RXL7_BACSU
ID RXL7_BACSU Reviewed; 82 AA.
AC P46350;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 26-JUL-2002, sequence version 3.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Ribosome-associated protein L7Ae-like;
GN Name=rplGB; Synonyms=ybaB, ybxF; OrderedLocusNames=BSU01090;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC 3610 / NRRL NRS-744 / VKM B-501;
RX PubMed=7657605; DOI=10.1074/jbc.270.35.20329;
RA Boor K.J., Duncan M.L., Price C.W.;
RT "Genetic and transcriptional organization of the region encoding the beta
RT subunit of Bacillus subtilis RNA polymerase.";
RL J. Biol. Chem. 270:20329-20336(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=8969501; DOI=10.1099/13500872-142-11-3039;
RA Yasumoto K., Liu H., Jeong S.M., Ohashi Y., Kakinuma S., Tanaka K.,
RA Kawamura F., Yoshikawa H., Takahashi H.;
RT "Sequence analysis of a 50 kb region between spo0H and rrnH on the Bacillus
RT subtilis chromosome.";
RL Microbiology 142:3039-3046(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP RIBOSOME ASSOCIATION, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF LYS-17;
RP LYS-21 AND LYS-24.
RC STRAIN=168;
RX PubMed=17468242; DOI=10.1128/jb.01786-06;
RA Sojka L., Fucik V., Krasny L., Barvik I., Jonak J.;
RT "YbxF, a protein associated with exponential-phase ribosomes in Bacillus
RT subtilis.";
RL J. Bacteriol. 189:4809-4814(2007).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEX WITH RNA, FUNCTION, AND
RP RNA-BINDING.
RX PubMed=22355167; DOI=10.1261/rna.031518.111;
RA Baird N.J., Zhang J., Hamma T., Ferre-D'Amare A.R.;
RT "YbxF and YlxQ are bacterial homologs of L7Ae and bind K-turns but not K-
RT loops.";
RL RNA 18:759-770(2012).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) IN COMPLEX WITH K-TURN RNA.
RX PubMed=23892783; DOI=10.1038/nature12440;
RA Zhang J., Ferre-D'Amare A.R.;
RT "Co-crystal structure of a T-box riboswitch stem I domain in complex with
RT its cognate tRNA.";
RL Nature 500:363-366(2013).
CC -!- FUNCTION: RNA-binding protein that recognizes the K-turn motif present
CC in ribosomal RNA, but also in box C/D and box C'/D' sRNAs.
CC {ECO:0000269|PubMed:22355167}.
CC -!- SUBUNIT: Found associated with the 50S subunit of the ribosome during
CC exponential growth but not during stationary phase.
CC {ECO:0000269|PubMed:22355167, ECO:0000269|PubMed:23892783}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Note=Localizes to the cell pole in
CC exponential growth.
CC -!- DISRUPTION PHENOTYPE: None; has no effect on viability, growth or
CC sporulation at low, normal or elevated temperatures.
CC {ECO:0000269|PubMed:17468242}.
CC -!- SIMILARITY: Belongs to the eukaryotic ribosomal protein eL8 family.
CC {ECO:0000255|HAMAP-Rule:MF_00574}.
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DR EMBL; L43593; AAB59113.1; -; Genomic_DNA.
DR EMBL; D64127; BAA11000.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB11885.1; -; Genomic_DNA.
DR PIR; G69751; G69751.
DR RefSeq; NP_387990.1; NC_000964.3.
DR RefSeq; WP_003225778.1; NZ_JNCM01000029.1.
DR PDB; 3V7E; X-ray; 2.80 A; A/B=2-82.
DR PDB; 4LCK; X-ray; 3.20 A; A/D=2-82.
DR PDB; 4TZP; X-ray; 8.50 A; A/D=2-82.
DR PDB; 4TZV; X-ray; 5.03 A; A=2-82.
DR PDB; 4TZW; X-ray; 4.67 A; A=2-82.
DR PDB; 4TZZ; X-ray; 3.64 A; A/D/G/J=2-82.
DR PDBsum; 3V7E; -.
DR PDBsum; 4LCK; -.
DR PDBsum; 4TZP; -.
DR PDBsum; 4TZV; -.
DR PDBsum; 4TZW; -.
DR PDBsum; 4TZZ; -.
DR AlphaFoldDB; P46350; -.
DR SMR; P46350; -.
DR STRING; 224308.BSU01090; -.
DR PaxDb; P46350; -.
DR PRIDE; P46350; -.
DR EnsemblBacteria; CAB11885; CAB11885; BSU_01090.
DR GeneID; 64301947; -.
DR GeneID; 936829; -.
DR KEGG; bsu:BSU01090; -.
DR PATRIC; fig|224308.179.peg.112; -.
DR eggNOG; COG1358; Bacteria.
DR InParanoid; P46350; -.
DR OMA; KQTIRMV; -.
DR BioCyc; BSUB:BSU01090-MON; -.
DR PRO; PR:P46350; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005840; C:ribosome; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR GO; GO:0006412; P:translation; IEA:InterPro.
DR Gene3D; 3.30.1330.30; -; 1.
DR HAMAP; MF_00574; Ribosomal_L7Ae_Bact; 1.
DR InterPro; IPR029064; L30e-like.
DR InterPro; IPR004038; Ribosomal_L7Ae/L30e/S12e/Gad45.
DR InterPro; IPR000948; Ribosomal_L7Ae_prok.
DR InterPro; IPR023460; Ribosomal_L7Ae_putative.
DR Pfam; PF01248; Ribosomal_L7Ae; 1.
DR PRINTS; PR00884; RIBOSOMALHS6.
DR SUPFAM; SSF55315; SSF55315; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Reference proteome; Ribonucleoprotein;
KW Ribosomal protein; RNA-binding.
FT CHAIN 1..82
FT /note="Ribosome-associated protein L7Ae-like"
FT /id="PRO_0000136812"
FT MUTAGEN 17
FT /note="K->A: No effect."
FT /evidence="ECO:0000269|PubMed:17468242"
FT MUTAGEN 21
FT /note="K->A: No effect."
FT /evidence="ECO:0000269|PubMed:17468242"
FT MUTAGEN 24
FT /note="K->A: Decreased ribosomal association."
FT /evidence="ECO:0000269|PubMed:17468242"
FT HELIX 3..8
FT /evidence="ECO:0007829|PDB:3V7E"
FT STRAND 10..15
FT /evidence="ECO:0007829|PDB:3V7E"
FT HELIX 16..23
FT /evidence="ECO:0007829|PDB:3V7E"
FT TURN 24..26
FT /evidence="ECO:0007829|PDB:3V7E"
FT STRAND 28..34
FT /evidence="ECO:0007829|PDB:3V7E"
FT HELIX 39..52
FT /evidence="ECO:0007829|PDB:3V7E"
FT STRAND 56..60
FT /evidence="ECO:0007829|PDB:3V7E"
FT HELIX 62..68
FT /evidence="ECO:0007829|PDB:3V7E"
FT STRAND 76..81
FT /evidence="ECO:0007829|PDB:3V7E"
SQ SEQUENCE 82 AA; 8462 MW; B8E301C141E77211 CRC64;
MSYDKVSQAK SIIIGTKQTV KALKRGSVKE VVVAKDADPI LTSSVVSLAE DQGISVSMVE
SMKKLGKACG IEVGAAAVAI IL
