ID   RXLRR_PHYIT             Reviewed;         516 AA.
AC   D0NRQ7;
DT   08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2009, sequence version 1.
DT   03-AUG-2022, entry version 33.
DE   RecName: Full=RxLR effector protein PITG_15127 {ECO:0000303|PubMed:30329083};
DE   Flags: Precursor;
GN   ORFNames=PITG_15127;
OS   Phytophthora infestans (strain T30-4) (Potato late blight agent).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Phytophthora.
OX   NCBI_TaxID=403677;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=T30-4;
RX   PubMed=19741609; DOI=10.1038/nature08358;
RG   The Broad Institute Genome Sequencing Platform;
RA   Haas B.J., Kamoun S., Zody M.C., Jiang R.H., Handsaker R.E., Cano L.M.,
RA   Grabherr M., Kodira C.D., Raffaele S., Torto-Alalibo T., Bozkurt T.O.,
RA   Ah-Fong A.M., Alvarado L., Anderson V.L., Armstrong M.R., Avrova A.,
RA   Baxter L., Beynon J., Boevink P.C., Bollmann S.R., Bos J.I., Bulone V.,
RA   Cai G., Cakir C., Carrington J.C., Chawner M., Conti L., Costanzo S.,
RA   Ewan R., Fahlgren N., Fischbach M.A., Fugelstad J., Gilroy E.M., Gnerre S.,
RA   Green P.J., Grenville-Briggs L.J., Griffith J., Grunwald N.J., Horn K.,
RA   Horner N.R., Hu C.H., Huitema E., Jeong D.H., Jones A.M., Jones J.D.,
RA   Jones R.W., Karlsson E.K., Kunjeti S.G., Lamour K., Liu Z., Ma L.,
RA   Maclean D., Chibucos M.C., McDonald H., McWalters J., Meijer H.J.,
RA   Morgan W., Morris P.F., Munro C.A., O'Neill K., Ospina-Giraldo M.,
RA   Pinzon A., Pritchard L., Ramsahoye B., Ren Q., Restrepo S., Roy S.,
RA   Sadanandom A., Savidor A., Schornack S., Schwartz D.C., Schumann U.D.,
RA   Schwessinger B., Seyer L., Sharpe T., Silvar C., Song J., Studholme D.J.,
RA   Sykes S., Thines M., van de Vondervoort P.J., Phuntumart V., Wawra S.,
RA   Weide R., Win J., Young C., Zhou S., Fry W., Meyers B.C., van West P.,
RA   Ristaino J., Govers F., Birch P.R., Whisson S.C., Judelson H.S.,
RA   Nusbaum C.;
RT   "Genome sequence and analysis of the Irish potato famine pathogen
RT   Phytophthora infestans.";
RL   Nature 461:393-398(2009).
RN   [2]
RP   INDUCTION, AND DOMAIN.
RX   PubMed=29312401; DOI=10.3389/fpls.2017.02155;
RA   Yin J., Gu B., Huang G., Tian Y., Quan J., Lindqvist-Kreuze H., Shan W.;
RT   "Conserved RXLR effector genes of Phytophthora infestans expressed at the
RT   early stage of potato infection are suppressive to host defense.";
RL   Front. Plant Sci. 8:2155-2155(2017).
RN   [3]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=30329083; DOI=10.1093/jxb/ery360;
RA   Wang S., McLellan H., Bukharova T., He Q., Murphy F., Shi J., Sun S.,
RA   van Weymers P., Ren Y., Thilliez G., Wang H., Chen X., Engelhardt S.,
RA   Vleeshouwers V., Gilroy E.M., Whisson S.C., Hein I., Wang X., Tian Z.,
RA   Birch P.R.J., Boevink P.C.;
RT   "Phytophthora infestans RXLR effectors act in concert at diverse
RT   subcellular locations to enhance host colonization.";
RL   J. Exp. Bot. 70:343-356(2019).
CC   -!- FUNCTION: Effector that enhances P.infestans colonization of Nicotiana
CC       benthamiana leaves. {ECO:0000269|PubMed:30329083}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:30329083}. Host cell
CC       membrane {ECO:0000269|PubMed:30329083}. Host nucleus
CC       {ECO:0000269|PubMed:30329083}. Host cytoplasm
CC       {ECO:0000269|PubMed:30329083}.
CC   -!- INDUCTION: Expression is induced during host plant infection.
CC       {ECO:0000269|PubMed:29312401}.
CC   -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC       cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC       phosphate. {ECO:0000305|PubMed:29312401}.
CC   -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR   EMBL; DS028155; EEY63407.1; -; Genomic_DNA.
DR   RefSeq; XP_002898292.1; XM_002898246.1.
DR   AlphaFoldDB; D0NRQ7; -.
DR   SMR; D0NRQ7; -.
DR   EnsemblProtists; PITG_15127T0; PITG_15127T0; PITG_15127.
DR   GeneID; 9476011; -.
DR   KEGG; pif:PITG_15127; -.
DR   VEuPathDB; FungiDB:PITG_15127; -.
DR   eggNOG; ENOG502RFKP; Eukaryota.
DR   HOGENOM; CLU_021192_3_1_1; -.
DR   InParanoid; D0NRQ7; -.
DR   Proteomes; UP000006643; Partially assembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Host cell membrane; Host cytoplasm; Host membrane; Host nucleus; Membrane;
KW   Reference proteome; Secreted; Signal; Virulence.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..516
FT                   /note="RxLR effector protein PITG_15127"
FT                   /id="PRO_5003012808"
FT   MOTIF           48..63
FT                   /note="RxLR-dEER"
FT                   /evidence="ECO:0000305|PubMed:29312401"
SQ   SEQUENCE   516 AA;  57584 MW;  FCB9B6BE87CF6A35 CRC64;
     MRLYSGAILC TIATLLISVS TASKTTGSHL STRLPLPIDK PENNSLPRFL RVSTQNTENG
     ENRVISAGLE KLTDLAKAGV SNINLGSWIS KDPPAEPILR RFKLTNGMDD ALASPNLKAL
     ENYVKELNTQ NNNNKASVIG MFMAHYGDDA VANALMTAQR AGGEMNTIQR LRNAQLLSWL
     QKRKSVDDVF TLLKLRSDGY LALASPKMEV LDDYIKLVIS TKSTKDSLLK TLTKGFGGKE
     QLATILARAR EDVKTRELAL ALQSALINKW VRVDLLRPKH IYKKLRLNRG LDALLDRNVH
     TFGAFISMYN AKNPNSKASL IDSFVAQYGN KAVATALTFA KPDDAATMNL VITLQRQQFL
     KWKEGGISAN SVFRLLDIHF NDLLSPTNPK FDTLSGYLAT LSNKNLLYNE GMLRAVSKGF
     GGEDELAIQT ARAREHLIRN GYADLDEISI ATEYQRMLFG RWFKRKTKPS DIYGTTFRSS
     ENTASNLERA TAARYKDYYK EHMAPPRIDT SINPRR