RXLRA_PHYIT
ID RXLRA_PHYIT Reviewed; 170 AA.
AC D0N4E0;
DT 08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2009, sequence version 1.
DT 03-AUG-2022, entry version 33.
DE RecName: Full=RxLR effector protein PITG_22798 {ECO:0000303|PubMed:30329083};
DE Flags: Precursor;
GN ORFNames=PITG_22798;
OS Phytophthora infestans (strain T30-4) (Potato late blight agent).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=403677;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND INDUCTION.
RC STRAIN=T30-4;
RX PubMed=19741609; DOI=10.1038/nature08358;
RG The Broad Institute Genome Sequencing Platform;
RA Haas B.J., Kamoun S., Zody M.C., Jiang R.H., Handsaker R.E., Cano L.M.,
RA Grabherr M., Kodira C.D., Raffaele S., Torto-Alalibo T., Bozkurt T.O.,
RA Ah-Fong A.M., Alvarado L., Anderson V.L., Armstrong M.R., Avrova A.,
RA Baxter L., Beynon J., Boevink P.C., Bollmann S.R., Bos J.I., Bulone V.,
RA Cai G., Cakir C., Carrington J.C., Chawner M., Conti L., Costanzo S.,
RA Ewan R., Fahlgren N., Fischbach M.A., Fugelstad J., Gilroy E.M., Gnerre S.,
RA Green P.J., Grenville-Briggs L.J., Griffith J., Grunwald N.J., Horn K.,
RA Horner N.R., Hu C.H., Huitema E., Jeong D.H., Jones A.M., Jones J.D.,
RA Jones R.W., Karlsson E.K., Kunjeti S.G., Lamour K., Liu Z., Ma L.,
RA Maclean D., Chibucos M.C., McDonald H., McWalters J., Meijer H.J.,
RA Morgan W., Morris P.F., Munro C.A., O'Neill K., Ospina-Giraldo M.,
RA Pinzon A., Pritchard L., Ramsahoye B., Ren Q., Restrepo S., Roy S.,
RA Sadanandom A., Savidor A., Schornack S., Schwartz D.C., Schumann U.D.,
RA Schwessinger B., Seyer L., Sharpe T., Silvar C., Song J., Studholme D.J.,
RA Sykes S., Thines M., van de Vondervoort P.J., Phuntumart V., Wawra S.,
RA Weide R., Win J., Young C., Zhou S., Fry W., Meyers B.C., van West P.,
RA Ristaino J., Govers F., Birch P.R., Whisson S.C., Judelson H.S.,
RA Nusbaum C.;
RT "Genome sequence and analysis of the Irish potato famine pathogen
RT Phytophthora infestans.";
RL Nature 461:393-398(2009).
RN [2]
RP INDUCTION, AND DOMAIN.
RX PubMed=29312401; DOI=10.3389/fpls.2017.02155;
RA Yin J., Gu B., Huang G., Tian Y., Quan J., Lindqvist-Kreuze H., Shan W.;
RT "Conserved RXLR effector genes of Phytophthora infestans expressed at the
RT early stage of potato infection are suppressive to host defense.";
RL Front. Plant Sci. 8:2155-2155(2017).
RN [3]
RP INDUCTION, FUNCTION, SUBCELLULAR LOCATION, DOMAIN, AND MUTAGENESIS OF
RP 40-ARG--ARG-47; 157-LYS--ARG-167 AND 165-ARG--ARG-167.
RX PubMed=28216607; DOI=10.3390/ijms18020409;
RA Wang H., Ren Y., Zhou J., Du J., Hou J., Jiang R., Wang H., Tian Z.,
RA Xie C.;
RT "The cell death triggered by the nuclear localized RxLR effector PITG_22798
RT from Phytophthora infestans is suppressed by the effector Avr3b.";
RL Int. J. Mol. Sci. 18:0-0(2017).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=30329083; DOI=10.1093/jxb/ery360;
RA Wang S., McLellan H., Bukharova T., He Q., Murphy F., Shi J., Sun S.,
RA van Weymers P., Ren Y., Thilliez G., Wang H., Chen X., Engelhardt S.,
RA Vleeshouwers V., Gilroy E.M., Whisson S.C., Hein I., Wang X., Tian Z.,
RA Birch P.R.J., Boevink P.C.;
RT "Phytophthora infestans RXLR effectors act in concert at diverse
RT subcellular locations to enhance host colonization.";
RL J. Exp. Bot. 70:343-356(2019).
