BECNA_DICDI
ID BECNA_DICDI Reviewed; 1368 AA.
AC Q55CC5; Q86CS0;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 92.
DE RecName: Full=Beclin-1-like protein A;
DE AltName: Full=Autophagy-related protein 6A;
GN Name=atg6A; Synonyms=apg6, apg6A, atg6; ORFNames=DDB_G0269244;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=AX3 / DH1;
RX PubMed=12626495; DOI=10.1074/jbc.m212467200;
RA Otto G.P., Wu M.Y., Kazgan N., Anderson O.R., Kessin R.H.;
RT "Macroautophagy is required for multicellular development of the social
RT amoeba Dictyostelium discoideum.";
RL J. Biol. Chem. 278:17636-17645(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP FUNCTION.
RX PubMed=14736886; DOI=10.1074/jbc.m311139200;
RA Otto G.P., Wu M.Y., Kazgan N., Anderson O.R., Kessin R.H.;
RT "Dictyostelium macroautophagy mutants vary in the severity of their
RT developmental defects.";
RL J. Biol. Chem. 279:15621-15629(2004).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19667176; DOI=10.1073/pnas.0813319106;
RA Jia K., Thomas C., Akbar M., Sun Q., Adams-Huet B., Gilpin C., Levine B.;
RT "Autophagy genes protect against Salmonella typhimurium infection and
RT mediate insulin signaling-regulated pathogen resistance.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:14564-14569(2009).
CC -!- FUNCTION: Involved in autophagy (PubMed:12626495, PubMed:14736886). May
CC be required to recruit the atg8-phosphatidylinositol conjugate and the
CC atg12-atg5 conjugate to the pre-autophagosomal structure (By
CC similarity). Required for normal survival when exposed to pathogenic
CC bacteria S.typhimurium by promoting autophagic degradation of
CC intracellular S.typhimurium (PubMed:19667176).
CC {ECO:0000250|UniProtKB:Q02948, ECO:0000269|PubMed:12626495,
CC ECO:0000269|PubMed:14736886, ECO:0000269|PubMed:19667176}.
CC -!- SUBCELLULAR LOCATION: Endosome membrane {ECO:0000250}; Peripheral
CC membrane protein {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Impaired survival when exposed to pathogenic
CC bacteria S.typhimurium associated with the accumulation of
CC S.typhimuriumin in large vacuoles which fail to undergo autophagy.
CC {ECO:0000269|PubMed:19667176}.
CC -!- SIMILARITY: Belongs to the beclin family. {ECO:0000305}.
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DR EMBL; AY191013; AAO39076.1; -; Genomic_DNA.
DR EMBL; AAFI02000005; EAL71972.1; -; Genomic_DNA.
DR RefSeq; XP_646556.1; XM_641464.1.
DR AlphaFoldDB; Q55CC5; -.
DR SMR; Q55CC5; -.
DR STRING; 44689.DDB0191425; -.
DR PaxDb; Q55CC5; -.
DR EnsemblProtists; EAL71972; EAL71972; DDB_G0269244.
DR GeneID; 8617524; -.
DR KEGG; ddi:DDB_G0269244; -.
DR dictyBase; DDB_G0269244; atg6A.
DR eggNOG; KOG2751; Eukaryota.
DR HOGENOM; CLU_256528_0_0_1; -.
DR InParanoid; Q55CC5; -.
DR OMA; EINSAWG; -.
DR PRO; PR:Q55CC5; -.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000407; C:phagophore assembly site; IBA:GO_Central.
DR GO; GO:0034271; C:phosphatidylinositol 3-kinase complex, class III, type I; IBA:GO_Central.
DR GO; GO:0034272; C:phosphatidylinositol 3-kinase complex, class III, type II; IBA:GO_Central.
DR GO; GO:0000045; P:autophagosome assembly; IBA:GO_Central.
DR GO; GO:0006995; P:cellular response to nitrogen starvation; IMP:dictyBase.
DR GO; GO:0045324; P:late endosome to vacuole transport; IBA:GO_Central.
DR GO; GO:0016236; P:macroautophagy; IMP:dictyBase.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0001878; P:response to yeast; IMP:dictyBase.
DR GO; GO:0030587; P:sorocarp development; IMP:dictyBase.
