RXLRQ_PHYIT
ID RXLRQ_PHYIT Reviewed; 709 AA.
AC D0NRP3;
DT 08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2009, sequence version 1.
DT 03-AUG-2022, entry version 31.
DE RecName: Full=RxLR effector protein PITG_15110 {ECO:0000303|PubMed:30329083};
DE Flags: Precursor;
GN ORFNames=PITG_15110;
OS Phytophthora infestans (strain T30-4) (Potato late blight agent).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=403677;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND INDUCTION.
RC STRAIN=T30-4;
RX PubMed=19741609; DOI=10.1038/nature08358;
RG The Broad Institute Genome Sequencing Platform;
RA Haas B.J., Kamoun S., Zody M.C., Jiang R.H., Handsaker R.E., Cano L.M.,
RA Grabherr M., Kodira C.D., Raffaele S., Torto-Alalibo T., Bozkurt T.O.,
RA Ah-Fong A.M., Alvarado L., Anderson V.L., Armstrong M.R., Avrova A.,
RA Baxter L., Beynon J., Boevink P.C., Bollmann S.R., Bos J.I., Bulone V.,
RA Cai G., Cakir C., Carrington J.C., Chawner M., Conti L., Costanzo S.,
RA Ewan R., Fahlgren N., Fischbach M.A., Fugelstad J., Gilroy E.M., Gnerre S.,
RA Green P.J., Grenville-Briggs L.J., Griffith J., Grunwald N.J., Horn K.,
RA Horner N.R., Hu C.H., Huitema E., Jeong D.H., Jones A.M., Jones J.D.,
RA Jones R.W., Karlsson E.K., Kunjeti S.G., Lamour K., Liu Z., Ma L.,
RA Maclean D., Chibucos M.C., McDonald H., McWalters J., Meijer H.J.,
RA Morgan W., Morris P.F., Munro C.A., O'Neill K., Ospina-Giraldo M.,
RA Pinzon A., Pritchard L., Ramsahoye B., Ren Q., Restrepo S., Roy S.,
RA Sadanandom A., Savidor A., Schornack S., Schwartz D.C., Schumann U.D.,
RA Schwessinger B., Seyer L., Sharpe T., Silvar C., Song J., Studholme D.J.,
RA Sykes S., Thines M., van de Vondervoort P.J., Phuntumart V., Wawra S.,
RA Weide R., Win J., Young C., Zhou S., Fry W., Meyers B.C., van West P.,
RA Ristaino J., Govers F., Birch P.R., Whisson S.C., Judelson H.S.,
RA Nusbaum C.;
RT "Genome sequence and analysis of the Irish potato famine pathogen
RT Phytophthora infestans.";
RL Nature 461:393-398(2009).
RN [2]
RP INDUCTION.
RX PubMed=28228125; DOI=10.1186/s12864-017-3585-x;
RA Ah-Fong A.M., Kim K.S., Judelson H.S.;
RT "RNA-seq of life stages of the oomycete Phytophthora infestans reveals
RT dynamic changes in metabolic, signal transduction, and pathogenesis genes
RT and a major role for calcium signaling in development.";
RL BMC Genomics 18:198-198(2017).
RN [3]
RP INDUCTION, AND DOMAIN.
RX PubMed=29312401; DOI=10.3389/fpls.2017.02155;
RA Yin J., Gu B., Huang G., Tian Y., Quan J., Lindqvist-Kreuze H., Shan W.;
RT "Conserved RXLR effector genes of Phytophthora infestans expressed at the
RT early stage of potato infection are suppressive to host defense.";
RL Front. Plant Sci. 8:2155-2155(2017).
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=30329083; DOI=10.1093/jxb/ery360;
RA Wang S., McLellan H., Bukharova T., He Q., Murphy F., Shi J., Sun S.,
RA van Weymers P., Ren Y., Thilliez G., Wang H., Chen X., Engelhardt S.,
RA Vleeshouwers V., Gilroy E.M., Whisson S.C., Hein I., Wang X., Tian Z.,
RA Birch P.R.J., Boevink P.C.;
RT "Phytophthora infestans RXLR effectors act in concert at diverse
RT subcellular locations to enhance host colonization.";
RL J. Exp. Bot. 70:343-356(2019).
