RXLAC_PHYIT
ID RXLAC_PHYIT Reviewed; 198 AA.
AC D0NFU7;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2009, sequence version 1.
DT 03-AUG-2022, entry version 31.
DE RecName: Full=Secreted RxLR effector protein PITG_22926 {ECO:0000303|PubMed:19741609};
DE Flags: Precursor;
GN ORFNames=PITG_22926;
OS Phytophthora infestans (strain T30-4) (Potato late blight agent).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=403677;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], DOMAIN, AND INDUCTION.
RC STRAIN=T30-4;
RX PubMed=19741609; DOI=10.1038/nature08358;
RG The Broad Institute Genome Sequencing Platform;
RA Haas B.J., Kamoun S., Zody M.C., Jiang R.H., Handsaker R.E., Cano L.M.,
RA Grabherr M., Kodira C.D., Raffaele S., Torto-Alalibo T., Bozkurt T.O.,
RA Ah-Fong A.M., Alvarado L., Anderson V.L., Armstrong M.R., Avrova A.,
RA Baxter L., Beynon J., Boevink P.C., Bollmann S.R., Bos J.I., Bulone V.,
RA Cai G., Cakir C., Carrington J.C., Chawner M., Conti L., Costanzo S.,
RA Ewan R., Fahlgren N., Fischbach M.A., Fugelstad J., Gilroy E.M., Gnerre S.,
RA Green P.J., Grenville-Briggs L.J., Griffith J., Grunwald N.J., Horn K.,
RA Horner N.R., Hu C.H., Huitema E., Jeong D.H., Jones A.M., Jones J.D.,
RA Jones R.W., Karlsson E.K., Kunjeti S.G., Lamour K., Liu Z., Ma L.,
RA Maclean D., Chibucos M.C., McDonald H., McWalters J., Meijer H.J.,
RA Morgan W., Morris P.F., Munro C.A., O'Neill K., Ospina-Giraldo M.,
RA Pinzon A., Pritchard L., Ramsahoye B., Ren Q., Restrepo S., Roy S.,
RA Sadanandom A., Savidor A., Schornack S., Schwartz D.C., Schumann U.D.,
RA Schwessinger B., Seyer L., Sharpe T., Silvar C., Song J., Studholme D.J.,
RA Sykes S., Thines M., van de Vondervoort P.J., Phuntumart V., Wawra S.,
RA Weide R., Win J., Young C., Zhou S., Fry W., Meyers B.C., van West P.,
RA Ristaino J., Govers F., Birch P.R., Whisson S.C., Judelson H.S.,
RA Nusbaum C.;
RT "Genome sequence and analysis of the Irish potato famine pathogen
RT Phytophthora infestans.";
RL Nature 461:393-398(2009).
RN [2]
RP DOMAIN, AND SUBCELLULAR LOCATION.
RX PubMed=30154258; DOI=10.1128/mbio.01216-18;
RA Wang S., Welsh L., Thorpe P., Whisson S.C., Boevink P.C., Birch P.R.J.;
RT "The Phytophthora infestans haustorium is a site for secretion of viverse
RT classes of infection-associated proteins.";
RL MBio 9:0-0(2018).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=30329083; DOI=10.1093/jxb/ery360;
RA Wang S., McLellan H., Bukharova T., He Q., Murphy F., Shi J., Sun S.,
RA van Weymers P., Ren Y., Thilliez G., Wang H., Chen X., Engelhardt S.,
RA Vleeshouwers V., Gilroy E.M., Whisson S.C., Hein I., Wang X., Tian Z.,
RA Birch P.R.J., Boevink P.C.;
RT "Phytophthora infestans RXLR effectors act in concert at diverse
RT subcellular locations to enhance host colonization.";
RL J. Exp. Bot. 70:343-356(2019).
RN [4]
RP FUNCTION, INTERACTION WITH HOST MAPKBETA2, AND SUBCELLULAR LOCATION.
RX PubMed=31217198; DOI=10.1104/pp.18.00625;
RA Ren Y., Armstrong M., Qi Y., McLellan H., Zhong C., Du B., Birch P.R.J.,
RA Tian Z.;
RT "Phytophthora infestans RXLR effectors target parallel steps in an immune
RT signal transduction pathway.";
RL Plant Physiol. 180:2227-2239(2019).
CC -!- FUNCTION: Secreted effector that promotes P.infestans colonization of
CC plant host (PubMed:30329083, PubMed:31217198). Specifically suppresses
CC Avr4/Cf4- and AvrPto/Pto-triggered cell death (PubMed:31217198).
CC Targets the potato MAP3Kbeta2 kinase, a positive regulator of cell
CC death associated with plant immunity, and perturbs signaling pathways
CC triggered by MAP3Kbeta2 (PubMed:31217198).
CC {ECO:0000269|PubMed:30329083, ECO:0000269|PubMed:31217198}.
CC -!- SUBUNIT: Interacts with host MAP3Kbeta2 in the nucleoplasm.
CC {ECO:0000269|PubMed:31217198}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:30154258,
CC ECO:0000269|PubMed:30329083, ECO:0000269|PubMed:31217198}. Host nucleus
CC {ECO:0000269|PubMed:30154258, ECO:0000269|PubMed:30329083,
CC ECO:0000269|PubMed:31217198}. Host nucleus, host nucleolus
CC {ECO:0000269|PubMed:30154258, ECO:0000269|PubMed:30329083}. Note=Is
CC secreted from haustoria to be delivered into host cells.
CC {ECO:0000269|PubMed:30154258, ECO:0000269|PubMed:30329083}.
CC -!- INDUCTION: Expression is up-regulated during the early plant infection
CC stages. {ECO:0000269|PubMed:19741609}.
CC -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC phosphate. {ECO:0000305|PubMed:19741609}.
CC -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR EMBL; DS028136; EEY57148.1; -; Genomic_DNA.
DR RefSeq; XP_002901758.1; XM_002901712.1.
DR AlphaFoldDB; D0NFU7; -.
DR SMR; D0NFU7; -.
DR EnsemblProtists; PITG_22926T0; PITG_22926T0; PITG_22926.
DR GeneID; 9476146; -.
DR KEGG; pif:PITG_22926; -.
DR VEuPathDB; FungiDB:PITG_22926; -.
DR eggNOG; ENOG502RGQG; Eukaryota.
DR HOGENOM; CLU_122622_0_0_1; -.
DR InParanoid; D0NFU7; -.
DR OMA; HDSAQSK; -.
DR OrthoDB; 1628730at2759; -.
DR Proteomes; UP000006643; Partially assembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0044196; C:host cell nucleolus; IEA:UniProtKB-SubCell.
PE 1: Evidence at protein level;
KW Host nucleus; Reference proteome; Secreted; Signal; Virulence.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..198
FT /note="Secreted RxLR effector protein PITG_22926"
FT /id="PRO_5003013353"
FT MOTIF 43..52
FT /note="RxLR-dEER"
FT /evidence="ECO:0000305|PubMed:19741609"
SQ SEQUENCE 198 AA; 22462 MW; AEC815E738B4C548 CRC64;
MLRSFLLIVA TVSLFGQCKP LPLATSPVSD AVRAPHRSTH ETRFLRTNDE ERGATMTLAG
VLRDKAQTKQ LLTSWLNSGK SVPSVSNKLG LKRMSLEQAI HHENWKALTT FQRMKSKKAK
AYAKYGTGYQ TEAKTKENLL QWVMRGDSPK EVSSTLGLLG LSRRKIIDHQ NYEAFRTFLK
YRKQWAEMQG NGFTKLTT
