ID   RXLRW_PHYIT             Reviewed;         678 AA.
AC   D0NVF3;
DT   08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2009, sequence version 1.
DT   03-AUG-2022, entry version 36.
DE   RecName: Full=RxLR effector protein PITG_16705 {ECO:0000303|PubMed:30329083};
DE   Flags: Precursor;
GN   ORFNames=PITG_16705;
OS   Phytophthora infestans (strain T30-4) (Potato late blight agent).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Phytophthora.
OX   NCBI_TaxID=403677;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND INDUCTION.
RC   STRAIN=T30-4;
RX   PubMed=19741609; DOI=10.1038/nature08358;
RG   The Broad Institute Genome Sequencing Platform;
RA   Haas B.J., Kamoun S., Zody M.C., Jiang R.H., Handsaker R.E., Cano L.M.,
RA   Grabherr M., Kodira C.D., Raffaele S., Torto-Alalibo T., Bozkurt T.O.,
RA   Ah-Fong A.M., Alvarado L., Anderson V.L., Armstrong M.R., Avrova A.,
RA   Baxter L., Beynon J., Boevink P.C., Bollmann S.R., Bos J.I., Bulone V.,
RA   Cai G., Cakir C., Carrington J.C., Chawner M., Conti L., Costanzo S.,
RA   Ewan R., Fahlgren N., Fischbach M.A., Fugelstad J., Gilroy E.M., Gnerre S.,
RA   Green P.J., Grenville-Briggs L.J., Griffith J., Grunwald N.J., Horn K.,
RA   Horner N.R., Hu C.H., Huitema E., Jeong D.H., Jones A.M., Jones J.D.,
RA   Jones R.W., Karlsson E.K., Kunjeti S.G., Lamour K., Liu Z., Ma L.,
RA   Maclean D., Chibucos M.C., McDonald H., McWalters J., Meijer H.J.,
RA   Morgan W., Morris P.F., Munro C.A., O'Neill K., Ospina-Giraldo M.,
RA   Pinzon A., Pritchard L., Ramsahoye B., Ren Q., Restrepo S., Roy S.,
RA   Sadanandom A., Savidor A., Schornack S., Schwartz D.C., Schumann U.D.,
RA   Schwessinger B., Seyer L., Sharpe T., Silvar C., Song J., Studholme D.J.,
RA   Sykes S., Thines M., van de Vondervoort P.J., Phuntumart V., Wawra S.,
RA   Weide R., Win J., Young C., Zhou S., Fry W., Meyers B.C., van West P.,
RA   Ristaino J., Govers F., Birch P.R., Whisson S.C., Judelson H.S.,
RA   Nusbaum C.;
RT   "Genome sequence and analysis of the Irish potato famine pathogen
RT   Phytophthora infestans.";
RL   Nature 461:393-398(2009).
RN   [2]
RP   INDUCTION.
RX   PubMed=28228125; DOI=10.1186/s12864-017-3585-x;
RA   Ah-Fong A.M., Kim K.S., Judelson H.S.;
RT   "RNA-seq of life stages of the oomycete Phytophthora infestans reveals
RT   dynamic changes in metabolic, signal transduction, and pathogenesis genes
RT   and a major role for calcium signaling in development.";
RL   BMC Genomics 18:198-198(2017).
RN   [3]
RP   INDUCTION, AND DOMAIN.
RX   PubMed=29312401; DOI=10.3389/fpls.2017.02155;
RA   Yin J., Gu B., Huang G., Tian Y., Quan J., Lindqvist-Kreuze H., Shan W.;
RT   "Conserved RXLR effector genes of Phytophthora infestans expressed at the
RT   early stage of potato infection are suppressive to host defense.";
RL   Front. Plant Sci. 8:2155-2155(2017).
