI_TaxID=8090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=10751179; DOI=10.1242/dev.127.9.1911;
RA   Winkler S., Loosli F., Henrich T., Wakamatsu Y., Wittbrodt J.;
RT   "The conditional medaka mutation eyeless uncouples patterning and
RT   morphogenesis of the eye.";
RL   Development 127:1911-1919(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 128-315.
RX   PubMed=10090721; DOI=10.1101/gad.13.6.649;
RA   Loosli F., Winkler S., Wittbrodt J.;
RT   "Six3 overexpression initiates the formation of ectopic retina.";
RL   Genes Dev. 13:649-654(1999).
CC   -!- FUNCTION: Plays a critical role in eye formation by regulating the
CC       initial specification of retinal cells and/or their subsequent
CC       proliferation. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC       ECO:0000255|PROSITE-ProRule:PRU00138}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in the retina from early somitogenesis
CC       (stage 19) onwards.
CC   -!- SIMILARITY: Belongs to the paired homeobox family. Bicoid subfamily.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AJ250405; CAB88703.1; -; mRNA.
DR   EMBL; AJ007939; CAA07775.1; -; mRNA.
DR   RefSeq; NP_001098373.1; NM_001104903.1.
DR   AlphaFoldDB; Q9I9A2; -.
DR   SMR; Q9I9A2; -.
DR   STRING; 8090.ENSORLP00000022547; -.
DR   Ensembl; ENSORLT00000022548; ENSORLP00000022547; ENSORLG00000018007.
DR   GeneID; 100125458; -.
DR   KEGG; ola:100125458; -.
DR   CTD; 30473; -.
DR   eggNOG; KOG0490; Eukaryota.
DR   GeneTree; ENSGT00940000163917; -.
DR   HOGENOM; CLU_047013_2_0_1; -.
DR   InParanoid; Q9I9A2; -.
DR   OrthoDB; 1085093at2759; -.
DR   Proteomes; UP000001038; Chromosome 17.
DR   Proteomes; UP000265180; Unplaced.
DR   Proteomes; UP000265200; Unplaced.
DR   Bgee; ENSORLG00000018007; Expressed in sexually immature organism and 4 other tissues.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd00086; homeodomain; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR003654; OAR_dom.
DR   InterPro; IPR043562; RAX/RAX2.
DR   PANTHER; PTHR46271; PTHR46271; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   Pfam; PF03826; OAR; 1.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   PROSITE; PS50803; OAR; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..327
FT                   /note="Retinal homeobox protein Rx2"
FT                   /id="PRO_0000049286"
FT   DNA_BIND        137..196
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          52..140
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           37..44
FT                   /note="Octapeptide motif"
FT   MOTIF           304..317
FT                   /note="OAR"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00138"
FT   MOTIF           310..314
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        99..133
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        128..133
FT                   /note="KEEEHG -> DDEQQP (in Ref. 2; CAA07775)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        226
FT                   /note="A -> G (in Ref. 2; CAA07775)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   327 AA;  36596 MW;  07F67AE710AAC443 CRC64;
     MHLSMDTLGM VDDSGHGIVD SYDMAKGAGS SNGGRVHSID VILGFTKDQE PLLHSVGDDG
     SPKVNGALQQ DPAKPVPSEP YGNLPELGDS SQHHSYHDSS LFSSDKCEEM SNLTKEVDIA
     DGSPKSIKEE EHGKKKHRRN RTTFTTYQLH ELERAFEKSH YPDVYSREEL ATKVNLPEVR
     VQVWFQNRRA KWRRQEKMDT STMKLHDSPM LSFNRPPMPP SVGQVANPMP LDPWLTSPIS
     SATPMHTIPG FMSSPQTLQP TYSSHSFLNS PPGMVQGMQP MGPPAYQCAP PFNDKYPVED
     DRNSSIAALR MKAKEHIQSM DKTWQHM