CC -!- FUNCTION: Effector that enhances P.infestans colonization of Nicotiana
CC benthamiana leaves. {ECO:0000269|PubMed:30329083}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:30329083}. Host
CC cytoplasm, host cytoskeleton. Note=Associates with host microtubules.
CC {ECO:0000269|PubMed:30329083}.
CC -!- INDUCTION: Expression is induced during host plant infection.
CC {ECO:0000269|PubMed:19741609, ECO:0000269|PubMed:28228125,
CC ECO:0000269|PubMed:29312401}.
CC -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC phosphate. {ECO:0000305|PubMed:29312401}.
CC -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR EMBL; DS028155; EEY63393.1; -; Genomic_DNA.
DR RefSeq; XP_002898278.1; XM_002898232.1.
DR AlphaFoldDB; D0NRP3; -.
DR SMR; D0NRP3; -.
DR EnsemblProtists; PITG_15110T0; PITG_15110T0; PITG_15110.
DR GeneID; 9475997; -.
DR KEGG; pif:PITG_15110; -.
DR VEuPathDB; FungiDB:PITG_15110; -.
DR eggNOG; ENOG502SRJH; Eukaryota.
DR HOGENOM; CLU_021192_3_0_1; -.
DR InParanoid; D0NRP3; -.
DR OMA; NTRRMAN; -.
DR Proteomes; UP000006643; Partially assembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0044163; C:host cytoskeleton; IEA:UniProtKB-SubCell.
PE 2: Evidence at transcript level;
KW Host cytoplasm; Host cytoskeleton; Reference proteome; Secreted; Signal;
KW Virulence.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..709
FT /note="RxLR effector protein PITG_15110"
FT /id="PRO_5003012319"
FT MOTIF 51..66
FT /note="RxLR-dEER"
FT /evidence="ECO:0000305|PubMed:29312401"
SQ SEQUENCE 709 AA; 79039 MW; A9DFA34C7BF474B5 CRC64;
MHAYSAAVLM GLLMVAEGAY VSTTACAHSA PNPTIQRYEP ESIPTRLAPN RLLREPETTE
ASNEDRVVSI HAGIEKLSDL IKTGVSKVHG YLNLGPSATR DQPADEILRI YKLDDGIEKA
LVSPNLKAME SHVKELSTKN RKSEASVIGI LTSHYGDDAV AKALVTAQKT VQSDDDVKTI
WRLRNAQLSS WFSSDKSVDD VFTLLKLRHD DYLALASPKM EVLDDYMKLI NRVTSGQETL
LNVLTKGFGG EQTMAKLLLR GKEEPQTREL ATALQNALLN KWVTDKFQPE SVLKKLKLDR
DLMNALSDPT RHTLTSYIAV FNTRNPGKKA SFIGTLSAHY GDEMVANVLI AASRNGNTRR
MANQLRTDQL SDWLNNQKSA DEVFSLLKLR ADLPNIDGAL ASGKLKLLED YIKLFNREKA
GDETLLKTLT TGFDGESNLA KALLTAEINP HSNKMVVKLQ GELLNQWLLK GLKPESVLKN
LGLDRGMKEV LSDPNRHFLT KYIWEYNSRN RFDRTSLILT LSAHYGDDVV ARALAVAKGD
SGLARTAAIL QRQQLEGWLS SGKSADDVFT LLRIGADDFL PLNSQNLETL EDFVWLLNLK
NRRIQTNIFT VVENKFGGDV QLARAVVKAL NEADERGLRD SVGIASRYRS KLFGRWFDKS
IEPKDVYAMI LKVNGASADA LEKSIVSRYT AFYKKRLAKA FTFDSPRRL