DR GO; GO:0031288; P:sorocarp morphogenesis; IMP:dictyBase.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IMP:dictyBase.
DR Gene3D; 1.10.418.40; -; 1.
DR InterPro; IPR007243; Atg6/Beclin.
DR InterPro; IPR038274; Atg6/Beclin_C_sf.
DR InterPro; IPR040455; Atg6_BARA.
DR PANTHER; PTHR12768; PTHR12768; 4.
DR Pfam; PF04111; APG6; 1.
PE 3: Inferred from homology;
KW Autophagy; Coiled coil; Endosome; Membrane; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..1368
FT /note="Beclin-1-like protein A"
FT /id="PRO_0000327429"
FT REGION 24..64
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 122..249
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 331..431
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 819..874
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 992..1048
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 1047..1150
FT /evidence="ECO:0000255"
FT COMPBIAS 1016..1048
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 1359
FT /note="L -> F (in Ref. 1; AAO39076)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1368 AA; 155666 MW; 76B560D5AA0925A0 CRC64;
MKKSDSSSSL VFSGIQIEIN NNYGSGSGSN NNNNNNNNNN NINNNNNPNN NPNNNGPPLR
PSSEWVLLSS SQPTIATTTN LTTNAPLSDS MIMNYVGNDN LNTPITPSTS SPYLKRNNSN
LNSIIENSNN SSPSNAITRN NSFNMDPNNN NNNNNNNNNN NNNNNNNGEY MNSSIVFDNN
VNNNNNNPNN NPNNNVHSNS HNTNNSITHS GSITSSNSNN NNSNNNNNNN SNNNNNINNS
VNSVNSLMMN SDKNCNHQFP SDDVLISNEH DIIDLALNKE FLNQYRIQTN SIFINSQHVI
NQDVQSYQDY ESLLSSYNTI LNQLLLITSS NKNNNNNNNP NNNNNNVTSP NSLLSPPSTS
QLQQQQQQQQ QSPSPPLNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNY YLMSTSTMSS
TTTNLSTSTN SNINTNIPTN YNMSTSITAT TTTTTATNTI AIGTTTTAAI TTAATNSTNS
NNLNLSSTNL GLDNNFLYQR ILPEILSLLE KLTVFSRNND TEIFNRLGNN VKLIFDHLNL
IKNDILEANF SHLSGIFTIY NHTIISSDKT TKQFVFRFLH SLAPMTVPPA IVVTPELKKV
VQKIHFSKPT FTPLCIEWKE ICKYIDVLKS VSLFIAQVGQ YCFLNQSIII NDELLFIPSI
YVLFDNIQNL SMKEERFHIE YILHNLKFIV SKIPKQTREF TKTEIERLYS YIRGNPSYVK
EILPKLLKNI YIDPYFYHLW NNHCIHLLNN NINNNNNNNT NNSSNNSNNN NNTNINIGKD
IGNDLTLLLV SNKNSFLLSF DERHFSIFYS SISNSTTTTI TPTITSPNNN SNRINNNNNN
NNNNNNNNNN NNNINVNNNV NNNNVNNNNN NNNNNSNQYI VNYFDNTTSL LNFIESNQFK
YIILSNKEVE TIKFFKSVLP VTLPLPQPSS RQAILQQQQQ QNYKPRILCG PCLSRFKTIL
DEFDNEESIE NQIYSNYLNN INNNENIDPN LIDGNENKIN GNDNNNNHDD DEKTNNNNNN
NNNNNNNNNN NIYNNNNIED YNNNNDNNYN FENEINDKEN EFRELEKEIN EIEKEEQELI
KEYLELENRN QINLDIKQKL EDILKEIKLE EKTHYDLVNN YYQEYFDLKQ EDEIYTNQIN
AIRDQLERVS QIDINKITFK IELPNTGVNG GIVGYNESSN TTISSINGFR LGTLSNLKVD
WEEINSAWGE TSLLLYVLAS QLEFNFQNYK LIPMSSKSII QSKNDKMSYT LYGGDNIQFS
RSFLWFGASD QRFDSGMEAF LSCVNDIATF VQSKKPSINF NYRISKDKIG DSNRLLSIKI
AGNSELNWTM ALRFMLSNLK KILDNLDSIV ESKQKQQQLQ RQQSLNNL
