ID   RXLRI_PHYIT             Reviewed;         173 AA.
AC   D0NEV2;
DT   08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2009, sequence version 1.
DT   03-AUG-2022, entry version 31.
DE   RecName: Full=RxLR effector protein PITG_10232 {ECO:0000303|PubMed:30329083};
DE   Flags: Precursor;
GN   ORFNames=PITG_10232;
OS   Phytophthora infestans (strain T30-4) (Potato late blight agent).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Phytophthora.
OX   NCBI_TaxID=403677;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=T30-4;
RX   PubMed=19741609; DOI=10.1038/nature08358;
RG   The Broad Institute Genome Sequencing Platform;
RA   Haas B.J., Kamoun S., Zody M.C., Jiang R.H., Handsaker R.E., Cano L.M.,
RA   Grabherr M., Kodira C.D., Raffaele S., Torto-Alalibo T., Bozkurt T.O.,
RA   Ah-Fong A.M., Alvarado L., Anderson V.L., Armstrong M.R., Avrova A.,
RA   Baxter L., Beynon J., Boevink P.C., Bollmann S.R., Bos J.I., Bulone V.,
RA   Cai G., Cakir C., Carrington J.C., Chawner M., Conti L., Costanzo S.,
RA   Ewan R., Fahlgren N., Fischbach M.A., Fugelstad J., Gilroy E.M., Gnerre S.,
RA   Green P.J., Grenville-Briggs L.J., Griffith J., Grunwald N.J., Horn K.,
RA   Horner N.R., Hu C.H., Huitema E., Jeong D.H., Jones A.M., Jones J.D.,
RA   Jones R.W., Karlsson E.K., Kunjeti S.G., Lamour K., Liu Z., Ma L.,
RA   Maclean D., Chibucos M.C., McDonald H., McWalters J., Meijer H.J.,
RA   Morgan W., Morris P.F., Munro C.A., O'Neill K., Ospina-Giraldo M.,
RA   Pinzon A., Pritchard L., Ramsahoye B., Ren Q., Restrepo S., Roy S.,
RA   Sadanandom A., Savidor A., Schornack S., Schwartz D.C., Schumann U.D.,
RA   Schwessinger B., Seyer L., Sharpe T., Silvar C., Song J., Studholme D.J.,
RA   Sykes S., Thines M., van de Vondervoort P.J., Phuntumart V., Wawra S.,
RA   Weide R., Win J., Young C., Zhou S., Fry W., Meyers B.C., van West P.,
RA   Ristaino J., Govers F., Birch P.R., Whisson S.C., Judelson H.S.,
RA   Nusbaum C.;
RT   "Genome sequence and analysis of the Irish potato famine pathogen
RT   Phytophthora infestans.";
RL   Nature 461:393-398(2009).
RN   [2]
RP   INDUCTION.
RX   PubMed=28228125; DOI=10.1186/s12864-017-3585-x;
RA   Ah-Fong A.M., Kim K.S., Judelson H.S.;
RT   "RNA-seq of life stages of the oomycete Phytophthora infestans reveals
RT   dynamic changes in metabolic, signal transduction, and pathogenesis genes
RT   and a major role for calcium signaling in development.";
RL   BMC Genomics 18:198-198(2017).
RN   [3]
RP   INDUCTION, AND DOMAIN.
RX   PubMed=29312401; DOI=10.3389/fpls.2017.02155;
RA   Yin J., Gu B., Huang G., Tian Y., Quan J., Lindqvist-Kreuze H., Shan W.;
RT   "Conserved RXLR effector genes of Phytophthora infestans expressed at the
RT   early stage of potato infection are suppressive to host defense.";
RL   Front. Plant Sci. 8:2155-2155(2017).
RN   [4]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=30329083; DOI=10.1093/jxb/ery360;
RA   Wang S., McLellan H., Bukharova T., He Q., Murphy F., Shi J., Sun S.,
RA   van Weymers P., Ren Y., Thilliez G., Wang H., Chen X., Engelhardt S.,
RA   Vleeshouwers V., Gilroy E.M., Whisson S.C., Hein I., Wang X., Tian Z.,
RA   Birch P.R.J., Boevink P.C.;
RT   "Phytophthora infestans RXLR effectors act in concert at diverse
RT   subcellular locations to enhance host colonization.";
RL   J. Exp. Bot. 70:343-356(2019).
CC   -!- FUNCTION: Effector that leads to host programmed cell death.
CC       {ECO:0000269|PubMed:30329083}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:30329083}. Host
CC       nucleus {ECO:0000269|PubMed:30329083}. Host cytoplasm
CC       {ECO:0000269|PubMed:30329083}.
CC   -!- INDUCTION: Expression is induced during host plant infection.
CC       {ECO:0000269|PubMed:28228125, ECO:0000269|PubMed:29312401}.
CC   -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC       cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC       phosphate. {ECO:0000305|PubMed:29312401}.
CC   -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR   EMBL; DS028135; EEY56741.1; -; Genomic_DNA.
DR   RefSeq; XP_002902069.1; XM_002902023.1.
DR   AlphaFoldDB; D0NEV2; -.
DR   EnsemblProtists; PITG_10232T0; PITG_10232T0; PITG_10232.
DR   GeneID; 9473890; -.
DR   KEGG; pif:PITG_10232; -.
DR   VEuPathDB; FungiDB:PITG_10232; -.
DR   HOGENOM; CLU_1550549_0_0_1; -.
DR   InParanoid; D0NEV2; -.
DR   Proteomes; UP000006643; Partially assembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
PE   2: Evidence at transcript level;
KW   Host cytoplasm; Host nucleus; Reference proteome; Secreted; Signal;
KW   Virulence.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..173
FT                   /note="RxLR effector protein PITG_10232"
FT                   /id="PRO_5003013297"
FT   REGION          25..64
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           46..65
FT                   /note="RxLR-dEER"
FT                   /evidence="ECO:0000305|PubMed:29312401"
FT   COMPBIAS        33..63
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   173 AA;  19642 MW;  6EC4E7AE4AB09DD6 CRC64;
     MRLGYLIVGC AVALLATTDG VVDASSKHKQ LSTDVPRPAD DISSERFLRS QDTPEDDGNP
     AHEDRGYLTV LTNAAMHPLR ERKMKNQIAN LIKMDATDAE LYAAGVQPHR LFDVIRHKDE
     SVEMSLSQWV TMVHQHQFPS DYIWKSQAYR RFKQYAGFYD GMQRNGQRVA STT