RXLRC_PHYIT
ID RXLRC_PHYIT Reviewed; 135 AA.
AC D0MXE4;
DT 08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2009, sequence version 1.
DT 03-AUG-2022, entry version 33.
DE RecName: Full=RxLR effector protein PITG_02860 {ECO:0000303|PubMed:30049706};
DE Flags: Precursor;
GN ORFNames=PITG_02860;
OS Phytophthora infestans (strain T30-4) (Potato late blight agent).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=403677;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=T30-4;
RX PubMed=19741609; DOI=10.1038/nature08358;
RG The Broad Institute Genome Sequencing Platform;
RA Haas B.J., Kamoun S., Zody M.C., Jiang R.H., Handsaker R.E., Cano L.M.,
RA Grabherr M., Kodira C.D., Raffaele S., Torto-Alalibo T., Bozkurt T.O.,
RA Ah-Fong A.M., Alvarado L., Anderson V.L., Armstrong M.R., Avrova A.,
RA Baxter L., Beynon J., Boevink P.C., Bollmann S.R., Bos J.I., Bulone V.,
RA Cai G., Cakir C., Carrington J.C., Chawner M., Conti L., Costanzo S.,
RA Ewan R., Fahlgren N., Fischbach M.A., Fugelstad J., Gilroy E.M., Gnerre S.,
RA Green P.J., Grenville-Briggs L.J., Griffith J., Grunwald N.J., Horn K.,
RA Horner N.R., Hu C.H., Huitema E., Jeong D.H., Jones A.M., Jones J.D.,
RA Jones R.W., Karlsson E.K., Kunjeti S.G., Lamour K., Liu Z., Ma L.,
RA Maclean D., Chibucos M.C., McDonald H., McWalters J., Meijer H.J.,
RA Morgan W., Morris P.F., Munro C.A., O'Neill K., Ospina-Giraldo M.,
RA Pinzon A., Pritchard L., Ramsahoye B., Ren Q., Restrepo S., Roy S.,
RA Sadanandom A., Savidor A., Schornack S., Schwartz D.C., Schumann U.D.,
RA Schwessinger B., Seyer L., Sharpe T., Silvar C., Song J., Studholme D.J.,
RA Sykes S., Thines M., van de Vondervoort P.J., Phuntumart V., Wawra S.,
RA Weide R., Win J., Young C., Zhou S., Fry W., Meyers B.C., van West P.,
RA Ristaino J., Govers F., Birch P.R., Whisson S.C., Judelson H.S.,
RA Nusbaum C.;
RT "Genome sequence and analysis of the Irish potato famine pathogen
RT Phytophthora infestans.";
RL Nature 461:393-398(2009).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH HOST NRL1.
RX PubMed=26966171; DOI=10.1104/pp.16.00178;
RA Yang L., McLellan H., Naqvi S., He Q., Boevink P.C., Armstrong M.,
RA Giuliani L.M., Zhang W., Tian Z., Zhan J., Gilroy E.M., Birch P.R.;
RT "Potato NPH3/RPT2-Like Protein StNRL1, Targeted by a Phytophthora infestans
RT RXLR Effector, Is a Susceptibility Factor.";
RL Plant Physiol. 171:645-657(2016).
RN [3]
RP FUNCTION, INTERACTION WITH HOST NRL1, DOMAIN, AND MUTAGENESIS OF
RP 126-LEU--PRO-135.
RX PubMed=30049706; DOI=10.1073/pnas.1808585115;
RA He Q., Naqvi S., McLellan H., Boevink P.C., Champouret N., Hein I.,
RA Birch P.R.J.;
RT "Plant pathogen effector utilizes host susceptibility factor NRL1 to
RT degrade the immune regulator SWAP70.";
RL Proc. Natl. Acad. Sci. U.S.A. 115:E7834-E7843(2018).
CC -!- FUNCTION: Effector that promotes P.infestans virulence and suppresses
CC pattern-triggered immunity (PTI) (PubMed:26966171, PubMed:30049706).
CC Interacts with the host ubiquitin E3 ligase NRL1 and enhances the
CC association between NRL1 and SWAP70 to promote proteasome-mediated
CC degradation of SWAP70, which results in the suppression of immunity
CC (PubMed:26966171, PubMed:30049706). {ECO:0000269|PubMed:26966171,
CC ECO:0000269|PubMed:30049706}.
CC -!- SUBUNIT: Interacts with host ubiquitin E3 ligase NRL1.
CC {ECO:0000269|PubMed:30049706}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:26966171}. Host
CC cytoplasm {ECO:0000269|PubMed:26966171}. Host nucleus, host nucleoplasm
CC {ECO:0000269|PubMed:26966171}. Note=The cytoplasmic localization is
CC more important for its contribution to virulence than the nucleoplasmic
CC localization. {ECO:0000269|PubMed:26966171}.
CC -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC phosphate. {ECO:0000305|PubMed:26966171}.
CC -!- DOMAIN: The C-terminus (residues 126 to 135) is required for the
CC interaction with host NRL1 and the ability to enhance the association
CC of NRL1 with SWAP70. {ECO:0000269|PubMed:30049706}.
CC -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR EMBL; DS028120; EEY64307.1; -; Genomic_DNA.
DR RefSeq; XP_002907743.1; XM_002907697.1.
DR AlphaFoldDB; D0MXE4; -.
DR EnsemblProtists; PITG_02860T0; PITG_02860T0; PITG_02860.
DR GeneID; 9476955; -.
DR KEGG; pif:PITG_02860; -.
DR VEuPathDB; FungiDB:PITG_02860; -.
DR HOGENOM; CLU_160339_0_0_1; -.
DR InParanoid; D0MXE4; -.
DR Proteomes; UP000006643; Partially assembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0044095; C:host cell nucleoplasm; IEA:UniProtKB-SubCell.
PE 1: Evidence at protein level;
KW Host cytoplasm; Host nucleus; Reference proteome; Secreted; Signal;
KW Virulence.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..135
FT /note="RxLR effector protein PITG_02860"
FT /id="PRO_5003011501"
FT REGION 126..135
FT /note="NRL1-binding domain"
FT /evidence="ECO:0000305|PubMed:30049706"
FT MOTIF 48..64
FT /note="RxLR-dEER"
FT /evidence="ECO:0000305|PubMed:26966171"
FT MUTAGEN 126..135
FT /note="Missing: Impairs the interaction with host NRL1 and
FT blocks the ability to enhance the association of NRL1 with
FT SWAP70."
FT /evidence="ECO:0000269|PubMed:30049706"
SQ SEQUENCE 135 AA; 15236 MW; E7A7FC4CB81F412D CRC64;
MRLAFLLLAV SHFICGNALP TNVKSSPVVS PGLIQSFDNA QKPVVMSRKL LRTDERLSEA
NEERTKLSEL FRLDDDEVAA IKELSSMFSA FLQKKSQAFD VYDEIFRSGY PIDTAARISN
LYTKYLKDPQ AFRGP