RN   [4]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=30329083; DOI=10.1093/jxb/ery360;
RA   Wang S., McLellan H., Bukharova T., He Q., Murphy F., Shi J., Sun S.,
RA   van Weymers P., Ren Y., Thilliez G., Wang H., Chen X., Engelhardt S.,
RA   Vleeshouwers V., Gilroy E.M., Whisson S.C., Hein I., Wang X., Tian Z.,
RA   Birch P.R.J., Boevink P.C.;
RT   "Phytophthora infestans RXLR effectors act in concert at diverse
RT   subcellular locations to enhance host colonization.";
RL   J. Exp. Bot. 70:343-356(2019).
CC   -!- FUNCTION: Effector that enhances P.infestans colonization of Nicotiana
CC       benthamiana leaves. {ECO:0000269|PubMed:30329083}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:30329083}. Host
CC       cytoplasm {ECO:0000269|PubMed:30329083}.
CC   -!- INDUCTION: Expression is induced during host plant infection.
CC       {ECO:0000269|PubMed:19741609, ECO:0000269|PubMed:28228125,
CC       ECO:0000269|PubMed:29312401}.
CC   -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC       cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC       phosphate. {ECO:0000305|PubMed:29312401}.
CC   -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR   EMBL; DS028168; EEY66630.1; -; Genomic_DNA.
DR   RefSeq; XP_002896931.1; XM_002896885.1.
DR   AlphaFoldDB; D0NVF3; -.
DR   SMR; D0NVF3; -.
DR   EnsemblProtists; PITG_16705T0; PITG_16705T0; PITG_16705.
DR   GeneID; 9465494; -.
DR   KEGG; pif:PITG_16705; -.
DR   VEuPathDB; FungiDB:PITG_16705; -.
DR   eggNOG; ENOG502RANW; Eukaryota.
DR   HOGENOM; CLU_021192_3_0_1; -.
DR   InParanoid; D0NVF3; -.
DR   OMA; SAYNARY; -.
DR   OrthoDB; 866049at2759; -.
DR   Proteomes; UP000006643; Partially assembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
PE   2: Evidence at transcript level;
KW   Host cytoplasm; Reference proteome; Secreted; Signal; Virulence.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..678
FT                   /note="RxLR effector protein PITG_16705"
FT                   /id="PRO_5003013103"
FT   MOTIF           46..61
FT                   /note="RxLR-dEER"
FT                   /evidence="ECO:0000305|PubMed:29312401"
SQ   SEQUENCE   678 AA;  76870 MW;  538CC307FB0F8FCC CRC64;
     MHLFFLTAVA FVITSVSVDA SVAKDPRGHA PNRTEVDTVN ASSSTRLLRK NSTVDLVGEE
     RAPSIVENIK ALVKSSAVTP AKLQQWLDER LPAGLVFKNM NLDEPNIFSL LHEPNFAKWV
     QYADDLSAKS SHKESSVIST LTSLHGDKVV YDTIQAAKLY PQLSELALKL EKDQIRFWIA
     TRKDPSVVFE ALNLNWAGIS IFPKPEFSAW LKYVDDVNAR HPKEAPLSII PTLKQRFSRG
     DEAGTDVLLK LIANGKATTE AKTVANKVES ALFDFWLNSR ETPDKVMDAF KYGTTTQAFL
     GSPRWKEWER YLSAYNARYP EKKATAIETL TRKYGDAQLL DTLIGASSKG ETKTLAAKLQ
     AQQFDRWMNL KESPLDVYNR LRSSYGDTAF FNEPQLNVWV SYMNVFVDKN PSKVDKMFLE
     LGDTFGDMRL FRVLGEAKKF PNLESTATKL QMEKASTLFA SGKSPEGIFK VLALDNVGDD
     ILSNTLFHKW LAYLQKFNKE HPNNQESWFD MLRISYQPFG VERIIETGRK NPLTRLMAEK
     VENAYHNYWL DIKMEPKTAF RSLHLDESGE KLLADPKFNT WVQYLKTFND RYPNEKTTVI
     DGLRDNSHDI ALLRMFSAAK NDPSTEKLAT DLQSALILKW QDAKKTPEEL KRVFVGVPAA
     DEMLDRYIKL LAVASSTP