ID   RXLRV_PHYIT             Reviewed;         509 AA.
AC   D0NTC5;
DT   08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2009, sequence version 1.
DT   03-AUG-2022, entry version 33.
DE   RecName: Full=RxLR effector protein PITG_16195 {ECO:0000303|PubMed:30329083};
DE   Flags: Precursor;
GN   ORFNames=PITG_16195;
OS   Phytophthora infestans (strain T30-4) (Potato late blight agent).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Phytophthora.
OX   NCBI_TaxID=403677;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND INDUCTION.
RC   STRAIN=T30-4;
RX   PubMed=19741609; DOI=10.1038/nature08358;
RG   The Broad Institute Genome Sequencing Platform;
RA   Haas B.J., Kamoun S., Zody M.C., Jiang R.H., Handsaker R.E., Cano L.M.,
RA   Grabherr M., Kodira C.D., Raffaele S., Torto-Alalibo T., Bozkurt T.O.,
RA   Ah-Fong A.M., Alvarado L., Anderson V.L., Armstrong M.R., Avrova A.,
RA   Baxter L., Beynon J., Boevink P.C., Bollmann S.R., Bos J.I., Bulone V.,
RA   Cai G., Cakir C., Carrington J.C., Chawner M., Conti L., Costanzo S.,
RA   Ewan R., Fahlgren N., Fischbach M.A., Fugelstad J., Gilroy E.M., Gnerre S.,
RA   Green P.J., Grenville-Briggs L.J., Griffith J., Grunwald N.J., Horn K.,
RA   Horner N.R., Hu C.H., Huitema E., Jeong D.H., Jones A.M., Jones J.D.,
RA   Jones R.W., Karlsson E.K., Kunjeti S.G., Lamour K., Liu Z., Ma L.,
RA   Maclean D., Chibucos M.C., McDonald H., McWalters J., Meijer H.J.,
RA   Morgan W., Morris P.F., Munro C.A., O'Neill K., Ospina-Giraldo M.,
RA   Pinzon A., Pritchard L., Ramsahoye B., Ren Q., Restrepo S., Roy S.,
RA   Sadanandom A., Savidor A., Schornack S., Schwartz D.C., Schumann U.D.,
RA   Schwessinger B., Seyer L., Sharpe T., Silvar C., Song J., Studholme D.J.,
RA   Sykes S., Thines M., van de Vondervoort P.J., Phuntumart V., Wawra S.,
RA   Weide R., Win J., Young C., Zhou S., Fry W., Meyers B.C., van West P.,
RA   Ristaino J., Govers F., Birch P.R., Whisson S.C., Judelson H.S.,
RA   Nusbaum C.;
RT   "Genome sequence and analysis of the Irish potato famine pathogen
RT   Phytophthora infestans.";
RL   Nature 461:393-398(2009).
RN   [2]
RP   INDUCTION.
RX   PubMed=28228125; DOI=10.1186/s12864-017-3585-x;
RA   Ah-Fong A.M., Kim K.S., Judelson H.S.;
RT   "RNA-seq of life stages of the oomycete Phytophthora infestans reveals
RT   dynamic changes in metabolic, signal transduction, and pathogenesis genes
RT   and a major role for calcium signaling in development.";
RL   BMC Genomics 18:198-198(2017).
RN   [3]
RP   INDUCTION, AND DOMAIN.
RX   PubMed=29312401; DOI=10.3389/fpls.2017.02155;
RA   Yin J., Gu B., Huang G., Tian Y., Quan J., Lindqvist-Kreuze H., Shan W.;
RT   "Conserved RXLR effector genes of Phytophthora infestans expressed at the
RT   early stage of potato infection are suppressive to host defense.";
RL   Front. Plant Sci. 8:2155-2155(2017).
RN   [4]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=30329083; DOI=10.1093/jxb/ery360;
RA   Wang S., McLellan H., Bukharova T., He Q., Murphy F., Shi J., Sun S.,
RA   van Weymers P., Ren Y., Thilliez G., Wang H., Chen X., Engelhardt S.,
RA   Vleeshouwers V., Gilroy E.M., Whisson S.C., Hein I., Wang X., Tian Z.,
RA   Birch P.R.J., Boevink P.C.;
RT   "Phytophthora infestans RXLR effectors act in concert at diverse
RT   subcellular locations to enhance host colonization.";
RL   J. Exp. Bot. 70:343-356(2019).
CC   -!- FUNCTION: Effector that enhances P.infestans colonization of Nicotiana
CC       benthamiana leaves. {ECO:0000269|PubMed:30329083}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:30329083}. Host cell
CC       membrane {ECO:0000269|PubMed:30329083}.
CC   -!- INDUCTION: Expression is induced during host plant infection.
CC       {ECO:0000269|PubMed:19741609, ECO:0000269|PubMed:28228125,
CC       ECO:0000269|PubMed:29312401}.
CC   -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC       cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC       phosphate. {ECO:0000305|PubMed:29312401}.
CC   -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR   EMBL; DS028160; EEY64876.1; -; Genomic_DNA.
DR   RefSeq; XP_002897606.1; XM_002897560.1.
DR   AlphaFoldDB; D0NTC5; -.
DR   SMR; D0NTC5; -.
DR   EnsemblProtists; PITG_16195T0; PITG_16195T0; PITG_16195.
DR   GeneID; 9465370; -.
DR   KEGG; pif:PITG_16195; -.
DR   VEuPathDB; FungiDB:PITG_16195; -.
DR   eggNOG; ENOG502SZKM; Eukaryota.
DR   HOGENOM; CLU_021192_3_0_1; -.
DR   InParanoid; D0NTC5; -.
DR   OMA; AYHYSDE; -.
DR   OrthoDB; 1190068at2759; -.
DR   Proteomes; UP000006643; Partially assembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Host cell membrane; Host membrane; Membrane; Reference proteome; Secreted;
KW   Signal; Virulence.
FT   SIGNAL          1..28
FT                   /evidence="ECO:0000255"
FT   CHAIN           29..509
FT                   /note="RxLR effector protein PITG_16195"
FT                   /id="PRO_0000446909"
FT   MOTIF           53..63
FT                   /note="RxLR-dEER"
FT                   /evidence="ECO:0000305|PubMed:29312401"
SQ   SEQUENCE   509 AA;  56819 MW;  C6BF324304F5A16C CRC64;
     MSTARTQPSV TKILATVIIL ACINVVSTAS TSKLVRIGSF AANSDLTEVS HRRFLRTSVA
     EERKLALSFP WLGQAVSGTQ SWAATLLQTL QQKWSQMRMK SPNDMFKKLK LDNTGDQLFS
     SPRFSKWLSY VRTNSKTNPD MAIFSTLAYH YSDEALVKLL DAAKKVDSTK VLATKLEGLQ
     FTNWVHARES PEYVFKVLAL DRMGSNTFTS PQFSKWLSYM NKAETSDPEM AIYRVLGTYH
     SDDVLVKMFA AAKQAESTRA LASSLEKVQF ENWARGGESP SHVFKALALD QMDTQIFASP
     QFSKWTSFVS KANTKNPDVA MYTTLGTFYS DDILAKMFAA GKQVDSTKGL ATRLEGIQLA
     NWENAGKSAE SAFKTLKLDT LPGSQLFESQ FINTWASFVT RTHKDPDAIM VALLKDRYGD
     ETLAKMIAAA TKTERTEKLA VDLRSAQFKT WFSQGKTPEN VNTLFKVAAN SDDLTKKISR
     EYDIFFSKSK VAFNRPANRP ARNGIYIAG