CC -!- FUNCTION: Effector that enhances P.infestans colonization of Nicotiana
CC benthamiana leaves, which suggests that PITG_22798 contributes to
CC P.infestans infection (PubMed:28216607). Triggers cell death in
CC Nicotiana benthamiana. PITG_22798-triggered cell death is dependent on
CC SGT1-mediated signaling but not HSP90, and is suppressed by the
CC P.infestans avirulence effector 3b (AVR3b) (PubMed:28216607).
CC {ECO:0000269|PubMed:28216607}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:28216607,
CC ECO:0000269|PubMed:30329083}. Host nucleus
CC {ECO:0000269|PubMed:28216607, ECO:0000269|PubMed:30329083}.
CC Note=Lovalization to host nucleus is require to induce cell death.
CC {ECO:0000269|PubMed:28216607}.
CC -!- INDUCTION: Expression is induced early during infection of P.infestans.
CC {ECO:0000269|PubMed:19741609, ECO:0000269|PubMed:28216607,
CC ECO:0000269|PubMed:29312401}.
CC -!- DOMAIN: The nuclear localization signal is required for the
CC localization into the host nucleus and the ability to induce host cell
CC death. {ECO:0000269|PubMed:28216607}.
CC -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC phosphate. {ECO:0000269|PubMed:28216607, ECO:0000305|PubMed:29312401}.
CC -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR EMBL; DS028125; EEY69748.1; -; Genomic_DNA.
DR RefSeq; XP_002998395.1; XM_002998349.1.
DR AlphaFoldDB; D0N4E0; -.
DR EnsemblProtists; PITG_22798T0; PITG_22798T0; PITG_22798.
DR GeneID; 9465932; -.
DR KEGG; pif:PITG_22798; -.
DR VEuPathDB; FungiDB:PITG_22798; -.
DR eggNOG; ENOG502RGVV; Eukaryota.
DR HOGENOM; CLU_1499162_0_0_1; -.
DR OMA; VFERWHA; -.
DR OrthoDB; 809000at2759; -.
DR Proteomes; UP000006643; Partially assembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR InterPro; IPR031825; RXLR.
DR Pfam; PF16810; RXLR; 1.
PE 1: Evidence at protein level;
KW Host nucleus; Reference proteome; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..170
FT /note="RxLR effector protein PITG_22798"
FT /id="PRO_5003012931"
FT MOTIF 43..59
FT /note="RxLR-dEER"
FT /evidence="ECO:0000305|PubMed:29312401"
FT MOTIF 157..167
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000269|PubMed:28216607"
FT MUTAGEN 40..47
FT /note="Missing: Leads to the inability to induce host cell
FT death."
FT /evidence="ECO:0000269|PubMed:28216607"
FT MUTAGEN 157..167
FT /note="Missing: Leads to host cytoplasmic accumulation and
FT the inability to induce host cell death."
FT /evidence="ECO:0000269|PubMed:28216607"
FT MUTAGEN 165..167
FT /note="RKR->AAA: Leads to host cytoplasmic accumulation and
FT the inability to induce host cell death."
FT /evidence="ECO:0000269|PubMed:28216607"
SQ SEQUENCE 170 AA; 19226 MW; B6743FA5186F3DA4 CRC64;
MRCYYVLIAI AAVLSGIATA AADSRHNKLA AVDSDKTTDR VELFLRRNDE LDAENTDERI
VAGAIPLNAR IIKRLVKLEK KMVDPKLADD LLEGTTLKTE LDAALPYAGR TQVFERWHAQ
GLDPSSITKA VNIHPAIEKK YRIVYMMYEL FVKSADKKRL AKLKRKRTND
