ID   RXLRS_PHYIT             Reviewed;         519 AA.
AC   D0NQB4;
DT   08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2009, sequence version 1.
DT   03-AUG-2022, entry version 34.
DE   RecName: Full=RxLR effector protein PITG_15278 {ECO:0000303|PubMed:30329083};
DE   Flags: Precursor;
GN   ORFNames=PITG_15278;
OS   Phytophthora infestans (strain T30-4) (Potato late blight agent).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Phytophthora.
OX   NCBI_TaxID=403677;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=T30-4;
RX   PubMed=19741609; DOI=10.1038/nature08358;
RG   The Broad Institute Genome Sequencing Platform;
RA   Haas B.J., Kamoun S., Zody M.C., Jiang R.H., Handsaker R.E., Cano L.M.,
RA   Grabherr M., Kodira C.D., Raffaele S., Torto-Alalibo T., Bozkurt T.O.,
RA   Ah-Fong A.M., Alvarado L., Anderson V.L., Armstrong M.R., Avrova A.,
RA   Baxter L., Beynon J., Boevink P.C., Bollmann S.R., Bos J.I., Bulone V.,
RA   Cai G., Cakir C., Carrington J.C., Chawner M., Conti L., Costanzo S.,
RA   Ewan R., Fahlgren N., Fischbach M.A., Fugelstad J., Gilroy E.M., Gnerre S.,
RA   Green P.J., Grenville-Briggs L.J., Griffith J., Grunwald N.J., Horn K.,
RA   Horner N.R., Hu C.H., Huitema E., Jeong D.H., Jones A.M., Jones J.D.,
RA   Jones R.W., Karlsson E.K., Kunjeti S.G., Lamour K., Liu Z., Ma L.,
RA   Maclean D., Chibucos M.C., McDonald H., McWalters J., Meijer H.J.,
RA   Morgan W., Morris P.F., Munro C.A., O'Neill K., Ospina-Giraldo M.,
RA   Pinzon A., Pritchard L., Ramsahoye B., Ren Q., Restrepo S., Roy S.,
RA   Sadanandom A., Savidor A., Schornack S., Schwartz D.C., Schumann U.D.,
RA   Schwessinger B., Seyer L., Sharpe T., Silvar C., Song J., Studholme D.J.,
RA   Sykes S., Thines M., van de Vondervoort P.J., Phuntumart V., Wawra S.,
RA   Weide R., Win J., Young C., Zhou S., Fry W., Meyers B.C., van West P.,
RA   Ristaino J., Govers F., Birch P.R., Whisson S.C., Judelson H.S.,
RA   Nusbaum C.;
RT   "Genome sequence and analysis of the Irish potato famine pathogen
RT   Phytophthora infestans.";
RL   Nature 461:393-398(2009).
RN   [2]
RP   INDUCTION.
RX   PubMed=28228125; DOI=10.1186/s12864-017-3585-x;
RA   Ah-Fong A.M., Kim K.S., Judelson H.S.;
RT   "RNA-seq of life stages of the oomycete Phytophthora infestans reveals
RT   dynamic changes in metabolic, signal transduction, and pathogenesis genes
RT   and a major role for calcium signaling in development.";
RL   BMC Genomics 18:198-198(2017).
RN   [3]
RP   INDUCTION, AND DOMAIN.
RX   PubMed=29312401; DOI=10.3389/fpls.2017.02155;
RA   Yin J., Gu B., Huang G., Tian Y., Quan J., Lindqvist-Kreuze H., Shan W.;
RT   "Conserved RXLR effector genes of Phytophthora infestans expressed at the
RT   early stage of potato infection are suppressive to host defense.";
RL   Front. Plant Sci. 8:2155-2155(2017).
RN   [4]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=30329083; DOI=10.1093/jxb/ery360;
RA   Wang S., McLellan H., Bukharova T., He Q., Murphy F., Shi J., Sun S.,
RA   van Weymers P., Ren Y., Thilliez G., Wang H., Chen X., Engelhardt S.,
RA   Vleeshouwers V., Gilroy E.M., Whisson S.C., Hein I., Wang X., Tian Z.,
RA   Birch P.R.J., Boevink P.C.;
RT   "Phytophthora infestans RXLR effectors act in concert at diverse
RT   subcellular locations to enhance host colonization.";
RL   J. Exp. Bot. 70:343-356(2019).
CC   -!- FUNCTION: Effector that enhances P.infestans colonization of Nicotiana
CC       benthamiana leaves. {ECO:0000269|PubMed:30329083}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:30329083}. Host
CC       cytoplasm {ECO:0000269|PubMed:30329083}.
CC   -!- INDUCTION: Expression is induced during host plant infection.
CC       {ECO:0000269|PubMed:28228125, ECO:0000269|PubMed:29312401}.
CC   -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC       cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC       phosphate. {ECO:0000305|PubMed:29312401}.
CC   -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR   EMBL; DS028152; EEY62846.1; -; Genomic_DNA.
DR   RefSeq; XP_002898721.1; XM_002898675.1.
DR   AlphaFoldDB; D0NQB4; -.
DR   SMR; D0NQB4; -.
DR   EnsemblProtists; PITG_15278T0; PITG_15278T0; PITG_15278.
DR   GeneID; 9479248; -.
DR   KEGG; pif:PITG_15278; -.
DR   VEuPathDB; FungiDB:PITG_15278; -.
DR   eggNOG; ENOG502RG96; Eukaryota.
DR   HOGENOM; CLU_021192_3_2_1; -.
DR   InParanoid; D0NQB4; -.
DR   OMA; RYYQDHG; -.
DR   OrthoDB; 1679788at2759; -.
DR   Proteomes; UP000006643; Partially assembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
PE   2: Evidence at transcript level;
KW   Host cytoplasm; Reference proteome; Secreted; Signal; Virulence.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..519
FT                   /note="RxLR effector protein PITG_15278"
FT                   /id="PRO_5003013639"
FT   MOTIF           49..59
FT                   /note="RxLR-dEER"
FT                   /evidence="ECO:0000305|PubMed:29312401"
SQ   SEQUENCE   519 AA;  59726 MW;  30BBDFD7B088BD43 CRC64;
     MHFIYRVVLV LAAFALFKVD SISAGVTFNE EHLTIPRFTR PVEENLIKRQ LRVMDDNERG
     FPSGPSLEKI EAMFQSLTNK ITTKSKPSQR DVPDEKLSQL LGAAKPVNKA VEAAGRLKTL
     QTQKWLDEGK STQEVFQLLE LNKFMVPNFK KIEGTVFGLP DFNTWVNYVD DFNAKNPTKK
     ESMIPTLRTI YSDEGLTRAL ELAKTYSTTN ALATKLRKEQ IQRWLDDAQT PKYVFEMFMI
     DDKVDGLLTN PRFIAWTKYV DDFNLQYPTS RASMEPPIAA HYGDDAVFAM LEAAKKVQLT
     ENVASRLQAE QIKRLLNSNR SPEYVFEAFN LHETGDNLLS TPMFKTWFNY LESFNKKNTD
     KETLLAPIHR YYHDHGVAKI VAEGMKNPST VELSKQLQIQ RYKRWLHAER PPRDAFNTFI
     LEKPGADVII RYNERLDGKL YQVRLNKVSD GLLSSPEFQL WSKYLDDWNT KYPDQKQSMA
     KIFQALFTED ALAKMIMAAR NNPSTQKIAS LLENAFAKV